Method for carrying out taking off poison and quick breeding by using strawberry anther

Abstract

A detoxicating fast reproduction method of strawberry by anther culture includes such steps as taking the flower bud of strong strawberry, storing at 4 deg.C in refrigerator for 72 hr, disinfecting with the mixture of alcohol, corrosive sublimate and tween, sucking surface water by aseptic suction paper, taking anther, and sequentially culturing in colli inducing culture medium, regrowing culture medium, secondary culture medium, and rooting culture medium.

Description

1. Technical field

[0001] The "method for detoxification and rapid propagation by culturing strawberry anthers" of the present invention belongs to the technical field of rapid propagation of crops, and is specially used for the detoxification and rapid propagation of strawberries. 2. Background technology

[0002] Strawberry (Fragaria ananassa.Duch) is a perennial herbaceous plant of the genus Fragaria (Fragaria) of the Rosaceae (Rosaceae), 2n=8x=56, and belongs to polyploid plants. Strawberry fruit is a small berry, bright and bright, soft and juicy, moderately sweet and sour, and rich in aroma. It is one of the seven largest fruits in the world. Because cultivated strawberries have the characteristics of long fruiting period, small plants, fast reproduction, and high economic benefits, especially when they are cultivated in greenhouses, they can add varieties of varieties to the fruit market in winter and spring, especially in major festivals such as New Year’s Day and Sp...

Examples

Embodiment Construction

[0025] (1) Determination of the optimal processing parameters

[0026] 1 Determine the pre-treatment of the best flower buds and the determination of the disinfection method

[0027] 1.1 Materials and methods for best method determination

[0028] Taken from healthy strawberry flower buds in the field, the developmental stage is the uninucleate marginal stage (about 4-6mm in size), and they are cold-treated in a refrigerator at 4°C for 0h, 48h, 72h, and 96h. Take it out after the cold treatment, and carry out disinfection treatment on the ultra-clean bench.

[0029] Disinfection time and steps are as follows: flower buds are rinsed once with sterile water, soaked in 70% alcohol for 5 and 10 seconds, then rinsed once with sterile water, soaked in 0.1% mercury liter (plus Tween) for 5, 8 and 12 minutes, and finally rinsed with sterile water. Rinse with water 8-10 times (10min), specific treatment combination (Table 1). After disinfection, blot the water on the surface of the ...

1. Technical field

[0001] The "method for detoxification and rapid propagation by culturing strawberry anthers" of the present invention belongs to the technical field of rapid propagation of crops, and is specially used for the detoxification and rapid propagation of strawberries. 2. Background technology

[0002] Strawberry (Fragaria ananassa.Duch) is a perennial herbaceous plant of the genus Fragaria (Fragaria) of the Rosaceae (Rosaceae), 2n=8x=56, and belongs to polyploid plants. Strawberry fruit is a small berry, bright and bright, soft and juicy, moderately sweet and sour, and rich in aroma. It is one of the seven largest fruits in the world. Because cultivated strawberries have the characteristics of long fruiting period, small plants, fast reproduction, and high economic benefits, especially when they are cultivated in greenhouses, they can add varieties of varieties to the fruit market in winter and spring, especially in major festivals such as New Year’s Day and Sp...

Examples

Embodiment Construction

[0025] (1) Determination of the optimal processing parameters

[0026] 1 Determine the pre-treatment of the best flower buds and the determination of the disinfection method

[0027] 1.1 Materials and methods for best method determination

[0028] Taken from healthy strawberry flower buds in the field, the developmental stage is the uninucleate marginal stage (about 4-6mm in size), and they are cold-treated in a refrigerator at 4°C for 0h, 48h, 72h, and 96h. Take it out after the cold treatment, and carry out disinfection treatment on the ultra-clean bench.

[0029] Disinfection time and steps are as follows: flower buds are rinsed once with sterile water, soaked in 70% alcohol for 5 and 10 seconds, then rinsed once with sterile water, soaked in 0.1% mercury liter (plus Tween) for 5, 8 and 12 minutes, and finally rinsed with sterile water. Rinse with water 8-10 times (10min), specific treatment combination (Table 1). After disinfection, blot the water on the surface of the ...

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