Application of tobacco NtNAC096 gene in control of tobacco aging

Abstract

The invention provides application of a tobacco NtNAC096 gene in control tobacco aging, belonging to the field of tobacco biological control. The tobacco NtNAC096 gene can be used for regulating and controlling the aging of tobaccos significantly. The full length of a DNA sequence of a genome using the tobacco NtNAC096 gene c is 1203bp, and the genome consists of 3 exons and 2 introns. The application specifically comprises the following steps: (1) cloning the tobacco NtNAC096 gene, and performing sequence analysis; (2) performing expression analysis on the NtNAC096 gene; (3) performing function analysis on the tobacco NtNAC096 gene by establishing an over-expression vector of the tobacco NtNAC096 gene; and (4) designing target sites of the tobacco NtNAC096 gene by using a CRISPR / Cas9 gene editing technology, establishing a NtNAC096 gene knockout vector, and performing genetic transformation on a tobacco variety, thereby obtaining a tobacco plant capable of delaying aging.

Description

technical field [0001] The invention relates to the field of tobacco aging control, in particular to an application of tobacco NtNAC096 gene to control tobacco aging. Background technique [0002] Leaf senescence is the last stage of leaf development. During leaf senescence, chlorophyll and biomacromolecular substances such as proteins, lipids, nucleic acids, etc. are degraded, the photosynthetic capacity of leaves is reduced, and nutrients in aging tissues are degraded and transformed and transported to young tissues and reproductive organs for further growth development or storage. Therefore, leaf senescence affects crop growth, nutrient accumulation and yield formation. Tobacco is a leaf economic crop, the leaf area size and senescence maturity directly affect the production yield and quality. [0003] At present, the control of plant senescence mainly focuses on the regulation of plant hormones. Auxin, cytokinin, etc. can inhibit leaf senescence, and ethylene and absc...

AI Technical Summary

Problems solved by technology

However, hormone regulation of exogenous effects often has defects in the effect

In view of this, the method of using genes to control senescence has begun to be studied. At present, only a few tobacco senescence genes have been identified, and there are still a large number of plant senescence-related genes to be studied and utilized.

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