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Preparation method of apple tree canker antibiological inoculant

A technology of apple tree rot and biocontrol fungicide, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the problem of incomplete disease prevention, inability to cure apple tree rot, Streptomyces disease prevention effect is not very ideal and other problems, to achieve the effect of improving biocontrol effect, good strain vitality, and maintaining antibacterial effect

Active Publication Date: 2017-06-30
陕西枫丹百丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] The disease prevention effect of Streptomyces disclosed above is still not very satisfactory, and the disease prevention mechanism is the same as most bacteria and fungi, mainly through the metabolism to produce antibiotics, antimicrobial peptides and other antibacterial substances to produce antagonistic effects to inhibit the growth and reproduction of pathogenic bacteria, and the disease prevention effect Incomplete, can not cure apple tree rot

Method used

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  • Preparation method of apple tree canker antibiological inoculant
  • Preparation method of apple tree canker antibiological inoculant
  • Preparation method of apple tree canker antibiological inoculant

Examples

Experimental program
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Effect test

Embodiment 1

[0058] A preparation method of an apple tree rot biocontrol fungicide, comprising the steps of:

[0059] 1) Preparation of fermented seed liquid: Inoculate Streptomyces microalbubus Actin-1 strain on the slant of Gaoshi No. 1 agar medium by streaking, culture at 30°C for 7 days until sporulation; then add 5 mL of sterile water to the slant, and use Scrape off the spores with a sterilized inoculation needle, add sterile water, shake and mix to obtain a spore suspension; inoculate the spore suspension into a liquid seed medium according to the inoculum size 1% (v / v), 30°C, 200r / min, the shake flask cultured for 48h to obtain the fermented seed liquid;

[0060] 2) Preparation of solid fermentation medium: according to the formula of solid fermentation medium, K 2 HPO 4 , MgSO 4 ·7H 2 Dissolve O, then mix with other raw materials, pack into sterilized bags, sterilize at 121°C for 45 minutes, cool to 35°C; lay flat on a 70cm long, 50cm wide, and 5cm deep sterilized curved plat...

Embodiment 2

[0074] A preparation method of an apple tree rot biocontrol fungicide, comprising the steps of:

[0075] 1) Preparation of fermented seed liquid: Inoculate Streptomyces microalbubus Actin-1 strain on the slant of Gaoshi No. 1 agar medium by streaking, culture at 28°C for 6 days until sporulation; then add 5 mL of sterile water to the slant, and use Scrape off the spores with a sterilized inoculation needle, add sterile water, shake and mix to obtain a spore suspension; inoculate the spore suspension into the liquid seed medium according to the inoculum size 0.8% (v / v), at 28°C, 180r / min, the shake flask cultured for 46h to obtain the fermented seed liquid;

[0076] 2) Preparation of solid fermentation medium: according to the formula of solid fermentation medium, K 2 HPO 4 , MgSO 4 ·7H 2 Dissolve O, then mix with other raw materials, pack into sterilized bags, sterilize at 121°C for 45 minutes, cool to 35°C; lay flat on a 70cm long, 50cm wide, and 5cm deep sterilized curv...

Embodiment 3

[0090] A preparation method of an apple tree rot biocontrol fungicide, comprising the steps of:

[0091] 1) Preparation of fermented seed liquid: inoculate Streptomyces microalb. Scrape off the spores with a bacterial inoculation needle, add sterile water, shake and mix to obtain a spore suspension; inoculate the spore suspension into the liquid seed medium according to the inoculum size 1.2% (v / v), 32°C, 220r / m min, shake the flask for 50 hours to obtain the fermented seed liquid;

[0092] 2) Preparation of solid fermentation medium: according to the formula of solid fermentation medium, K 2 HPO 4 , MgSO 4 ·7H 2 Dissolve O, then mix with other raw materials, pack into sterilized bags, sterilize at 121°C for 45 minutes, cool to 35°C; lay flat on a 70cm long, 50cm wide, and 5cm deep sterilized curved plate;

[0093] 3) solid fermentation: step 1) fermented seed solution is inoculated into step 2) in the curved plate that fills solid fermentation medium according to the ino...

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Abstract

The invention discloses a preparation method of an apple tree canker antibiological inoculant. The method comprises the steps that apple tree canker pathogen hyphae can be directly adopted as nutrition for growth and breeding, apple tree canker pathogen hyphae can be induced to produce various exocellular enzymes for degrading pathogen cell walls, streptomyces albidoflavus Actin-1 colonized at apple tree canker scab for a long time is activated to produce spores, a spore suspension is prepared, then, the spore suspension is inoculated into a liquid seed culture medium to be fermented to obtain a fermentation seed solution, finally, the fermentation seed solution is inoculated into a solid fermentation culture medium for solid fermentation to obtain spore raw powder, and then, the spore raw powder, an ultraviolet protectant, a dispersing agent, a carrier and other auxiliaries are scientifically screened and compounded to obtain the antibiological inoculant with the multiple advantages of being good in control efficiency, high in efficiency, low in recurrence rate, high in environment adaptability, high in stability, not likely to generate drug resistance and the like; the antibiological inoculant is the first choice for biological control, and is of great significance in improving the control effect of garden stuff pathogenic bacteria such as apple tree canker, preventing pathogenic bacterium relapse and achieving environmental protection.

Description

technical field [0001] The invention relates to the preparation of a biocontrol bacterium, in particular to a preparation method of a biocontrol bacterium for apple tree rot disease. Background technique [0002] Apple tree rot (Valsa mali Miyabe et Yamada), commonly known as rotten skin disease and stinky skin disease, is an important disease of northern apple trees caused by the genus Valsa ceratosperma. The disease mainly harms fruiting trees that are more than 3 years old, causing tree vigor to weaken, branches to die, dead trees and even garden destruction. The symptoms of the disease are mainly ulcer-based. In the ulcer type, reddish-brown, water-stained, slightly raised, round to oblong lesions appear on the trunk and bark in early spring. The texture is soft, easy to tear, and easy to sag when pressed by hand. In the later stage, it shrinks and sinks, and there are cracks on the edge of the diseased part. There are obvious small black spots, that is, pycnidia. When...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
Inventor 李玲娣王灵敏孙振娜严立恩李学辉王江王楠
Owner 陕西枫丹百丽生物科技有限公司
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