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Tissue culture method of dioscorea opposita stem with axillary buds

A technology of tissue culture and axillary buds, applied in the biological field, can solve the problems of unfavorable variety stability, etc., and achieve the effects of fast reproduction speed, high reproduction coefficient and good genetic stability

Inactive Publication Date: 2012-09-12
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research of yam tissue culture has been reported both at home and abroad, but most of them focus on the induction of callus such as stem segments and zero surplus seeds, which is not conducive to maintaining the stability of varieties.

Method used

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  • Tissue culture method of dioscorea opposita stem with axillary buds
  • Tissue culture method of dioscorea opposita stem with axillary buds
  • Tissue culture method of dioscorea opposita stem with axillary buds

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Embodiment Construction

[0025] The present invention will be further illustrated by the example of obtaining Chinese yam tissue cultured seedlings based on explants induced by explants with axillary buds.

[0026] 1 Material Handling

[0027] The tubers of Tiebang yam from Wen County, Henan Province were buried shallowly in fine sand, cultivated in a constant temperature (25°C) cultivation room, and used when the seedlings grew.

[0028] 2 Medium sterilization and culture conditions

[0029] Before autoclaving, adjust the pH value of the medium to 5.8, and keep it at 121°C for 20 minutes for sterilization. The temperature of the culture room is 25±2°C, the light intensity is 1200-1500 lx, and the light is 12 hours a day. The mass fraction of agar in the medium is 7g / L, and the mass fraction of sucrose is 30g / L.

[0030] 3 Sterilization test of explants

[0031] 3.1 Experimental design

[0032] Take the tender stems of the iron stick yam, remove the leaves, cut into 2-3cm stem segments with axill...

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Abstract

The invention discloses a tissue culture method of a dioscorea opposita stem with axillary buds. A dioscorea opposita stem with axillary buds is selected as an explant, and through a series of operations of bud induction, mulitiplication and rooting culture, a dioscorea opposita test-tube plantlet is finally formed. The method includes medium components for a plurality of stages of cultivation and a processing method, and a complete tissue culture seedling can be acquired within about twenty days. Through the method, dioscorea opposita tissue culture seedling can be acquired through the induction of the explant of the dioscorea opposita stem with axillary buds, so indoor mass propagation of the test-tube plantlets is feasible, and breeding and industrial production of dioscorea opposita tuber can be realized. The tissue culture method has the characteristics of high propagation coefficient, fast propagation speed, high propagation efficiency and good genetic stability and is free of the influence of environment conditions, and the seedlings have fewer virus.

Description

technical field [0001] The invention "a method for tissue culture of the stem section of yam with axillary buds" is to obtain test-tube seedlings through tissue culture from the explants of the stem section of yam with axillary buds, and belongs to the field of biotechnology. Background technique [0002] Chinese yam (Dioscorea Opposita Thunb.) is a perennial herbaceous twining vine of the genus Dioscorea in the family Dioscorea. It is planted in all parts of my country. Because of its high nutritional value and medicinal value, it is an authentic medicinal material and traditional export commodity in my country, and its products sell well in Southeast Asia, Japan and other countries. Due to the long-term vegetative propagation, not only the amount of seeds used is large and the propagation speed is slow, but also the continuous accumulation of viruses leads to the degradation of the species, which makes the planting area decrease year by year, seriously affecting the improv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 韩晓勇殷剑美张培通彭琦郭文琦张恒友
Owner JIANGSU ACAD OF AGRI SCI
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