Molecular improvement method for lowering rice grain seed holding

Abstract

The invention discloses a molecular genetic improvement method for lowering rice seed holding by targeted modification on rice seed holding gene qSH1 by using a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 system. The method comprises the following steps: 1) selecting an appropriate target; 2) establishing a vector containing the target spot sequence; 3) establishing a recombinant vector containing the target spot sequence by utilizing the vector; 4) introducing the recombinant vector into receptor rice to obtain a transgenic positive plant; 5) obtaining a targeted-mutation mutant plant by utilizing the transgenic positive plant; 6) carrying out additive-generation growth on the mutant plant to obtain a transgenic-component-free homozygous mutant plant; and 7) carrying out seed holding investigation by using the homozygous mutant plant to obtain the plant with obviously lower seed holding as the seed-holding-improved plant. The method has the advantages of high directionality, small genetic background changes and the like, can avoid the risks caused by genetic transformation, and can culture the new species and new combination of transgenic-component-free rice with obviously lower seed holding.

Description

technical field [0001] The invention belongs to the field of rice biotechnology breeding, and in particular relates to a molecular genetic improvement method for using a CRISPR / Cas9 system to target-modify qSH1 to reduce rice grain shattering. Background technique [0002] Rice (Oryza sativa L.) is an important food crop in the world, and more than half of the world's population uses rice as the main food. In my country, rice is also the most important food crop. As the largest ration crop, about 65% of my country's population takes rice as a staple food. However, with the increase of population and the decrease of arable land, the problem of imbalance between supply and demand of food has become increasingly serious. Therefore, rice production is closely related to economic development, social stability, and national security, and it plays a pivotal role in ensuring my country's food security. [0003] Rice grain shattering is one of the important agronomic traits closely...

AI Technical Summary

Problems solved by technology

However, for the common cultivated rice varieties (combinations) that are currently used in actual production by humans, the excessive grain shattering will cause a large loss of harvest yield due to a large number of grains falling in the field when harvesting, so it is not popular.

Although after artificial domestication and improvement, the grain shattering of most cultivated rice varieties is relatively moderate, there are still some varieties, especially some backbone parents of indica hybrid rice and their combinations (such as R1128, Guangliangyou 1128, etc.) Reduced harvest yield due to easy kernel drop

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