The invention discloses a crop physiology resistance inducer and a preparation method. The crop physiology resistance inducer is prepared from nano SiO2, nano CaCO3, heteroauxin, abscisic acid, humic acid, monopotassium phosphate and distilled water according to certain ratio. The preparation method comprises the following steps: (1) taking nano SiO2, nano CaCO3, heteroauxin, abscisic acid, humic acid, monopotassium phosphate and distilled water for standby application; (2) weighing the raw materials respectively according to the mass percent of the preparation amount, dissolving nano SiO2 and nano CaCO3 with ethyl alcohol respectively, mixing the solutions, after certain period of time, adding heteroauxin and abscisic acid, adding distilled water for dissolution, uniformly stirring, performing ultrasonic treatment, adding humic acid and monopotassium phosphate, adjusting the pH value with hydrochloric acid, and adding distilled water for constant volume to obtain the crop physiology resistance inducer. According to the invention, the formula is reasonable, the application is convenient, the resistance of crops to drought, low temperature, hard light and other adverse situations can be effectively induced, and high and stable yield of the crops can be ensured; the preparation method is practical, simple and convenient to operate, stable in ingredient dispersion, ecological and environment-friendly, free of environmental pollution, and convenient to popularize and apply.