Method for fusing protoplast of volvaria volvacea and pleurotus eryngii

A technology of protoplast fusion and protoplast, which is applied in the field of high-yield and low-temperature resistant Strain mushroom strains, can solve problems such as poor results, and achieve high yield, strong technical repeatability, and good genetic stability

Inactive Publication Date: 2011-03-16
SOUTH CHINA AGRI UNIV
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results obtained by t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for fusing protoplast of volvaria volvacea and pleurotus eryngii
  • Method for fusing protoplast of volvaria volvacea and pleurotus eryngii
  • Method for fusing protoplast of volvaria volvacea and pleurotus eryngii

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. The experimental methods used in the following examples, unless otherwise specified, are existing conventional methods in this technical field; the materials, reagents, etc. used, unless otherwise specified, are commercially available reagents and materials. V138 is the main variety in Guangzhou, provided by the Agricultural Science Experiment Center of Baiyun District, Guangzhou. Pleurotus eryngii Pe811 was purchased from Sanming Edible Fungi Research Institute. The relevant information of V138 and Pe811 can be found in the insert of Edible Fungi Magazine. Example 1

[0039] (1) Inoculate the V138 strain on the PDSA plate according to the conventional method, and cultivate it in a constant temperature incubator at 32°C for 3 days as the straw mushroom strain; inoculate the Pleurotus eryngii strain on the PDSA plate according to the con...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to view more

Abstract

The invention provides a method for fusing protoplast of volvaria volvacea and pleurotus eryngii. The mycelium of the volvaria volvacea and pleurotus eryngii is in enzymolysis to prepare a protoplast; the thermal inactivation of the pleurotus eryngii protoplast serves as a mark, and a PEG (polyethylene glycol)-mediated chemical method is adopted to fuse the protoplast of volvaria volvacea and pleurotus eryngii; a fusant is screened at the temperature of 0 DEG C, and a fruiting experiment is carried out to obtain a fusion strain. The method provided by the invention can effectively fuse the protoplast of volvaria volvacea and pleurotus eryngii and obtain low temperature resistant new volvaria volvacea strain with the characteristics of pleurotus eryngii. The invention provides an effectivemethod for distant fusion creation between two edible fungusiaceae with obvious complementary character.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding of edible fungi, and in particular relates to a method for obtaining a high-yield and low-temperature-resistant straw mushroom strain with the characteristics of Pleurotus eryngii by fusion of straw mushroom and pleurotus eryngii protoplasts. Background technique [0002] Straw mushroom is a kind of edible fungus, which is rich in nutrition and delicious, and is deeply loved by consumers. Straw mushroom is a typical tropical high-temperature edible fungus, which belongs to Agaricaceae, Phyllosporaceae, and the genus Agaricus in classification. Straw mushroom hyphae have poor resistance to low temperature, and are very sensitive to changes in environmental conditions, especially temperature changes: when the average temperature is below 27°C, it is difficult for straw mushroom fruiting bodies to form. Changes in temperature, especially a sudden drop in temperature, are extremely unfavorable t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/04
Inventor 郭丽琼游丽容林俊芳曾志忠
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap