In-vitro regeneration culture method for tea clones

A technology of in vitro regeneration and regeneration medium, which is applied in the field of establishment of tea tree tissue culture regeneration system, can solve the problems of difficulty in meeting the needs of genetic transformation and low regeneration rate of explants, and achieve high regeneration rate, high regeneration coefficient, and callus The effect of high regeneration coefficient

Inactive Publication Date: 2012-04-25
ANHUI AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

In summary, the regeneration rate of explants in the regeneration system established on tea clone

Method used

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  • In-vitro regeneration culture method for tea clones
  • In-vitro regeneration culture method for tea clones
  • In-vitro regeneration culture method for tea clones

Examples

Experimental program
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Embodiment

[0042] (1) Selection of explants

[0043]The applicant collected the new shoots of the tea [Camellia sinensis (L.) O. Kuntze] variety 'Longjing 43' from the tea garden of the Agricultural Garden of Anhui Agricultural University from April to May in spring, cut off the leaves and left the petioles to protect the buds, and washed them with tap water; Transfer to tap water dripping with 2-3 drops of Liby detergent per liter and rinse for 15-20min, then rinse with tap water and use it as an explant regenerated in vitro for later use. The test results showed that the use of new shoots with appropriate maturity as explants could reduce browning and increase the survival rate.

[0044] (2) kill surface bacteria

[0045] Cut the cleaned new shoots into single-bud stem segments with buds, immerse them in 70% ethanol solution for 30s in the ultra-clean bench, shake while soaking; then place them in 0.1% mercury chloride (HgCl 2 ) (containing 0.1% Tween) solution soaked for 7-10min, an...

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Abstract

The invention discloses an in-vitro regeneration culture method for tea clones, comprising the steps of selecting an explant, killing surface bacteria, carrying out primary culture, carrying out secondary culture, extending shoots and carrying out rooting culture Tea[Camellia sinensis(L.)O.Kuntze] is a perennial woody crop which is rich in healthy secondary substances, the seed of tea is a sexual hybrid and has self-incompatibility. A traditional breeding method has the defects of long period and low efficiency, and can not realize the orderly rapid improvement of the species. The regeneration method of clones in the prior art has low regeneration rate, and can not satisfy the requirements of genetic transformation for a high frequency regeneration system. The method disclosed in the invention is simple and convenient for operating, has high regeneration efficiency and improved regeneration system, and provides important technical supports for breeding novel species of tea by using modern biological techniques.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for establishing a tea tree tissue culture regeneration system. Background technique [0002] Tea [Camellia sinensis (L.) O.Kuntze] is one of the three major beverages in the world. It has pharmacological and health functions because it is rich in theanine, catechin and caffeine. Tea tree production often faces severe challenges due to various adversities such as low temperature and pests, causing producers and enterprises to suffer serious losses. [0003] Tea is self-incompatible, the genetic background is highly complex, and because of the low seed setting rate, hybrid seeds are often not obtained, which brings great difficulties to breeding research. Therefore, tea tree breeding is still dominated by single plant selection, which makes tea The breeding work is not only long-term and difficult, but also blind. Clonal genetic transformation c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 孙俊张正竹宛晓春雷攀登汤志近石冠华朱善玉
Owner ANHUI AGRICULTURAL UNIVERSITY
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