The invention belongs to the technical field of skin care products, and provides an anti-wrinkle eye scream containing NMN and a preparation method and application thereof. The anti-wrinkle eye creamis prepared from allantoin, glycerinum, carbomer, polyacrylamide, butanediol, polydimethylsiloxane, squalane, lauryl alcohol polyether-7, cetearyl alcohol, hydrolyzed jojoba esters, glyceryl stearate,PEG-100 stearate, tocopheryl acetate, pentanediol, triethanolamine, p-hydroxyacetophenone, pigments, propylene glycol, lipoic acid, nicotinamide mononucleotide, plant extracts, lactobacillus ferments, fucoidin, ethylhexylglycerin, 1,2-hexanediol, dipeptide diaminobutyryl benzylamide diacetate, whey protein, polypeptide, hydrolyzed protein and purified water. The effective ingredients in the anti-wrinkle eye cream can quickly penetrate the skin, the muscle cell contraction is effectively reduced, the collagen is supplemented, the synthesis of skin protein and hyaluronic acid is promoted, and the effects of tightening skin and reducing wrinkles are achieved.