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A strain of Streptomyces albicans and its application in the control of apple tree rot

The invention relates to a technology for Streptomyces whitish and apple tree rot disease, which is applied to Streptomyces whitish and its application field in the prevention and control of apple tree rot disease, and achieves the effects of good bacteriostatic effect, strong weather resistance and maintaining strain vitality.

Active Publication Date: 2019-08-20
陕西枫丹百丽生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] The technical problem solved by the present invention is to overcome the defect of the existing anti-bacteria of apple tree rot disease—Streptomyces disease prevention mechanism, take the microbiological characteristics of Streptomyces as the research object, and take the pathological characteristics and nutritional structure characteristics of the pathogenic bacteria of apple tree rot disease And the metabolic characteristics of extracellular hydrolase as the screening target, isolate and screen a strain that can directly use the mycelia of the pathogenic fungus of apple tree rot as nutrition for growth and reproduction, and at the same time induce itself to produce a variety of extracellular hydrolysis that can degrade the cell wall of the pathogenic bacteria Enzyme, long-term colonization in apple tree rot disease spots to improve disease prevention effect and prevent streptomyces from recurrence of apple tree rot disease

Method used

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  • A strain of Streptomyces albicans and its application in the control of apple tree rot
  • A strain of Streptomyces albicans and its application in the control of apple tree rot
  • A strain of Streptomyces albicans and its application in the control of apple tree rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Isolation, screening and identification of Streptomyces albidoflavus Actin-1

[0059] 1) Separation and primary screening: Accurately weigh 10g of the orchard soil sample in Yantai City, Shandong Province, add it to a sterilized triangular flask with glass beads containing 100mL of cooled sterile water, shake and mix at 200r / min for 30min, and let stand for 5min Aspirate 1mL of the supernatant, and serially dilute to obtain 10 -3 ~10 -9 Concentration, select 10 -3 、10 -4 、10 -5 、10 -6 、10 -7 Use a pipette to draw 100 μL of each gradient suspension, spread it evenly on the screening medium plate with a spreader, and use sterile water as a control, place the plate upside down in the incubator, and incubate at 30°C for 7 days. Each treatment setting was repeated horizontally for 3 groups. According to the size of the transparent circle and the characteristics of the shape, size, sporulation, surface structure, edge structure, texture, color or soluble pigme...

Embodiment 2

[0127] Embodiment 2 Streptomyces albicans Actin-1 growth temperature and acid and alkali resistance test

[0128] Preparation of spore suspension of Streptomyces microalbubus Actin-1: inoculate Streptomyces microalbusus Actin-1 on the slant of Gao's No. 1 agar medium by streaking, culture at 30°C for 7 days until spore production; add 5 mL of sterile water, and incinerate Scrape the spores into sterile water with the bacterial inoculation needle, shake and mix well, and adjust the spore concentration to 1×10 6 cells / mL to obtain a spore suspension.

[0129] 1. Growth temperature test

[0130] Prepare 2216E liquid medium, pack in 48 500mL Erlenmeyer flasks, 100mL / bottle, sterilize at 121°C for 30min, and set aside; inoculate the above spore suspension into 36 Erlenmeyer flasks according to 5‰ (V / V) , as the treatment group, 12 Erlenmeyer flasks without inoculation as the control group, according to 0 ℃, 5 ℃, 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃, 45 ℃, 50 ℃, 55°C temperat...

Embodiment 3

[0138] Embodiment 3 Streptomyces albicans Actin-1 broad-spectrum antibacterial verification and genetic stability test:

[0139] Carry out the bacteriostasis experiment of 11 strains of pathogenic bacteria such as the pathogenic bacteria of apple tree rot disease (apple black rot skin fungus) and the pathogenic bacteria of apple spot leaf fall disease (Alternaria apple specialization type) by Streptomyces albus Actin-1, the specific operation steps are as follows:

[0140] 1) Inoculate Streptomyces albus Actin-1 on Gaoshi No. 1 agar medium plate by streaking, and cultivate it at 30° C. for 5 days; spot the pathogenic bacteria of apple tree rot disease on the PDA medium plate, and cultivate it at 28° C. for 7 days; Obtain activated Streptomyces albicans Actin-1 and activated pathogenic bacteria;

[0141] 2) Confrontation experiment 2: draw a cross on the back of the PDA medium plate with a diameter of 90 mm, take the point of the cross as the center of the plate, and inoculate ...

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Abstract

The invention discloses a Streptomyces albicans Actin-1, which can grow and reproduce with the mycelium of the pathogenic fungus of apple tree rot disease as nutrition, which is beneficial to the long-term colonization of the biocontrol bacteria on the rot spots of apple trees, and exerts long-term biological control At the same time, it induces itself to produce a variety of extracellular cell wall hydrolytic enzymes, disintegrates the pathogenic bacteria cells through the synergistic enzymolysis, and can produce strong antibacterial active substances. Various pathogenic bacteria such as Botrytis berenger have good antibacterial effect, the antibacterial rate is 76.08‑87.10%, and they have broad-spectrum antibacterial properties; this fungus is used as the main biocontrol fungus to prevent and control apple tree rot and other fruit and vegetable pathogenic bacteria have multiple advantages such as good control effect (100%), high efficiency, low recurrence rate (0), strong adaptability to the environment, strong stability, and not easy to produce drug resistance. It is of great significance to improve the effect, prevent the recurrence of pathogenic bacteria, and protect the environment.

Description

technical field [0001] The invention relates to biocontrol bacteria, in particular to a strain of Streptomyces albus and its application in the prevention and treatment of apple tree rot. Background technique [0002] Apple tree rot (Valsa mali Miyabe et Yamada), commonly known as rotten skin disease and stinky skin disease, is an important disease of northern apple trees caused by the genus Valsa ceratosperma. The disease mainly harms fruiting trees that are more than 3 years old, causing tree vigor to weaken, branches to die, dead trees and even garden destruction. The symptoms of the disease are mainly ulcer-based. In the ulcer type, reddish-brown, water-stained, slightly raised, round to oblong lesions appear on the trunk and bark in early spring. The texture is soft, easy to tear, and easy to sag when pressed by hand. In the later stage, it shrinks and sinks, and there are cracks on the edge of the diseased part. There are obvious small black spots, that is, pycnidia....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/205C12R2001/465
Inventor 李玲娣王灵敏孙振娜严立恩李学辉周天慧王江王楠
Owner 陕西枫丹百丽生物科技有限公司
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