37 results about "Swine virus" patented technology

The invention belongs to the technical field of sample treatment equipment. The grinding effect of a traditional porcine virus sample grinding treatment device is poor. Aiming at the problems in the prior art, the invention discloses a grinding treatment device of a porcine virus sample; the grinding treatment device comprises a containing groove, a plurality of arc-shaped rods, a round block, a rotary shaft, a spindle block, a tooth ring, a plurality of grinding wheel gears and an arc-shaped friction block; an annular sliding rail is arranged at the top of the containing groove and filteringholes are formed in the bottom of the containing groove; the upper end of each arc-shaped rod is connected to the round block and the lower end of each arc-shaped rod is connected to a sliding block on the annular sliding rail; the upper end of the rotary shaft is connected to the round block and the lower end of the rotary shaft is connected to a motor; the tooth ring is connected to the rotary shaft; each grinding wheel gear is engaged with the tooth ring; the spindle block comprises a large cone frustum; an arc-shaped surface of the large cone frustum is hinged with a plurality of grindingwheels which are uniformly distributed along the peripheral direction of the arc-shaped surface; each grinding wheel is driven through the corresponding grinding wheel gear to rotate; surfaces of theadjacent grinding wheels are fitted mutually; and the arc-shaped friction block is driven through the arc-shaped rods to rotate and an inner side face of the arc-shaped friction block is fitted to thegrinding wheels. The grinding treatment device of the porcine virus sample disclosed by the invention has a good grinding effect.

The invention discloses an application of N-acetyl-L-cysteine in preparing a drug for treating or preventing porcine virus infection. The application comprises the steps: A, preparing N-acetyl-L-cysteine, absolute ethyl alcohol, tween-80, lactose, soluble starch and bran into a granule, oral liquid or powder, B, adding the granule, the oral liquid or the powder into feed or drinking water, or C, independently administering N-acetyl-L-cysteine, D, continuously using N-acetyl-L-cysteine during a whole feeding period as preventive addition and long-term usage in the feed, E, feeding the powder and the granule directly for treatment, or F, mixing the powder and the granule with daily ration, adding into the feed in the morning, at noon and in the evening every day, and continuously using the powder and the granule. N-acetyl-L-cysteine has high safety, no toxic or side effects, and antiviral activity, and can effectively prevent and treat the porcine circovirus type 2 and porcine epidemic diarrhea virus infection, reduce a mortality rate of infected pigs, and effectively increase a survival rate and productivity of the pigs.

The invention discloses a newly separated pig Getah virus HNJZ-S1 strain. The microbial preservation number of the pig Getah virus HNJZ-S1 strain is CGMCC NO 12550; the pig Getah virus is separated from a naturally infected pig group for the first time in China, and has good immunogenicity. The invention also discloses a vaccine composition which is prepared from the pig Getah virus HNJZ-S1 strain, and a preparation method thereof. The vaccine composition contains inactivated pig Getah virus strain HNJZ-S1 strain and a pharmaceutically acceptable adjuvant; the preparation method for the vaccine composition comprises the steps of virus culturing, virus liquid inactivating, aqueous phase preparing, oil phase preparing emulsifying and the like. The vaccine composition has good immunogenicity, and can produce high immunity after immunization; the attacking protecting rate is high; after inoculation, the abortion rate and the stillbirth rate of sows are obviously reduced; the incidence of piglets is remarkably reduced; the popularizing and spreading of the pig Getah virus can be effectively prevented; the vaccine composition has a broad application prospect.

The invention discloses a double fluorescent microsphere immunological detection method for pseudorabies virus gE and gB IgG antibodies. The detection method is based on a group of carboxylated fluorescent microsphere combinations based on liquid-phase protein chip technology for detecting porcine pseudorabies virus antibodies , which includes carboxylated fluorescent microspheres respectively coupled with gE truncated protein and gB truncated protein, and the amino acid sequences of gE and gB truncated protein are respectively shown in SEQ ID NO: 2 and 4. The dual fluorescent microsphere immunological detection method for simultaneous detection of PRV gE and gB IgG antibodies constructed by the invention has good repeatability, high sensitivity and good specificity, and has no cross-reaction with other common porcine viral disease positive sera. The establishment of this method can be used for the rapid differential diagnosis of wild pseudorabies virus-infected pigs and vaccine-immunized pigs and the detection of protective antibodies. It provides an important method for the monitoring of swine viral diseases and has great application value. Widespread promotion.

The invention discloses a mixed freeze-dried powder for resisting swine viral diseases and a preparation method thereof. The mixed freeze-dried powder comprises the following components in parts by weight: 90-95 parts of anti-swine virus disease yolk antibody, anti-swine virus disease 1-2 parts of transfer factor, 1-2 parts of α-interferon, 1-2 parts of antioxidant, 0.01-0.02 part of preservative, 0.2-0.3 part of inactivator, 0.05-0.1 part of selenium, 1-2 parts of polyvinylpyrrolidone 0.5-1 part of glycine, 0.5-1 part of active lactobacillus. The anti-porcine viral disease mixed freeze-dried powder of the present invention is compounded with anti-porcine viral disease egg yolk antibody, anti-porcine viral disease transfer factor and immune enhancer, supplemented with various auxiliary materials, and each component cooperates and coordinates It can significantly improve the effect of preventing and treating porcine viral diseases, and has the advantages of strong pertinence, long storage time at room temperature, high antibody titer, and good biological activity.

The invention discloses a combination of primers, a combination of probes and their application in detecting porcine viruses, a detection reagent, a kit and a detection method; the invention provides a combination of primers, a combination of probes and their application in detecting porcine viruses Application, detection reagents, kits and detection methods, establish a joint detection method for five kinds of porcine viruses, which can quickly detect five major swine diseases of ASFV, FMDV, CSFV, HP‑PRRSV and PRV wild virus, and can achieve high detection of five kinds of viruses Throughput screening improves detection efficiency and reduces reagent consumption, thereby saving costs, shortening detection time, and reducing workload; providing detection reagents that can realize joint detection of five porcine viruses, using optimized primer combinations and probe combinations, so that reagents and The kit using this reagent can specifically and sensitively detect five porcine viruses of ASFV, FMDV, CSFV, HP-PRRSV and PRV wild virus; it can simply and quickly detect five porcine virus infections with different types of nucleic acids in parallel in a single reaction tube, Realized single tube PCR to detect DNA virus and RNA virus at the same time.