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Bacterial strain for producing prodigiosin and method thereof

A technology for the production of prodigiosin and prodigiosin, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low overall yield and large difference in prodigiosin synthesis ability, etc. Achieve high production efficiency, simple operation, and good genetic stability

Inactive Publication Date: 2011-04-06
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prodigiosin-producing bacteria found so far are mainly concentrated in different strains of Serratia marcescens, including Serratia marcescens Nima, Serratia marcescens R-2, Serratia marcescens W 0206B-20, Serratia marcescens RZ21, but the strains are different, bleeding bacteria The synthetic ability of red pigment varies greatly, and the overall yield is low

Method used

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  • Bacterial strain for producing prodigiosin and method thereof
  • Bacterial strain for producing prodigiosin and method thereof
  • Bacterial strain for producing prodigiosin and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 prodigiosin

[0033] Streak culture of Serratia marcescens strains (CGMCC 4074) on LB solid medium at 25-40°C for constant temperature, and then store at 2-4°C for a short period of time: the composition of the solid medium, In terms of weight and volume percentage, the unit is g / 100ml, including 1.0% peptone, 0.5% yeast powder, 1.0% NaCl, 2.0% agar powder, and a pH value of 6.5.

[0034] Insert the above-mentioned activated single bacterium colony into a 250ml Erlenmeyer flask equipped with a liquid medium, with a liquid volume of 50ml / 250ml shake flask, and cultivate it at a constant temperature at 37°C for 12h, and the rotation speed is 180rpm; the composition of the liquid medium is as follows: In terms of weight and volume percentage, the unit is g / 100ml, in which peptone is 1.0%, yeast powder is 0.5%, NaCl is 1.0%, and the pH value is 6.5.

[0035] Transfer the seed liquid into the sterilized liquid fermentation culture, the inocul...

Embodiment 2

[0039] The Plactet-Burman experiment optimization of embodiment 2 prodigiosin

[0040] According to experience and literature, 9 of the more important factors are selected for Plactet-Burman experiment. The factors and levels in the PLACKETT-BURMAN experiment are shown in Table 2, and the specific experimental design of the Plackett-Burman experiment is shown in Table 3.

[0041] Table 2 Factors and levels involved in the PLACKETT-BURMAN experiment

[0042]

[0043] Table 3 Design of PLACKETT-BURMAN experiment

[0044]

[0045] The seed solution was prepared according to Example 1, and the seed medium was inoculated into the above-mentioned fermentation medium according to the inoculum size of 5%, and incubated at a constant temperature of 28° C. for 48 hours with a rotation speed of 180 rpm. After fermentation, according to embodiment 1, the assay method of red pigment prodigiosin, detect the content of prodigiosin, the experimental data is calculated by Design-Expert...

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Abstract

The invention provides a bacterial strain for producing prodigiosin, belonging to Serratia marcescens, wherein the preservation number of the bacterial strain is CGMCC No.4074. The bacterial strain provided by the invention has the advantages that the haematochrome can be efficiently synthesized within a liquid fermentation culture medium by using the bacterial strain and the production efficiency is high. The bacterial strain provided by the invention has low requirements to the carbon source and nitrogen source, the carbon source can be a single carbon source or a mixed carbon source and the nitrogen source can be a single nitrogen source or a mixed nitrogen source. The bacterial strain provided by the invention is continuously reproduced for more than ten generations, the genetic stability of the bacterial strain is good and the operation is simple.

Description

technical field [0001] The present invention relates to a kind of Serratia and its application, in particular to a Serratia strain producing prodigiosin and a method thereof. Background technique [0002] Prodigiosins have the structure shown below: [0003] [0004] It is a class of secondary metabolites with important biological activities produced by a variety of microorganisms. It usually contains a methoxypyrrole skeleton structure composed of three pyrrole rings. It has anti-bacterial, anti-malarial, anti-fungal, anti-protozoal and autoimmune suppression activities such as inhibiting delayed-type hypersensitivity and host rejection after organ transplantation. In recent years, it has been found that prodigiosin can quickly kill most of the plankton that cause red tides at extremely low concentrations (one part per billion), showing great power in the control of water pollution. Studies have also found that prodigiosin has resistance to various human cancer cells ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P17/16C12R1/43
Inventor 刘晓侠陈晓丽胡星夏
Owner JIAXING UNIV
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