Obtaining precocial mutant of sugar beet by ion implantation, and verification technique

A technology of ion implantation and mutants, which is applied in the fields of mutant preparation, treatment of microorganisms with electricity/wave energy, and plant gene improvement, etc. It can solve the problems of long existence period, ineffective effect, and inability to obtain innovative sugar beet materials, etc.

Inactive Publication Date: 2007-12-26
ECONOMIC CROPS RES INST XINJIANG ACAD OF AGRI SCI
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Problems solved by technology

[0006] At present, due to many factors, the sugar content and yield of sugar beet have decreased, and the disease is serious. Therefore, it is urgent to solve the problems of increasing sugar beet yield, sugar content and disease resistance, and solving the rational distribution of early-maturing varieties. However, due to the lack of germplasm resources for beet breeding
Sugar beet breeding usually produces new gene sources through hybridization, and the existence cycle is too long. In the absence of gene sources, new sugar beet innovative materials cannot be obtained, which restricts the development of sugar beet breeding
In the field of physical and chemical mutagenesis of sugar beet, mutagenesis methods such as spaceflight and cobalt 60 have been applied successively, but the effects are not obvious

Method used

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  • Obtaining precocial mutant of sugar beet by ion implantation, and verification technique

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: Mutagenesis test one in ion implantation in sugar beet breeding

[0040] Select the sugar beet excellent parent 7201, 7201-1 that the inventor has for use and dry seeds of many grains, with the N that energy is 35Kev + Ion-to-beet seed implantation with a dose range of 2×10 16 N + / cm 2 -7×10 16 N + / cm 2 . The number of pulses ranged from 40 to 60 times, and the shells were broken and the shells were not broken, and single and multi-seed controls were set respectively. The treated seeds were put into gauze bags. Seeds were soaked in warm water at 20°C for 18 hours, and artificially sowed on demand. Morphological changes were observed and recorded during the growth period. Yield and sugar were measured at harvest. And carry out isoenzyme detection.

[0041] 1 Materials and methods

[0042] 1.1 The test materials are treated in 3 rows, the row length is 6 meters, the spacing between plants and rows is 50×20cm, and the ratio between rows is arran...

Embodiment 2

[0050] Embodiment 2: Mutagenesis test two in ion implantation in sugar beet breeding

[0051] Materials and Methods

[0052] 1. The test materials are dry seeds of 7201 and 7201-1, which are excellent parent materials of sugar beet. + Inject beet seeds, choose to inject 2×10 16 N + / cm 2 , 3×10 16 N + / cm 2 , 4×10 16 N + / cm 2 , 5×10 16 N + / cm 2 , 6×10 16 N + / cm 2 , 7×10 16 N + / cm 2 , 8×10 16 N + / cm 2For different doses, the number of pulses was selected as 30, 35, 40, 45, 50, 55, 60, 65, and 70 times, respectively. There are 3 rows for each treatment, the row length is 6 meters, the spacing between plants and rows is 50×20cm, and the row ratio is arranged.

[0053] 2. M 1 Generation of seeds for field observation and identification. Participate in 9 samples of materials, plot length 6 meters, 3 row plots, plant row spacing 50 × 20 cm, repeat three times, random block group.

[0054] 3. Combine 3 copies of 7201 and 7201-1 Annual M 1 The energy of ...

Embodiment 3

[0059] Embodiment 3: peroxidase, isozyme and N in the leaf + injected relationship

[0060] Peroxidase is widely present in plants and is an enzyme with high activity. It is closely related to the growth and metabolism of crops. From the analysis of the peroxidase band of sugar beet leaves, it is found that N + There are obvious differences between the injected and the control enzyme bands, see Figure 1. From the atlas of attached drawing 1, numbered by letters: B (single) 4×10 16 , 40 times, C (single) 4×10 16 , 60 times, H (multiple) 6×10 16 , 40 times, I (more) 4 × 10 16 , 60 times, L (multi-breaking) 4×10 16 , 60 treatments have 2 more bands, G (more) 4×10 16 , 40 times, M (multi-break) 6×10 16 , 60 times to process a few more bands. In the single-seed treatment, the coloring of the enzyme gradually became lighter as the number of pulses increased. In the multi-seed treatment, with the increase of the number of pulses and the injection dose, the coloring of the en...

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Abstract

This invention provides an ion implantation method for inducing early maturing vulgaris mutant, and RAPD identification method. The early maturing vulgaris mutant is obtained by performing ion implantation on seeds of homozygous vulgaris inbred line 7201. The early maturing vulgaris mutant has high stability in field. It is proven by RAPD analysis that ion implantation induces DNA level changes in vulgaris. This invention also studies the influence of optimal energy and optimal ion implantation dosage on the hybrid and the parent plants. The method can be used for developing vulgaris cultivars.

Description

field of invention [0001] The invention relates to the field of crop genetic breeding and molecular biology technology, in particular to the technical field of using ion implantation to mutagenize sugar beets to obtain early-maturing mutants and its verification. Background technique [0002] In the mid-1980s, a group of scientific and technological workers such as Yu Zengliang, a researcher at the Institute of Plasma Physics, Chinese Academy of Sciences, discovered the biological effects of ion implantation into living organisms: injecting various ions into cells, through the electrical energy mass The combined action of the cells can not only affect the physiological functions of the cells, but more importantly, it can change the genetic material of the cells; due to the effect of energy and the doping of the quality, a large number of free radicals will be formed, which can cause the genetic material in the living cells to be damaged. DNA hydrogen bond breaks, double-stra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N13/00A01H1/06
Inventor 王燕飞曾宪贤符子华刘华君沙红张立明曲延英高卫时刘军高文伟杨洪泽白晓山
Owner ECONOMIC CROPS RES INST XINJIANG ACAD OF AGRI SCI
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