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Method for establishing tissue culture regeneration system of camellia sinensis L.

A tissue culture and construction method technology, applied in the field of plant tissue culture, can solve the problems of browning of explants, difficulty in rooting, application limitations of tissue culture technology, etc., and achieve the effect of solving the shortage

Inactive Publication Date: 2015-06-10
刘木娇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some progress has been made in tea tree tissue culture research, but there are still some problems, such as serious browning of explants, low shoot rate, difficult rooting, low survival rate of transplanted field, etc. Applications in the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Seed disinfection: Select immature tea fruit at the end of August, soak in washing powder water for 10 minutes after removing leaves, rinse with tap water for 1 hour after brushing, take out immature milky white or slightly brown seeds, and put them in an ultra-clean workbench. Peel off the outer testa, first sterilize with 75% ethanol for 5 seconds, then wash 3 times with sterile water, then sterilize with 0.1% mercuric chloride solution for 10 minutes, rinse with sterile water 4 times and set aside, the statistical pollution rate can be as low as 3 %.

[0018] (2) Embryo callus induction: the immature embryos treated in step (1) were cut into 0.5cm×0.5cm×0.5cm and inoculated on the induction medium, and cultured at 28°C in total darkness for 30 days. Then put it under light for 10 hours every day, and the light intensity is 1500x, until embryogenic callus is induced, and the callus induction rate is 67%. The induction medium is ER+6-BA1.0mg / L+NAA0.5mg / L+GA 3 5 ...

Embodiment 2

[0023] (1) Seed disinfection: select immature tea fruit at the end of August, soak in washing powder water for 15 minutes after removing leaves, rinse with tap water for 2 hours after brushing, take out immature milky white or slightly brown seeds, and put them in an ultra-clean workbench. Peel off the outer testa, first sterilize with 75% ethanol for 8 seconds, then wash 3 times with sterile water, then sterilize with 0.1% mercuric chloride solution for 12 minutes, rinse with sterile water 4 times and then set aside. The statistical pollution rate can be as low as 5% after one week of inoculation. %.

[0024] (2) Embryo callus induction: the immature embryos treated in step (1) were cut into 0.5cm×0.5cm×0.5cm and inoculated on the induction medium, and cultured at 28°C in total darkness for 30 days. Then placed in light for 12 hours a day, the light intensity was 1500x, until embryogenic callus was induced, and the callus induction rate was 63%. The induction medium is ER+6-...

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Abstract

The invention discloses a method for establishing a tissue culture regeneration system of camellia sinensis L. and relates to a technical method for obtaining strain property stable camellia sinensis L. seedlings from the camellia sinensis L. by use of a vegetative rapid propagation technique. According to the method, the tissue culture regeneration system of camellia sinensis L. is established by taking the immaturate embryo of the camellia sinensis L. as the explant and by virtue of the steps of seed sterilization, embryo callus induction, differentiation culture, rooting culture, acclimatization and transplant and the like; as a result, the problem of shortage of good-quality seedlings of the camellia sinensis L. is solved, and a foundation also can be laid for developing the genetic transformation work of camellia sinensis L. in the future.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for constructing a tea tree tissue culture regeneration system. Background technique [0002] Tea( Camellia sinensis L.) is a perennial evergreen tree or shrub of the family Theaceae Camellia, which has a long history of cultivation and is one of the important economic crops in my country. Tea trees prefer light and fear sun, and love temperature and fear cold, so they have certain requirements for soil moisture content. my country's camellia plant is rich in resources, and it is an economic crop for greening barren hills and maintaining water and soil in South China. [0003] Conventional tea tree breeding technology has limitations, such as the difficulty of distant hybridization, low frequency of beneficial mutations, long time-consuming breeding of fine varieties, and rare flowering period, etc., which restricts the utilization ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘木娇
Owner 刘木娇
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