Rhizobium Scot1305 and application of rhizobium Scot1305 in promoting phytoremediation of heavy metal pollution of soil

A phytoremediation and rhizobia technology, applied in the field of microorganisms, can solve the problems of serious heavy metal poisoning and low restoration efficiency of plants, achieve good soil restoration effect, enhance achievability, and improve soil restoration effect.

Active Publication Date: 2016-11-30
SICHUAN INST OF ATOMIC ENERGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem of severe heavy metal poisoning and low repair efficiency in phytoremediation in the prior art, and provides a rhizobia Scot1305 for the current situation of soil pollution by heavy metal Co in China.

Method used

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  • Rhizobium Scot1305 and application of rhizobium Scot1305 in promoting phytoremediation of heavy metal pollution of soil
  • Rhizobium Scot1305 and application of rhizobium Scot1305 in promoting phytoremediation of heavy metal pollution of soil
  • Rhizobium Scot1305 and application of rhizobium Scot1305 in promoting phytoremediation of heavy metal pollution of soil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation, culture, physical and chemical characteristics of rhizobia Scot1305 and its protective effect on key enzymes of plant tocopherol synthesis.

[0026] The rhizobia Scot1305 of the present invention is screened from leguminous plant leech samples in the Lala copper mine area of ​​Panzhihua, Sichuan.

[0027] 1. Morphological characteristics of Rhizobium Scot1305:

[0028] A strain of rhizobium was screened from the root nodules of leeches by separation and purification, and its strain number was Scot1305. Scot1305 in YEM medium (proportion by mass: mannitol 10.0g, yeast powder 1g, K 2 HPO 4 0.5g, MgSO 4 0.2g, NaCl0.1g, CaCl 2 Solution (0.325g / L) 2mL, FeCl 3 .6H 2 O solution (0.01g / mL) 1mL, trace element solution 1mL, add ultrapure water to 1L, pH7.0~7.2. Trace element solution (g / L): H 3 BO 3 2.86g, MnSO 4 1.81g, ZnSO 4 0.22g, CuSO 4 0.80g, H 2 MoO 2 0.02g) after being cultured on a plate for 36 hours, colonies with a diameter of 0.8-1.5 mm can be ...

Embodiment 2

[0036] Example 2: Using Rhizobium Scot1305 to simulate remediation of heavy metal Co pollution in soil

[0037] (1) Seedling preparation: the test plant is a leguminous plant - alfalfa Medicago sativa , divided into inoculation group and control group, the aseptic treatment method of seeds is: 70% ethanol disinfection for 1 min, soaking in 0.1% mercuric chloride for 5-10 min, and rinsing with sterile water for 3-5 times. Inoculation group seeds use OD 600 =1.0 Rhizobia Scot1305 bacterial solution soaked in 50mL for 30-60min; the seeds of the control group were soaked in 50mL sterile water for the same time, and transferred to the culture medium.

[0038] (2) Cultivation medium: The culture medium is 50% coconut soil + 40% nutrient soil + 10% perlite, sterilized by high-pressure steam at 121°C for 2 hours, after sterilization, the matrix and 1 / 2 MS liquid medium are mixed according to the ratio of 1:3 (w / v) mixing, for the cultivation of plants.

[0039] (3) Growth managem...

Embodiment 3

[0045] Embodiment 3: Use Rhizobium Scot1305 to remediate heavy metal Co pollution in soil

[0046] (1) Seedling preparation: the test plant is alfalfa Medicago sativa , divided into three groups: control group, contamination group and inoculation group. The seed sterilization treatment method is: 70% ethanol disinfection for 1 minute, soaking in 0.1% mercury liter for 5-10 minutes, and rinsing with sterile water for 3-5 times. Sow in the test fields of greenhouses, cut off the aboveground part after growing for 8 months, dig out the roots, select plants of uniform size, clean them and set them aside.

[0047] (2) Test soil: the pollution group and the inoculation group used the river bank soil of the Luxi River Basin in Chengdu, and cobalt chloride (CoCl 2 ) mixed evenly, and the soil was naturally placed for 18 months to fully mature. The average content of Co in the polluted soil was 231.1 mg / kg, and the soil in the control group was the same batch of sampled soil withou...

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Abstract

The invention provides rhizobium Scot1305 and a symbiotic system of rhizobium Scot1305 and plants is established. Due to nitrogen fixation function, siderophore secretion function and soluble phosphorus activity of the rhizobium strain and protection effect of the rhizobium strain on key enzyme of plant tocopherol synthesis, enhancement effect of the strain on phytoremediation of Co contaminated soil is realized. The strain can coexist with plants in Co contaminated soil, and can promote the extraction and enrichment of heavy metals Co in the aerial parts of plants, and finally achieve the purpose that the content of Co in soil meets the environmental safety standard.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a rhizobia ( Rhizobium sp. ) Scot1305 and its application in promoting phytoremediation of heavy metal pollution in soil. Background technique [0002] The heavy metal elements that naturally exist in nature are mostly lower than their pollution concentrations. However, due to human activities such as the mining, smelting, processing and commercial manufacturing of heavy metals, the excessive accumulation of various heavy metals such as lead, mercury, cadmium, cobalt (Co) and other metal elements in the soil has caused heavy metal pollution in the soil. pollute. In China, the background value of the average Co content in soil is 11.6 mg / kg, and the Co content in most soils is 5-40 mg / kg, while the Co content in polluted soil is more than 100 mg / kg. At present, there are few studies on the phytoremediation technology of heavy metal Co pollution. How to effectively reme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/41
CPCB09C1/10B09C2101/00C12N1/205C12R2001/41
Inventor 刘绵学伏毅黄敏陈浩
Owner SICHUAN INST OF ATOMIC ENERGY
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