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A method for rapid propagation of trigeminus, a homologous Lingnan medicinal and edible plant

A technology of medicinal and edible homologous plants, trigeminin, which is applied in the field of plant tissue culture, can solve the problems that there is no patent application for tissue culture and rapid propagation of trigeminus, there is no tissue culture technology of trigeminin, and the reproduction coefficient is low, so as to solve the problem of plant tissue culture The effect of lack of seedlings, easy handling, and high reproduction coefficient

Inactive Publication Date: 2019-04-05
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Trigemina is mainly propagated by seeds and cuttings. However, due to the hard seeds and low germination rate of Trichosanthes, the cutting propagation has the disadvantages of long cycle and low reproduction coefficient. The establishment of tissue culture and rapid propagation technology of Trichasin has become the first priority for large-scale planting. Solved technology
[0004] At present, there is no report on the tissue culture technology of trigeminus at home and abroad, and there is no application for the patent of tissue culture and rapid propagation of trigeminus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Preparation of explants: In the autumn of 2014, a vigorously growing Tridentia chinensis plant was selected in the wild, and the root was circumcised at a position about 5cm above the ground. In the spring of 2015, 6 to 9 new vegetative buds were formed at the circumcision. .

[0022] (2) Collection of explants: In spring, when the vegetative buds at the circumcision of the trigeminus plant processed in step (1) are generated, the vegetative buds are cut from the base with a sterile scalpel and taken back to the laboratory in time after simple moisturizing and water-retaining treatment .

[0023] (3) Adventitious bud induction: the explants taken back to the laboratory were first rinsed with tap water overnight, and then sterilized in 75% ethanol solution in an ultra-clean workbench for 15 seconds, rinsed twice with sterile water, and sucked with sterile filter paper. After drying the water, put it in 0.1% mercuric chloride solution for disinfection for 10 minutes,...

Embodiment 2

[0029] (1) Preparation of explants: In the autumn of 2015, a vigorously growing Tridentia chinensis plant was selected in the wild, and the root was circumcised at a place about 8 cm above the ground. In the spring of 2016, 6 to 7 newborn plants were formed at the circumcision site. Nutritious buds.

[0030] (2) Collection of explants: In the spring of 2016, when the vegetative buds at the circumcision of the trigeminal bitter plants treated in step (1) were formed, the vegetative buds were cut from the base with a sterile scalpel and brought back in time after simple moisturizing and water-retaining treatment laboratory.

[0031] (3) Adventitious bud induction: the explants taken back to the laboratory were first rinsed with tap water overnight, and then sterilized in 75% ethanol solution in an ultra-clean workbench for 23 seconds, rinsed with sterile water for 3 times, and sucked with sterile filter paper. After drying the water, put it in 0.1% mercuric chloride solution fo...

Embodiment 3

[0037] (1) Preparation of explants: In the autumn of 2015, a vigorously growing Tridentia chinensis plant was selected in the wild, and the root was circumcised at a place about 10 cm above the ground. In the spring of 2016, 5 to 6 new vegetative plants were formed at the circumcision treatment. bud.

[0038] (2) Collection of explants: In the spring of 2016, when the vegetative buds at the circumcision of the trigeminal bitter plants treated in step (1) were formed, the vegetative buds were cut from the base with a sterile scalpel and brought back in time after simple moisturizing and water-retaining treatment laboratory.

[0039] (3) Adventitious bud induction: the explants taken back to the laboratory were first rinsed with tap water overnight, and then sterilized in 75% ethanol solution in an ultra-clean workbench for 30 seconds, rinsed with sterile water for 3 times and sucked with sterile filter paper. After drying the water, put it in 0.1% mercuric chloride solution fo...

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Abstract

The invention relates to a plant tissue culture method in an agricultural biotechnology, and specifically, relates to a rapid propagation method of medicinal and edible homologous plant melicope pteleifolia(champion ex bentham)hartley in south of the Five Ridges, wherein the method comprises the following steps successively: obtaining of melicope pteleifolia(champion ex bentham)hartley plant explants, adventitious bud induction, adventitious bud proliferation culture, adventitious bud rooting culture, and test-tube plantlet transplanting. An adventitious bud induction culture medium of the adventitious bud induction includes a Nitsch culture medium, 1.0-3.0 mg / L 6-BA, 0.5-1.0 mg / L NAA, 0.01-0.05 mg / L TDZ, 25-30 g / L sucrose, 3.5-6.0 g / L agar, and 0.1-0.3 g / L activated carbon, wherein the pH is 5.4-5.8. The transplanting survival rate of melicope pteleifolia(champion ex bentham)hartley test-tube plantlets of the method is more than or equal to 95%.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of a Lingnan medicinal and edible homologous plant, trigeminus. Background technique [0002] Sanchaku (Melicope pteleifolia (Champion ex Bentham) Hartley), also known as Sanyaku, Sanyaku, Trident Tiger, Turtle Dove Flower, Three Guns, Trilegged Softshell Turtle, etc., is a medicinal plant of the Rutaceae Honey Cornus genus, which was first recorded in "Lingnan Herb Collection" is a commonly used Chinese herbal medicine in Lingnan area, which is often used to treat bruises, abscesses, eczema and other diseases. Sanchaku has the functions of clearing away heat and detoxification, expelling wind and dampness, reducing swelling and relieving pain, etc. Among the finished medicines, such as Sanjiu Ganmaoling Granules, Sanjiu Weitai, Sanjin Tablets, etc. At the same time, Sanchaku is a common medicinal and edible plant ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 梁钧淞杨业容莫昭展韦敏
Owner YULIN NORMAL UNIVERSITY
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