Method for breeding Poria cocos

The technology of Poria cocos and seedlings is applied in the field of cultivating microorganisms, which can solve the problems of inability to meet demand and low yield, and achieve the effects of low planting cost and protection of forest resources.

Inactive Publication Date: 2006-02-15
SAAS BIOTECH & NUCLEAR TECH RES INST
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Problems solved by technology

It can be harvested from April to June in the second year. Although this method ca

Method used

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Experimental program
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Embodiment Construction

[0016] The specific process of bacterial culture is as follows:

[0017] 1. Select high-quality Ling species

[0018] The standard is good quality, no pests and diseases, large individuals, nearly spherical, firm texture, red skin, white flesh, rich juice, and strong smell of Poria cocos sclerotia.

[0019] 2. Isolation and cultivation of mother species

[0020] The separation method of the parent species is as follows: rinse the species with sterile water several times, then wipe dry with gauze, and finally scrub the skin of the species with 75% alcohol, and after scrubbing with 0.1% mercury liter, carry out ultraviolet sterilization; Under certain conditions, use a sterile knife to cut open the seedlings, use an inoculation shovel sterilized by alcohol flames to take out soybean-sized meat from the deep part of the seedlings, and insert them into the inclined surface of the test tube;

[0021] The formula of the mother seed medium is: 200 grams of potatoes, 20 grams of glu...

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Abstract

The invention discloses a method for cultivating Poria cocos, belonging to the technique field of microbiological cultivating, which comprises: choosing good Poria cocos seeds by means of bacterial breeding, preserving and culturing the parent seeds, producing and breeding the initial species and cultivating species on the base of the former step; the parent species culturing comprising separating the parent species and culturing in the culture medium. By employing the method in this invention, bacterial filament and sclerotium can be produced, the culture medium is prepared artificially in the culture of parent species, original species and cultivating species course, and does not need natural pine and thus there is no need for trees cutting. The method is suitable for mass production and the planting cost is very low.

Description

technical field [0001] The invention belongs to a method for cultivating microorganisms, in particular to a method for cultivating poria cocos. Background technique [0002] Poria cocos is a perennial saprophytic fungus belonging to the fungus phylum, which has medicinal value and is a commonly used bulk traditional Chinese medicine. Wild tuckahoe usually grows at an altitude of more than 500 meters and grows on decaying pine roots or pine sections about 20 centimeters underground. The growth and development of Poria cocos can be divided into two stages, that is, the mycelium growth stage and the sclerotia formation stage. The mycelium growth stage is mainly that the mycelium absorbs water and nutrients from the pine wood, and reproduces a large number of vegetative bacteria. For a long time, it becomes a sclerotium, and when the sclerotia is formed, it is cultivated to be called the knotting stage. At present, the methods of artificially cultivating Poria cocos mainly inc...

Claims

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Application Information

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IPC IPC(8): A01H15/00A01G1/00
Inventor 何俊蓉李萍徐利远
Owner SAAS BIOTECH & NUCLEAR TECH RES INST
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