Development and applications of Indel marker capable of distinguishing five pepper cultivated species

A technology of cultivars and molecular markers, applied in the field of agricultural biotechnology engineering, can solve the problems of low repeatability and easy to be affected by the environment, and achieve the effects of saving manpower, convenient operation, and simple belt type

Active Publication Date: 2018-09-28
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Although the morphological method is simple and intuitive, it is easily affected by the environment and the reproducibility is not high

Method used

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  • Development and applications of Indel marker capable of distinguishing five pepper cultivated species
  • Development and applications of Indel marker capable of distinguishing five pepper cultivated species
  • Development and applications of Indel marker capable of distinguishing five pepper cultivated species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Test materials

[0021] The two re-sequenced materials include: high-generation inbred line material G29, belonging to Capsicum annuum, from the Vegetable Research Institute of Jiangsu Academy of Agricultural Sciences; PBC688, belonging to Capsicum frutescens, introduced from the Asian Vegetable Center. The 58 pepper materials used to verify the accuracy of the markers are all from the National Plant Germplasm System (NPGS) and belong to 5 pepper cultivars, of which the serial numbers 1 and 9 are 2 homozygous isolates from PI 194881 Lines, 26, 27 and 28 are 3 homozygous lines isolated from PI 241668; see Table 1 for details.

[0022] Table 1. 58 materials used to verify the accuracy of labeling

[0023]

[0024]

[0025] 2. Indel mark development

[0026] Use the resequencing data to compare with the reference genome to find InDel sites. The version of the reference genome is pepper.V.1.55, and the download URL is http: / / peppergenome.snu.ac.kr / download.php. ...

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Abstract

The invention discloses development and applications of an Indel marker capable of distinguishing five pepper cultivated species. An Indel marker primer capable of distinguishing five pepper cultivated species is composed of a forward primer as shown in SEQ ID NO.1 and a reverse primer as shown in SEQ ID NO.2. The genome DNA of the five pepper cultivated species materials is subjected to PCR amplification by adopting the primer provided by the invention, the five pepper cultivated species can be rapidly and accurately distinguished, the five pepper cultivated species can be distinguished, andthe inter-species hybrid variety of the five cultivated species can be identified.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology engineering and relates to the development and application of an Indel marker for distinguishing five pepper cultivars. Background technique [0002] Capsicum belongs to the genus Capsicum of the family Solanaceae. For quite a long time, there were no specific and standard classification rules for peppers, which brought many obstacles to international exchanges, international breeding and sharing of germplasm resources. In 1753, Linne first divided peppers into two categories: annual peppers (Capsicum annuum L.) and shrubby peppers (Capsicum frutescens L.). In 1898, Irish divided annual peppers into 7 varieties based on the Linne classification: Var.longum (D.C) Sendt, Var.conoides (Mill) Irish, Var.fasciculatum (sturt) Irish, Linear pepper Var.acuminatum Fingern, round pepper Var.grossumL.Sendt, cone pepper Var.abberviatum Fingern, cherry pepper Var.cerasiforme (Mill) Irish. In 1923, B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/16
Inventor 郭广君王述彬潘宝贵刁卫平戈伟刘金兵高长洲
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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