Preparation method for lyophilized agent of swine interferon

A technology of porcine interferon and freeze-dried agent is applied in the field of preparation of porcine interferon freeze-dried agent to achieve the effects of improving stability, prolonging half-life and improving survival rate

Inactive Publication Date: 2016-03-09
宋宏婷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no effective and practical drug for the treatment of porcine viral diseases, so the development of highly effective antiviral drugs is an important means for...

Method used

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  • Preparation method for lyophilized agent of swine interferon
  • Preparation method for lyophilized agent of swine interferon
  • Preparation method for lyophilized agent of swine interferon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method of porcine interferon freeze-dried agent, comprising the following steps,

[0040] Step 1: Aseptically collect blood from healthy pigs, centrifuge at 4°C for 20 minutes, absorb middle layer leukocytes, and use 0.85% NH 4 After washing twice with Cl, centrifuge to collect the precipitated white blood cells and remove NH 4 Cl, according to the white blood cells: culture medium volume ratio of 1:15, add the Eagle`sMEM mixed culture medium containing 5% porcine serum to suspend, and dilute the number of porcine leukocytes at 10×10 7 Individual / ml, the porcine leukocyte suspension was prepared;

[0041] Step 2: Take a portion of porcine leukocyte suspension, add porcine leukocyte interferon containing 100 active units, and place it in a 37°C water bath shaker for 36 hours;

[0042] Step 3: Add a certain amount of Newcastle disease virus NDVII to the cell suspension according to the amount of 50 HANDVII added per milliliter of porcine leukocyte suspen...

Embodiment 2

[0047] A preparation method of porcine interferon freeze-dried agent, comprising the following steps,

[0048] Step 1: Aseptically collect blood from healthy pigs, centrifuge at 4°C for 20 minutes, absorb middle layer leukocytes, and use 0.85% NH 4 After washing twice with Cl, centrifuge to collect the precipitated white blood cells and remove NH 4 Cl, according to the white blood cells: culture medium volume ratio 1:15, add Eagle'sMEM mixed culture medium containing 5% porcine serum to suspend, and dilute the number of porcine leukocytes at 25×10 7 Individual / ml, the porcine leukocyte suspension was prepared;

[0049] Step 2: Take a portion of porcine leukocyte suspension, add porcine leukocyte interferon containing 100 active units, and place it in a 37°C water bath shaker for 36 hours;

[0050] Step 3: Add a certain amount of Newcastle disease virus NDVII to the cell suspension according to the amount of 50 HANDVII added per milliliter of porcine leukocyte suspension, a...

Embodiment 3

[0055] A preparation method of porcine interferon freeze-dried agent, comprising the following steps,

[0056] Step 1: Aseptically collect blood from healthy pigs, centrifuge at 4°C for 20 minutes, absorb middle layer leukocytes, and use 0.85% NH 4 After washing twice with Cl, centrifuge to collect the precipitated white blood cells and remove NH 4 Cl, according to leukocytes: culture medium volume ratio 1:15, add Eagle`sMEM mixed culture medium containing 5% porcine serum to suspend, and dilute the number of porcine leukocytes at 20×10 7 Between individual / ml, make porcine leukocyte suspension;

[0057] Step 2: Take a portion of porcine leukocyte suspension, add porcine leukocyte interferon containing 100 active units, and place it in a 37°C water bath shaker for 36 hours;

[0058] Step 3: Add a certain amount of Newcastle disease virus NDVII to the cell suspension according to the amount of 50 HANDVII added per milliliter of porcine leukocyte suspension, adjust the pH to...

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Abstract

The invention discloses a preparation method for a lyophilized agent of swine interferon, belonging to the field of animal vaccines. The preparation method for the lyophilized agent of swine interferon comprises the following six steps: acquiring fresh blood of a healthy pig under aseptic conditions, centrifuging the blood at 4 DEG C for 20 min, sucking up leucocytes at a middle layer, washing the leucocytes with NH4Cl with a mass fraction of 0.85% twice, then carrying out centrifugation, collecting precipitated leucocytes, removing NH4Cl, adding the leucocytes into an Eagle's MEM mixed nutrient solution containing 5% of swine serum according to a volume ratio of the leucocytes to the nutrient solution of 1: 15, carrying out suspension and diluting the swine leucocytes until the number of the swine leucocytes is in a range of 10 * 10<7>/ml to 25 * 10<7>/ml so as to prepare a swine leucocyte suspension liquid; etc. The preparation method is capable of preparing the long-acting swine virus interferon preparation which is injected once every 10 days and has good effect on swine viral diseases.

Description

technical field [0001] The invention relates to a preparation method of a freeze-dried agent, in particular to a preparation method of a porcine interferon freeze-dried agent. Background technique [0002] Porcine interferon is one of the earliest animal interferons studied, and porcine interferon was reported in the 1980s. The porcine interferons that have been discovered include INF-α, β, ω, δ and INF-γ, and now the genes have been cloned, sequenced and mapped. In recent years, the induction conditions and physical and chemical activities of porcine interferon have been further studied, and a large number of clinical trials have been carried out on porcine leukocyte interferon. Studies have shown that it has a significant effect on many viral infectious diseases of pigs, such as epidemic diarrhea, swine fever, infectious gastroenteritis, etc., as well as bovine viral diarrhea, gosling plague, lamb diarrhea, etc. Tests have confirmed that pig interference There is cross a...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K47/48A61K38/21A61K47/20A61K47/26A61K47/42A61P31/12A61K47/60
CPCA61K9/0019A61K9/19A61K38/21A61K47/20A61K47/26A61K47/42
Inventor 宋宏婷
Owner 宋宏婷
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