Hypsizygus marmoreus GJ5 strain as well as SSR marker primers and application thereof

A technology for labeling primers and crab-flavored mushrooms, applied in the fields of application, botanical equipment and methods, biochemical equipment and methods, etc., can solve problems such as differences in the quality of crab-flavored mushroom strains, and achieve high accuracy, cost reduction, and detection. short time effect

Active Publication Date: 2021-01-29
东营市菇健生物科技有限公司
View PDF4 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the differences in the cultivation and growth conditions of different crab-flavored mushroom strains, and the large differences in the quality of different crab-flavored mushroom strains, the purpose of the present invention is to provide a strain of mushroom-healthy crab-flavored mushroom GJ5 strain and its SSR-labeled primer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hypsizygus marmoreus GJ5 strain as well as SSR marker primers and application thereof
  • Hypsizygus marmoreus GJ5 strain as well as SSR marker primers and application thereof
  • Hypsizygus marmoreus GJ5 strain as well as SSR marker primers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Development and application of SSR-labeled primers for Mushroom GJ5 strain

[0043] The said GJ5 strain of crab-flavored mushroom is an industrial crab-flavored mushroom strain independently developed by the laboratory. This strain has the advantages of high yield, stable strain properties, high concentration of liquid strains, strong vitality, neat fruiting, and good taste.

[0044] The Gujian white jade mushroom GJ5 strain developed by the present invention is preserved for strains. The preservation unit: China Typical Culture Collection Center; Address: Wuhan, China. Wuhan University; preservation date: September 16, 2020; Hypsizygusmarmoreus GJ5 strain The deposit number is CCTCC M 2020506.

[0045] (1) Sequence analysis and primer design of the SSR markers of the crab-flavored mushroom:

[0046] The genome of the GJ5 strain was extracted for whole genome sequencing, and the obtained genome size was 45.1M. The SSR marker sequence was searched in the geno...

Embodiment 2

[0072] Example 2: SSR-marked fingerprints of fruiting bodies in different parts of No. 5 Gujian Xieweigu No. 5

[0073] The SSR marker in the present invention is based on the 150 simple repeats (SSRs) found on the genomic DNA using the SSRhunter1.3 software after the whole genome sequencing of the crab-flavored mushroom, and specific primers are designed according to its upstream and downstream gene sequences, and the collected After PCR amplification, electrophoresis was performed to obtain polymorphic marker fragments of the crab-flavored mushroom species. SSR marking has the advantages of simple detection method, less requirement for sample volume, no requirement for sampling position, good repeatability, and short detection time. The present invention screens a large number of SSR primers, and finally obtains 5 pairs of primers with high polymorphism and good specificity. The fragment combination obtained by using these 5 pairs of primers can be used for 24 kinds of white...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a hypsizygus marmoreus GJ5 strain as well as SSR marker primers and application thereof. The preservation number of the hypsizygus marmoreus GJ5 strain is CCTCC M 2020506; theSSR marker primers comprise five pairs of SSR specific marker primer combinations, and the five pairs of SSR specific marker primers form a fingerprint spectrum. The application comprises the following detection steps: extracting genome DNA of hyphae or sporocarp of a strain to be detected; carrying out SSR molecular marker amplification; carrying out electrophoresis detection; and comparing an electrophoretic band with the fingerprint spectrum, wherein the bacterial strain consistent with the fingerprint spectrum is the hypsizygus marmoreus GJ5 strain. Compared with conventional morphologicaldetection, antagonism experiments and fruiting experiment methods, the SSR fingerprint has the advantages of being short in detection time, high in accuracy, good in repeatability and the like, and the SSR fingerprint constructed through the SSR fingerprint can be used for distinguishing the hypsizygus marmoreus GJ5 strain from 23 main cultivated varieties of hypsizygus marmoreus.

Description

technical field [0001] The invention belongs to the technical field of detection of edible fungus strains, and in particular relates to a mushroom strain GJ5 and its SSR marker primer and application. Background technique [0002] Crab-flavored mushroom (Hypsizygus marmoreus) is a gray true chrysanthemum, belonging to the order Agaricaceae, white mushroom family, and the genus Amygium. Polysaccharides, proteins and various trace elements have high economic value and market potential. [0003] Bacterial strains in industrial cultivation are the key to high and stable yields. Gujian Xieweigu No. 5 strain is independently developed by the laboratory. It has high yield, stable strain properties, high concentration of liquid strains, strong vitality, and neat fruiting Good taste and other advantages. Edible fungi have the characteristics of asexual reproduction, and strains can be obtained by simple tissue separation of fruiting bodies. Therefore, strengthening the identificat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01H15/00C12Q1/6895C12Q1/6858C12Q1/04C12N15/11C12R1/645
CPCA01H15/00C12Q1/6895C12Q1/6858C12Q2600/156C12R2001/645C12N1/145C12Q2531/113C12Q2565/125
Inventor 于浩贾毅郭立忠徐丽丽
Owner 东营市菇健生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap