3results about How to "Reduce peroxidative damage" patented technology

The application discloses a kind of knock-out genes and its application in tomato salt stress tolerance, and the base sequence of the tomato gene is as shown in SEQ ID NO.1;The amino acid sequence of tomato SlERF.J2 protein encoded by SlERF.J2 gene is as shown in SEQ ID NO.2.The application obtains SlERF.J2 knock-out mutant strain by CRISPR / Cas9 technology, and it is found by combining salt stress experiment that the chlorophyll content of knockout plant is reduced at 300mM NaCl treatment, and the content of malondialdehyde (MDA) is reduced, indicating that the damage of lipid peroxidation is reduced.In addition, the expression of SlERF.J2 gene is inhibited under salt stress, suggesting that it may regulate tomato salt tolerance by regulating chloroplast function and antioxidant pathway.The application provides a theoretical basis for analyzing the molecular mechanism of ERF family genes and the breeding of tomato salt-tolerant varieties.

This invention relates to a salt tolerance-related protein, LcCIPK25, and its encoding gene in hybrid tulip tree (Liriodendron tulipifera), and its applications, belonging to the field of plant genetic engineering technology. The amino acid sequence of this protein is shown in SEQ ID NO.2, and the nucleotide sequence of the gene encoding this protein is shown in SEQ ID NO.1. This invention also provides a recombinant expression vector containing the aforementioned nucleic acid molecule, a recombinant host cell, and its application in improving plant salt tolerance. Overexpression of the aforementioned nucleic acid molecule in hybrid tulip tree can maintain CAT activity, alleviate leaf yellowing and oxidative damage under high salt stress, significantly reduce MDA content, and effectively enhance the plant's salt tolerance.