Tissue culture rapid propagation method of ornamental lily

A tissue culture and lily technology is applied in the field of tissue culture and rapid propagation of ornamental lilies, which can solve the problems affecting the large-scale production and application of lily flowers, degenerate interspecific hybridization, and small reproduction coefficient, and avoid interspecific hybridization incompatibility, The effect of promoting production and increasing the reproduction coefficient

Active Publication Date: 2012-04-11
珠海慧洁生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional lily propagation methods, such as dividing balls, dividing bulbils or scale cuttings, scale embedding, etc., have problems such as small reproduction coefficient, multi-generation reproduction often causes degradation and interspecific hybridizatio

Method used

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  • Tissue culture rapid propagation method of ornamental lily
  • Tissue culture rapid propagation method of ornamental lily

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Select the non-damaged scales in the middle layer of Mona lily, clean them with clean water, then treat them with 75% alcohol for 5 seconds, then treat them with 0.1% mercuric chloride for 15 minutes, and then rinse them with sterile water for 5 times;

[0017] (2) Cut the sterilized scales into 1cm 2 The small pieces were inoculated on MS with different concentrations of NAA and 6-BA induction medium, and induced to bud out for 25 days under the induction conditions of temperature 25°C, light intensity 2000lx, light cycle 12 hours / day, and the induction results were as follows: As shown in Table 1;

[0018] Table 1

[0019] Culture medium number

NAA(mg / L)

6-BA(mg / L)

Induction rate (%)

1

0.5

0.1

42.8

2

0.5

0.2

63.4

3

0.5

0.5

78.6

4

1

0.1

67.3

5

1

0.2

84.6

6

1

0.5

96.5

7

...

Embodiment 2

[0026] (1) Select the undamaged scales in the middle layer of Lilium barbara, clean them with clear water, then treat them with 75% alcohol for 10 seconds, then treat them with 0.1% mercury liter for 5 minutes, and then rinse them with sterile water for 7 times;

[0027] (2) Cut the sterilized scales into 2cm 2 The small pieces were inoculated on MS with different concentrations of NAA and 6-BA induction medium, and induced to bud out for 27 days under the induction conditions of temperature 23°C, light intensity 2000lx, light cycle 12 hours / day, and the induction results were as follows: Table 3 shows:

[0028] table 3

[0029] Culture medium number

NAA(mg / L)

6-BA(mg / L)

Induction rate (%)

1

0.5

0.1

43.2

2

0.5

0.2

62.8

3

0.5

0.5

78.2

4

1

0.1

67.8

5

1

0.2

84.1

6

1

0.5

96.8

7

2

...

Embodiment 3

[0036] (1) Select the undamaged scales in the middle layer of Lily chrysanthemum, wash them with clean water, then treat them with 75% alcohol for 10 seconds, then treat them with 0.1% mercuric chloride for 15 minutes, and then rinse them with sterile water for 7 times;

[0037] (2) Cut the sterilized scales into 1cm 2 Small pieces, inoculated on MS+NAA 1.0mg / L+6-BA0.5mg / L induction medium, under the induction conditions of temperature 27°C, light intensity 2000lx, and light cycle 12 hours / day, induce 23 days to sprout and become bulbs clump,

[0038] (3) Cut 3 single buds from the induced bulb clusters and transfer them to MS+6-BA 1.0mg / L+NAA0.5mg / L proliferation medium, at a temperature of 27°C, a light intensity of 2000lx, and a light cycle of 12 hours / Proliferation was carried out under the condition of 30 days for 30 days, the number of inoculations was 30, and the total number of buds after proliferation was 318;

[0039] (4) Select cluster buds with a leaf height of ...

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Abstract

The invention discloses a tissue culture rapid propagation method of an ornamental lily The method comprises the following steps: selecting a scale without injuries at an intermediate layer of the ornamental lily, washing and cleaning the scale with clear water, sequentially processing the cleaned scale with 75% alcohol and 0.1% mercuric chloride, and then washing with sterile water for 5-7 times; cutting the scale which is well sterilized into small pieces with the area of 1-2cm<2>, inoculating the small pieces onto an induction culture medium, performing induction for 23-27 days under induction conditions, and budding and becoming a patch of bulb; cutting 2-3 single buds from the induced patch of bulb, and transferring to a propagation culture medium for performing propagation culture for 25-35 days; selecting the clumpy buds with the blade height of 2-4cm from the propagated patch of bulb, cutting into the single buds and inoculating the single buds onto a rooting culture medium for culturing and rooting for 20-26 days so as to become test-tube plantlets; and performing acclimatization on the test-tube plantlets, then cleaning, and transplanting into a nutrient matrix formed by mixing yellow soil with vermiculite in a volume ratio of 1: 1 for cultivation and management. By adopting the method, the propagation coefficient is increased, the propagation speed is accelerated, the effects of detoxification and rejuvenation are achieved, and the problems of degradation, incompatibility of interspecific hybridization and the like, which are often caused by multi-generation propagation, can be avoided.

Description

【Technical field】 [0001] The invention relates to a tissue culture rapid propagation method for ornamental lilies, belonging to the technical field of plant tissue culture. 【Background technique】 [0002] Lilies are important cut flowers, potted plants and garden plant material. The traditional methods of lily propagation, such as dividing bulbs, dividing bulbils or scale cuttings, and embedding scales, have problems such as small reproductive coefficients, multi-generational propagation often causing degradation, and interspecific hybridization incompatibility. Relying on the traditional asexual reproduction method, the virus is passed and accumulated from generation to generation, and the harm is becoming more and more serious, which affects the large-scale production and application of lily flowers. 【Content of invention】 [0003] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a method for rapid propagation of ornament...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨懋勋陈考科单振菊陈志云蒋建平吴旖谢婉
Owner 珠海慧洁生物有限公司
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