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Fermented liquid of biocontrol bacteria for preventing and treating fruit tree rot and preparation method thereof

A technology for fruit tree rot disease and fermentation broth, which is applied in the field of fruit tree disease and insect pest control, and can solve the problems of narrow antibacterial spectrum, single application range and low control effect.

Active Publication Date: 2020-10-20
SHIJIAZHUANG POMOLOGY INST OF HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the widely used biocontrol bacteria mainly include Bacillus, Pseudomonas, and Agrobacterium radiobacter, etc. The antagonism between the strains is strong, the antibacterial spectrum is narrow, and the control effect is low. There are also many deficiencies in the existing products in terms of industrial application, environmental adaptability, and stress resistance, which limit the use of biocontrol bacteria in the aspect of fruit tree rot. Therefore, it is extremely necessary to develop new biocontrol bacteria so that it can meet the needs of fruit tree rot disease control

Method used

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  • Fermented liquid of biocontrol bacteria for preventing and treating fruit tree rot and preparation method thereof
  • Fermented liquid of biocontrol bacteria for preventing and treating fruit tree rot and preparation method thereof
  • Fermented liquid of biocontrol bacteria for preventing and treating fruit tree rot and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A fermented liquid of bio-control bacteria for preventing and treating fruit tree rot, which is prepared by mixing the fermented liquid of Metschnifera Y-3 and fermented liquid of Trichoderma longibranchus CD-6 according to the mass ratio of 1:8;

[0028] Among them, the total number of colonies in the fermentation broth of biocontrol bacteria is 1.0×10 8 cfu / mL.

[0029] Include the following steps:

[0030] Step 1. Streak culture the pure strain of Metschnijs Y-3 on a PDA plate, culture at 28°C for 48 hours, then pick a single colony and culture it in NYDB liquid medium at a shaking speed of 200r / min for 72 hours to obtain Metschneri yeast The initial fermentation broth of Y-3;

[0031] Streak culture the pure strain of Trichoderma longibrachiata CD-6 on a PDA plate, culture at 28°C for 48 hours, pick a single colony and culture it in YSP liquid medium at a shaking speed of 200r / min for 24 hours to obtain Trichoderma longibrachiata CD-6 6 initial fermented liquid; ...

Embodiment 2

[0036] A fermented liquid of bio-control bacteria for preventing and treating fruit tree rot, which is prepared by mixing the fermented liquid of Metschnifera Y-3 and fermented liquid of Trichoderma longibranchus CD-6 according to the mass ratio of 1:5;

[0037] Among them, the total number of colonies in the fermentation broth of biocontrol bacteria is 1.0×10 6 cfu / mL.

[0038] The preparation method is the same as in Example 1, except that the formula in Example 1 is replaced with that of Example 2.

Embodiment 3

[0040] A fermented liquid of biocontrol bacteria for preventing and treating fruit tree rot, which is prepared by mixing the fermented liquid of Metschnifera Y-3 and fermented liquid of Trichoderma longibranchus CD-6 according to the mass ratio of 1:10;

[0041] Among them, the total number of colonies in the fermentation broth of biocontrol bacteria is 1.0×10 10 cfu / mL.

[0042] The preparation method is the same as in Example 1, except that the formula in Example 1 is replaced with that of Example 3.

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Abstract

The invention discloses biocontrol bacterium fermentation liquid for preventing and curing fruit tree rotten disease. The biocontrol bacterium fermentation liquid is prepared from metschnikowia Y-3 fermentation liquid and trichoderma longibrachiatum rifai CD-6 fermentation liquid according to the mass ratio of 1:(5 to 10), wherein the total number of bacterial colonies in the biocontrol bacteriumfermentation liquid is 1.0*10<6> to 1.0*10<10>cfu / mL. In addition, the invention further provides a preparation method of the biocontrol bacterium fermentation liquid for preventing and curing the fruit tree rotten disease. The biocontrol bacterium fermentation liquid has the multiple advantages of being good in preventing and cure effect, high in efficiency, low in recurrence rate, high in adaptive capacity to environment, high in stability and not prone to generating resistance to drugs, is the preferred choice of biological prevention and control, and has important meanings on improving theprevention and cure effects on fruit and vegetable pathogenic bacteria such as fruit tree rotten disease, preventing pathogenic bacteria from recurring, and protecting environment.

Description

technical field [0001] The invention belongs to the technical field of prevention and control of fruit tree diseases and insect pests, and in particular relates to a fermentation liquid of biocontrol bacteria for preventing and treating fruit tree rot and a preparation method thereof. Background technique [0002] Rot is a major branch disease of fruit trees that occurs all over the world. For many years, rot disease has spread in a large number in various places. It is the first-class disease that endangers my country's fruit forest industry, and is called the cancer of fruit trees. Fruit tree rot is mainly caused by fungi, which are weak parasites and invade mainly through wounds, and can also invade through lenticels and bud eyes of branches. For trees with strong growth and strong disease resistance, the bacteria can lie dormant for a long time without developing disease; while for trees with weak tree vigor and poor disease resistance, the disease occurs quickly, causi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/32A01N63/38A01P3/00C12N1/14C12N1/16C12R1/645C12R1/885
CPCA01N63/30C12N1/14C12N1/16
Inventor 马文会杨阳李学华王景涛贾彦丽王越辉白瑞霞吴硕武晓红董辉
Owner SHIJIAZHUANG POMOLOGY INST OF HEBEI ACADEMY OF AGRI & FORESTRY SCI
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