Fungus for promoting aquilaria plants to generate agilawood and application of fungus

A kind of agarwood, microbial strain technology, applied in plant products, applications, fungal products and other directions, can solve the problems of plants such as white wood incense, and achieve the effect of strong environmental tolerance

Active Publication Date: 2014-03-26
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of Aquilaria (Aquilaria) plants such as Aquilaria spruceae, aiming at the existing tech

Method used

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  • Fungus for promoting aquilaria plants to generate agilawood and application of fungus
  • Fungus for promoting aquilaria plants to generate agilawood and application of fungus
  • Fungus for promoting aquilaria plants to generate agilawood and application of fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Isolation, screening and identification of Fomitopsis sp. ASAF01 strain

[0022] The invention utilizes the tissue separation method to isolate the bacterial strain. The specific method is as follows: take a natural agarwood sample from Nanyao Garden in Wanning City, Hainan Province, wash it under the tap for 30 minutes, then disinfect it with 70% alcohol for 3-5 minutes, rinse it with sterile water for 3 times, and then wash it with 10% alcohol. Soak in (mass percent concentration) sodium hypochlorite for 5 minutes, then rinse with sterile water for 3 times, then cut into 2mm-3mm small pieces and put them in the culture medium containing streptomycin, and use sterile water for the last time as a control. After 3d, check and pick strains every day and purify until 2 weeks.

[0023] Cultivate the isolated strains in PDA liquid medium for 3-15 days, inoculate fungal hyphae, or use the whole body aroma technology to input fermentation broth and sterilized filtr...

Embodiment 2

[0025] Example 2. Detection of growth conditions of Fomitopsis sp. ASAF01 strain.

[0026] Inoculate the mycelium of Fomitopsis sp. ASAF01 in the center of a petri dish containing solid PDA medium, and set it at 10°C, 15°C, 20°C, 25°C, 28°C, 30°C, 35°C , 40°C incubator, and it was found that ASAF01 can grow at 15°C to 40°C, but 35°C is the most suitable for growth. Adjust the pH value of the PDA medium with 0.1mol / L HCL and 0.1mol / L NaOH to make liquid PDA medium with pH values ​​of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 , the mycelia were inoculated into liquid PDA medium with different pH values, and cultured on a shaker at 35°C at 200 rpm. It was found that the strains could grow from PH2 to PH11, and the optimum pH value was 7.

Embodiment 3

[0027] Example 3. Verification of the effect of Fomitopsis sp. ASAF01 on promoting the production of agarwood

[0028] Cultivate the Fomitopsis sp. ASAF01 strain for 3-15 days to obtain the strain mycelia, fermentation broth, and sterilized filtrate. According to the size of the tree, inoculate in different doses, drill holes, or use the whole body aroma technology to inoculate the bacteria-containing The fermentation liquid of the body, the filtrate of the fermentation liquid to remove the bacteria body is inoculated with white wood to promote the aroma, and the specific inoculation method is as follows:

[0029] 1. Acquisition of inoculation material: The mycelium obtained by culturing Fomitopsis sp. ASAF01 strain in liquid medium is the inoculation mycelium 1. The hyphae are inoculated into the PDA liquid medium, and shake cultured at 35° C. and 200 rpm for 3-15 days to obtain a culture solution, which is bacterial solution 2. At the same time, after culturing Fomitopsissp...

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Abstract

The invention discloses a fungus for promoting aquilaria plants to generate agilawood and application of the fungus. The fungus is Fomitopsis sp. ASAF01, and the preservation number in the China General Microbiological Culture Collection Center is CGMCC NO.7802. The Fomitopsis sp.ASAF01 can promote the heartwood formation of aquilaria sinensis after the Fomitopsis sp. ASAF01 is inoculated for six months, and the middle of the formed agilawood is not easy to rot. Furthermore, the strain can still grow at the temperature of 15-40 DEG C and pH value of 2-11 to indicate that the Fomitopsis sp.ASAF01 has strong environmental tolerance.

Description

technical field [0001] The present invention relates to the field of microbes and microbe applications, in particular to a fungus that promotes the production of agarwood from Aquilaria genus plants such as Agarwood agaricus and its application. The final filtrate, the active ingredients in the filtrate and the concentrated preparation of the bacterial solution / filtrate can promote the aroma formation of the agarwood plant. Background technique [0002] Agarwood is resinous wood of Aquilaria (Thymelaeaceae) plants. Aquilaria contains 13 species. The most common ones are Aquilaria malaccensis, Aquilaria crassna and Aquilaria sinensis. ). Agarwood is the only plant resource of the precious domestic medicinal material agarwood, mainly produced in Hainan, Guangdong, Yunnan, Guangxi and other places in my country. Malay agarwood (A.malaccensis) and Vietnamese agarwood (A.crassna) are mainly produced in Indonesia, Malaysia, Vietnam, Thailand and other places. Agarwood has been ...

Claims

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Application Information

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IPC IPC(8): A01H15/00A01G7/06
Inventor 魏建和陈旭玉杨云刘洋洋张争甘炳春苏智
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
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