5results about How to "Reduce peroxidative damage" patented technology

The invention provides application of an expression inhibitor of FOXO3a in preparation of a medicine for treating acute brain injury after subarachnoid hemorrhage. The research finds that FOXO3a is highly expressed in acute brain injury after subarachnoid hemorrhage, and further finds that knock-down of FOXO3a improves SAH mouse neurological function impairment and encephaledema and reduces lipid peroxidation injury. The results show that FOXO3a is a new target spot for treating acute brain injury after subarachnoid hemorrhage. It is indicated that the FOXO3a knockout reagent or / and the FOXO3a inhibitor is a potential novel medicine for treating acute brain injury after subarachnoid hemorrhage.

The application discloses a kind of knock-out genes and its application in tomato salt stress tolerance, and the base sequence of the tomato gene is as shown in SEQ ID NO.1;The amino acid sequence of tomato SlERF.J2 protein encoded by SlERF.J2 gene is as shown in SEQ ID NO.2.The application obtains SlERF.J2 knock-out mutant strain by CRISPR / Cas9 technology, and it is found by combining salt stress experiment that the chlorophyll content of knockout plant is reduced at 300mM NaCl treatment, and the content of malondialdehyde (MDA) is reduced, indicating that the damage of lipid peroxidation is reduced.In addition, the expression of SlERF.J2 gene is inhibited under salt stress, suggesting that it may regulate tomato salt tolerance by regulating chloroplast function and antioxidant pathway.The application provides a theoretical basis for analyzing the molecular mechanism of ERF family genes and the breeding of tomato salt-tolerant varieties.

The invention discloses a biological bacteriostatic agent, a preparation method and application in prevention and control of postharvest diseases of fruits and vegetables, the bacteriostatic agent is bacillus coagulans metabolite VOCs, the preservation number of the bacillus coagulans is CGMCC NO.9951, bacteriostatic substances in the metabolite VOCs include but are not limited to benzaldehyde, acetic acid, isobutyric acid and isovaleric acid, and the bacteriostatic substances in the metabolite VOCs include benzaldehyde, acetic acid, isobutyric acid and isovaleric acid. The preparation method of the biological bacteriostatic agent comprises the following steps: diluting activated bacillus coagulans, coating the surface of an LB culture medium with the diluted bacillus coagulans, and culturing at 37 DEG C for 48 hours. The application method of the biological bacteriostatic agent in germ control in fruit and vegetable storage comprises the following steps: putting sweet potatoes and a bacillus coagulans agent in a packaging carton together, and storing in a 28 DEG C environment. The bacillus coagulans CGMCC 9951 is a generally considered safe (GRAS) strain, metabolites are safe and non-toxic, and the bacillus coagulans CGMCC 9951 is environment-friendly, convenient to produce and capable of being applied on a large scale. Chemical agents can be partially replaced for application, pesticide residues are reduced, and the storage quality of sweet potatoes is improved.

This invention relates to a salt tolerance-related protein, LcCIPK25, and its encoding gene in hybrid tulip tree (Liriodendron tulipifera), and its applications, belonging to the field of plant genetic engineering technology. The amino acid sequence of this protein is shown in SEQ ID NO.2, and the nucleotide sequence of the gene encoding this protein is shown in SEQ ID NO.1. This invention also provides a recombinant expression vector containing the aforementioned nucleic acid molecule, a recombinant host cell, and its application in improving plant salt tolerance. Overexpression of the aforementioned nucleic acid molecule in hybrid tulip tree can maintain CAT activity, alleviate leaf yellowing and oxidative damage under high salt stress, significantly reduce MDA content, and effectively enhance the plant's salt tolerance.