Somatic embryogenesis and plant regeneration technology for hybridized Chinese tuliptree

A technology of hybrid tulip tree and embryogenesis, applied in plant regeneration, botanical equipment and methods, horticulture, etc., can solve the problems that have not yet been published with a complete set of technology for hybrid tulip tree cells and embryos

Inactive Publication Date: 2002-10-23
NANJING FORESTRY UNIV
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  • Abstract
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Problems solved by technology

[0010] 2. The explants must be refrigerated at low temperature (4-8°C) for a period of time before inoculation. For example, gymnosperms need to be refrigerated for 1-2 months, and they cannot be started without low-temperature treatment.
[0030] In all kinds of literatures about plant tissue culture technology, there is no report on the somatic embryogenesis of Liriondendron hybrid (Liriondendron hybrid) except the report on the tissue culture-related patent technology of Liriondendron tulipifera L. in the United States. Complete technology release

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  • Somatic embryogenesis and plant regeneration technology for hybridized Chinese tuliptree
  • Somatic embryogenesis and plant regeneration technology for hybridized Chinese tuliptree
  • Somatic embryogenesis and plant regeneration technology for hybridized Chinese tuliptree

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Embodiment Construction

[0123] The following are typical embodiments of the invention:

[0124] 1. Use immature zygotic embryos to induce embryogenic callus. Artificially pollinate in spring, collect aggregated samara in midsummer, refrigerate the collected aggregated samara at 4°C for 4 to 6 days, cut open and cut off the wings. Wash the seeds with detergent to remove the oil on the surface, rinse with tap water for 30 minutes, rinse with distilled water for 3 times, process with 75% ethanol for 20 seconds, use 0.1% mercuric chloride for 4 minutes and 30 seconds, and rinse with sterile water for 4 times. Under sterile conditions, the seed coat was peeled off, and the immature embryos were inserted into the induction medium.

[0125] 1 / 2MS medium was used in the stage of embryogenic callus induction, and the concentrations of additional hormones were 2, 4-D 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 5.0 mg / L, 6-BA 0, 0.1, 0.2, 0.4, 0.5mg / L. Add 500-1000mg / L natural compound hydrolyzed casein. Vitamin C 5mg / L,...

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Abstract

The invention is characterized by that on the basis of choice of parents of basswood generic cross and breeding of interspecfic hybridization, the immature embryo of limited quantities got respectively from normal, reciprocal an rotation cross is engaged in induction and cultivation of embryo somatric cell, produces large quantities of embryo of somatic cell in synchronizing development, and leads to cultivate embryo of somatic cell into whole tree characteristics.

Description

1. Technical field [0001] The invention belongs to the technical field of cell engineering seedling breeding in the forestry industry. 2. Background technology [0002] Liriodendron, also known as mandarin wood, duck foot wood, nine-layer bark, maple lotus, etc., is a plant of the genus Liriondendron in the family Magnoliaceae. There are only two species of this genus, one is the Chinese tulipwood (L. .), and the other is Liriodendron tulipifera (L.tulipifera L.) distributed in the eastern United States. Liriodendron genus occupies a relatively primitive position in the taxonomic status of angiosperms. Although the distribution area is wide, it is mostly distributed in small groups at present. Liriodendron, which is distributed in China, has been listed as a national second-class rare and endangered tree species. [0003] Liriodendron chinensis has a straight trunk and beautiful tree shape; the leaf shape is peculiar, wide and slightly concave, with a lobes on both sides...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 施季森陈金慧诸葛强黄敏仁
Owner NANJING FORESTRY UNIV
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