The invention discloses a device for locating a CT (Computed Tomography)-guided percutaneous biopsy and seed implantation template. The device comprises a CT bed, a support, a first sliding rail, first sliding blocks, a first motor, a first screw rod, first nuts, a connecting plate, a second sliding rail, a second sliding block, a second motor, a second screw rod, a second nut, a moving plate, a third motor and a controller. The device for locating the CT-guided percutaneous biopsy and seed implantation template has the advantages of ingenious structure, powerful functions and simple operation; by using the device, the puncturing and locating accuracy can be greatly improved; moreover, the working efficiency of medical workers is improved.