Streptomyces albidoflavus and application thereof in apple tree rot prevention and treatment

Abstract

The invention discloses streptomyces albidoflavus Actin-1. The streptomyces albidoflavus Actin-1 can grow and propagate with apple tree rot pathogenic bacterium mycelium as nutrition, long-term colonization of biocontrol bacteria on apple tree rot scabs is promoted, a long-term biological prevention and treatment function is achieved, meanwhile, the streptomyces albidoflavus Actin-1 is induced to generate multiple ectoenzyme cell wall hydrolytic enzymes, and pathogenic bacterium cells disintegrate in combination with the enzyme dissolving function; high antibacterial active matter can be generated, the bacteriostasis rate on the apple rot pathogenic bacteria is 89.82%, a good antibacterial effect is achieved on botryosphaeria berengeriana and other pathogenic bacteria, the bacteriostasis rate ranges from 76.08% to 87.10%, and broad spectrum bacteriostasis performance is achieved. The streptomyces albidoflavus Actin-1 is adopted as main biocontrol bacteria for preventing and treating the apple tree rot and other fruit and vegetable pathogenic bacteria, and the advantages of being good in prevention and treatment effect (100%), high in efficiency, low in recurrence rate (0), high in environment adaption capacity, high in stability, not likely to generate resistance to drugs and the like are achieved. Important significance is achieved on improving the prevention and treatment effect on the apple tree rot and other fruit and vegetable pathogenic bacteria, preventing pathogenic bacterium relapse and protecting the environment.

Description

technical field [0001] The invention relates to biocontrol bacteria, in particular to a strain of Streptomyces albus and its application in the prevention and treatment of apple tree rot. Background technique [0002] Apple tree rot (Valsa mali Miyabe et Yamada), commonly known as rotten skin disease and stinky skin disease, is an important disease of northern apple trees caused by the genus Valsa ceratosperma. The disease mainly harms fruiting trees that are more than 3 years old, causing tree vigor to weaken, branches to die, dead trees and even garden destruction. The symptoms of the disease are mainly ulcer-based. In the ulcer type, reddish-brown, water-stained, slightly raised, round to oblong lesions appear on the trunk and bark in early spring. The texture is soft, easy to tear, and easy to sag when pressed by hand. In the later stage, it shrinks and sinks, and there are cracks on the edge of the diseased part. There are obvious small black spots, that is, pycnidia....

AI Technical Summary

Problems solved by technology

[0022] The technical problem solved by the present invention is to overcome the defect of the existing anti-bacteria of apple tree rot disease—Streptomyces disease prevention mechanism, take the microbiological characteristics of Streptomyces as the research object, and take the pathological characteristics and nutritional structure characteristics of the pathogenic bacteria of apple tree rot disease And the metabolic characteristics of extracellular hydrolase as the screening target, isolate and screen a strain that can directly use the mycelia of the pathogenic fungus of apple tree rot as nutrition for growth and reproduction, and at the same time induce itself to produce a variety of extracellular hydrolysis that can degrade the cell wall of the pathogenic bacteria Enzyme, long-term colonization in apple tree rot disease spots to improve disease prevention effect and prevent streptomyces from recurrence of apple tree rot disease

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