Dioscoreae Oppositae 'Qi' tissue culture seedling raising method

Abstract

The invention provides a Dioscoreae Oppositae 'Qi' tissue culture seedling raising method, which comprises the steps of explant selection and disinfection, callus tissue induction, adventitious bud differentiation, rooting culture, seedling training, transplanting and the like. By taking the nodular stem segments of the potted Dioscoreae Oppositae 'Qi' as explants, after the explants are disinfected by alcohol with volume concentration of 70 percent and sodium hypochlorite with weigh percentage of 2 percent, the callus tissue induction, the adventitious bud differentiation and the rooting culture are conducted on MS+NAA+BA culture mediums, and rooted seedlings are transplanted in mixed mediums for culture, wherein the mixed mediums comprise vegetable garden soil, mountain sand and peat, with the mass ratio of 4:1:1. Compared with traditional Dioscoreae Oppositae 'Qi' seedling raising methods such as a direct root tuber planting method, a cutting breeding method and the like, the Dioscoreae Oppositae 'Qi' tissue culture seedling raising method has the advantages that the genetic stability of the seedlings is good, the breeding cycle is short, the breeding coefficient is high, the operation is simple and the factory production of the seedlings can be realized. By adopting the method, not only can the problems that the quantity of the used seminal roots for the production of the Dioscoreae Oppositae 'Qi' is large, the cost is high and the characters of strain is degenerated be solved, but also technical supports can be provided for the high quality and high yield and the industrialization of the Dioscoreae Oppositae 'Qi' through large-scale seedling breeding.

Description

technical field [0001] The invention relates to a method for cultivating seedlings of Qishan medicinal group, which realizes the large-scale production of Qishan medicinal seedlings through plant tissue culture technology. It belongs to the fields of applied biology and agricultural biotechnology. technical background [0002] Yam is a common name for the root tuber of Dioscorea spp. in the family Dioscorea. It is a traditional medicinal and edible economic crop with a planting history of more than 2,000 years in my country. There are two main types of yams cultivated in my country: yams and field potatoes. The roots of Chinese yam are small and not widely distributed. They are often used for medicinal purposes except for vegetable consumption. Field potatoes are widely distributed, and most varieties belong to this species. They are mainly used for vegetable consumption. Qi yam (Dioscoreae Oppositae "Qi") belongs to the family yam species. It is a local yam planted on a l...

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Problems solved by technology

2) It is not conducive to the maintenance of caste

3) Not conducive to intensive production

Due to individual differences in seed potatoes, the time of germination or emergence of seed potatoes is often inconsistent, and the time of maturity is also different, which brings difficulties to the production management and harvesting of Qiyam.

In order to solve the above-mentioned problems, people have tried to propagate the cuttings of Qiyam single-stem node and leaf to carry out the cultivation of improved seedlings, but due to the difficulties in rooting, low survival rate, and the inability to rapidly propagate neat and consistent seedlings on a large scale, it cannot be used in actual production. be applied in

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