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Rapid propagation method for civil traditional Chinese herbal medicine desmodium triquetrum

A technology for cucurbit tea and Chinese herbal medicine, which is applied in the field of plant tissue culture, can solve the problems of no cucurbit tea tissue culturing technology, no application for a patent application for cucurbit tea tissue culture fast propagation, etc., and achieves the effect of solving the lack of seedlings

Inactive Publication Date: 2017-07-21
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the tissue culture technology of gourd tea at home and abroad, and there is no application for a patent for the tissue culture and rapid propagation of gourd tea

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Collection of explants: In the spring of 2014, new stem segments of vigorously growing Gourd Tea were selected as explants in the wild in Huanjiang County, Guangxi.

[0021] (2) Adventitious bud induction: the explants taken back to the laboratory were first rinsed with tap water overnight, then sterilized in 0.1% mercuric chloride solution in an ultra-clean workbench for 30 minutes, washed twice with sterile water, and then washed with sterile filter paper After drying the water, put it in 0.1% mercuric chloride solution for disinfection for 10 minutes. After rinsing with sterile water for 3 times, dry the water with sterile filter paper, and then sterilize it in 0.1% mercury solution for 3 minutes. After rinsing with sterile water for 5 times, dry it with sterile filter paper and cut it into 1.0-1.5 Adventitious buds can be induced by inoculating a segmented stem segment of about cm into adventitious bud induction medium at 25°C for 32 days in total darkness, with...

Embodiment 2

[0027] (1) Collection of explants: In the spring of 2015, new stem segments of vigorously growing gourd tea were selected as explants in the wild in Luocheng Mulao Autonomous County, Guangxi.

[0028] (2) Adventitious bud induction: the explants taken back to the laboratory were first rinsed with tap water overnight, and then sterilized in 0.1% mercuric chloride solution in an ultra-clean workbench for 45 minutes, rinsed with sterile water for 3 times, and then washed with sterile filter paper After drying the water, put it in 0.1% mercuric chloride solution for disinfection for 15 minutes. Rinse with sterile water for 4 times, dry the water with sterile filter paper, and then sterilize it in 0.1% mercuric chloride solution for 4 minutes. After rinsing with sterile water for 6 times, dry it with sterile filter paper and cut it into 1.0-1.5 Adventitious buds can be induced by inoculating a segmented stem segment of about cm into adventitious bud induction medium at 26°C for 39 ...

Embodiment 3

[0034] (1) Collection of explants: In the spring of 2016, the young stems of Gourd Tea growing robustly were selected as explants in the wild in Lijiang, Yunnan.

[0035](2) Adventitious bud induction: the explants taken back to the laboratory were first rinsed with tap water overnight, and then sterilized in 0.1% mercuric chloride solution in an ultra-clean workbench for 60 minutes, rinsed with sterile water for 3 times, and then washed with sterile filter paper After drying the water, put it in 0.1% mercuric chloride solution for disinfection for 20 minutes. After rinsing with sterile water for 5 times, dry the water with sterile filter paper, and then sterilize it in 0.1% mercury chloride solution for 5 minutes. After rinsing with sterile water for 7 times, dry it with sterile filter paper and cut it into 1.0-1.5 Adventitious buds can be induced by inoculating a segmented stem segment of about cm into adventitious bud induction medium at 28°C for 45 days in total darkness, ...

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Abstract

The invention relates to a method for plant tissue culture in an agricultural biological technique, and in particular to a rapid propagation method for a civil traditional Chinese herbal medicine, namely desmodium triquetrum. The rapid propagation method comprises the following steps in sequence: collecting explants of desmodium triquetrum, performing adventitious bud induction, performing subculture, performing rooting culture, and performing transplanting, wherein an adventitious bud culture medium for inducing adventitious buds comprises MS (Murashige-Skoog), 1.0-3.0mg / L 6-BA, 0.1-1.0mg / L NAA, 25-30g / L cane sugar and 3.5-6.0g / L agar; the pH value of the adventitious bud culture medium is 5.4-5.9. Due to a culture medium formula and culture conditions of adventitious bud induction screening and optimization, adventitious bud multiplication, rooting culture and the like, and through steps of induction, subculture, rooting, transplanting and the like, a very high induction rate is achieved, the induction rate is up to 89% or greater, rapid propagation of the desmodium triquetrum is achieved, and thus the purpose of the invention is achieved.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of folk Chinese herbal medicine gourd tea. Background technique [0002] Gourd tea (Tadehagi triquetrum (L.) Ohashi), also known as Cordyceps, Golden Sword Grass, Shrub Grass, Salmongrass, Bai Lao Tong, etc., is a semi-shrub plant in the leguminous family Tadehagi triquetrum. There are about 6 species of gourd tea, which are mainly distributed in tropical Asia, the Pacific Islands and Australia. Among them, Tadehagi triquetrum (L.) Ohashi and Tadehagi pseudatriquetrum (DC.) Yang et Huang are distributed in China. ), mainly distributed in Guangxi, Guangdong, Hainan and other South China regions. It is common in low mountains and Pingba roadside bushes, and it likes warm climate conditions. [0003] Gourd tea is a commonly used Chinese herbal medicine in the folk. It has the functions of clearing heat and detoxifying,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁钧淞莫昭展杨业容韦敏
Owner YULIN NORMAL UNIVERSITY
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