Method for producing momordica grosvenori group seedling with single culture medium

Abstract

A method for producing a grosvenor momordica tissue culture seedling by a single culture medium uses an MS solid medium during the producing process and is characterized in that the angustimycin of 0.1 to 0.5mg L<1> is added into a normal MS solid medium to produce the grosvenor momordica tissue culture seedling. As the medium singly prepared by the angustimycin can be simultaneously used on each technical tache of 'axillary bud explant starting', 'proliferation and expanding propagation' and 'proliferated seedling rooting', thereby greatly simplifying the producing operation procedures of the grosvenor momordica tissue culture seedling. In particular, the angustimycin can effectively control the generation of a callus during the culturing process of the grosvenor momordica tissue culture seedling in particular, which is beneficial to the genetic stability; thereby improving the quality of the tissue culture seedling.

Description

technical field [0001] The invention belongs to a method for producing tissue cultured seedlings of Luo Han Guo with a single culture medium. technical background [0002] Luo Han Guo Siraitia grosvenori (Swingle) C. Jeffrey, also known as Han Guo, Lahan Guo, Longevity Fruit, Fairy Fruit, Luo Huangzi, False Bitter Melon, Light Fruit Wood Softshell Turtle, etc., is a perennial herbaceous vine of the genus Siraitia Merr of Cucurbitaceae Cucurbitaceae, male and female Different plants, native to my country, are also one of the most important traditional Chinese medicinal materials in my country and Guangxi. They have high application value and broad development prospects in medical and health care. In the north of Guangxi, Mangosteen is mostly used as a pillar industry for development. Some cities and counties in Guangdong, Jiangxi, Hunan, Guizhou and other provinces are also actively developing monk fruit cultivation. Because tissue culture seedlings can be planted on flat gr...

AI Technical Summary

Problems solved by technology

What's more troublesome is that the biological effect of 6-BA in Luo Han Guo tissue culture is significantly different from that in other plant tissue culture. As long as it is within the effective concentration, it will inevitably induce the occurrence of a large number of callus, and Appears in the whole process of tissue culture seedling production

Considering genetics, the occurrence of a large number of calluses will affect the genetic stability of tissue cultured seedlings, eventually leading to various poor-quality variations and adverse events in field cultivation (such as low resistance, failure to bloom and bear fruit, etc.) Producers and growers of seedlings bring great potential risks and losses