In-vitro regeneration culture method for tea clones
A technology of in vitro regeneration and regeneration medium, which is applied in the field of establishment of tea tree tissue culture regeneration system, can solve the problems of difficulty in meeting the needs of genetic transformation and low regeneration rate of explants, and achieve high regeneration rate, high regeneration coefficient, and callus The effect of high regeneration coefficient
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[0042] (1) Selection of explants
[0043]The applicant collected the new shoots of the tea [Camellia sinensis (L.) O. Kuntze] variety 'Longjing 43' from the tea garden of the Agricultural Garden of Anhui Agricultural University from April to May in spring, cut off the leaves and left the petioles to protect the buds, and washed them with tap water; Transfer to tap water dripping with 2-3 drops of Liby detergent per liter and rinse for 15-20min, then rinse with tap water and use it as an explant regenerated in vitro for later use. The test results showed that the use of new shoots with appropriate maturity as explants could reduce browning and increase the survival rate.
[0044] (2) kill surface bacteria
[0045] Cut the cleaned new shoots into single-bud stem segments with buds, immerse them in 70% ethanol solution for 30s in the ultra-clean bench, shake while soaking; then place them in 0.1% mercury chloride (HgCl 2 ) (containing 0.1% Tween) solution soaked for 7-10min, an...
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