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Inonotus obliquus and method for extracting triterpennoids from same

A technology of Inonotus obliquus and triterpenoids, applied in the field of Inonotus obliquus and triterpenoids extracted from Inonotus obliquus, which can solve the problems of low utilization rate of active ingredients and low extraction rate

Inactive Publication Date: 2011-01-05
辽宁储阳生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And adopt wild direct use, its active ingredient utilization rate is low
Triterpenoids have high application value in medicine, but the extraction rate of triterpenoids from Inonotus obliquus is relatively low

Method used

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  • Inonotus obliquus and method for extracting triterpennoids from same
  • Inonotus obliquus and method for extracting triterpennoids from same
  • Inonotus obliquus and method for extracting triterpennoids from same

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Experimental program
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Embodiment Construction

[0037] Method for extracting triterpenes from Inonotus obliquus (Inonqqus obliquus LNUF008):

[0038] 1) Strain activation: Inonotus obliquus LNUF008 CCTCC M209280 was placed on the PDA slant medium, and cultured in a 28°C incubator for 5 to 7 days;

[0039] The composition of PDA slant medium is: 200 grams of potatoes, 20 grams of glucose, KH 2 PO 4 1 g, MgSO 4 ·7H 2 O 0.5 g, agar 2 g, deionized water 1000 ml, pH natural;

[0040] 2) Seed liquid preparation: pick the activated strains into two 500 ml Erlenmeyer flasks containing 200 ml of PDA liquid medium with an inoculation loop, and cultivate them at 28°C and 180 rpm for 10 to 12 days;

[0041] PDA liquid medium: 20 grams of glucose, 0.2 grams of peptone, VB 1 0.005g, KH 2 PO 4 1 g, MgSO 4 ·7H 2 O 0.5 grams, 1000 milliliters of deionized water, pH 6.5;

[0042] 3) Fermentation liquid preparation: the seed liquid is received into the liquid comprehensive culture medium of the fermenter with the inoculation amount ...

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Abstract

The invention relates to Inonotus obliquus and a method for extracting triterpennoids from the same. The adopted technical scheme is that the Inonqqus obliquus LNUF 008 has the collection number of CCTCC M 209280. The method for extracting the triterpennoids from the inonotus obliquus comprises the following steps of: activating the inonotus obliquus to prepare seed liquor; inoculating the seed liquor in an inoculation amount which accounts for 2 percent of the volume of fermentation liquor into a fermentation tank liquid synthetic medium, fermenting, performing suction filter on the fermentation liquor, drying at the temperature of 45 DEG C, filtering out mycelia, pouring the mycelia into a mortar, grinding the mycelia into powder, adding an organic solvent in a volume which is 10 times mass of the powder, and immersing for 24 hours; performing ultrasonication at the temperature of 50 DEG C and the frequency of 77 KHz for 60 minutes; and centrifuging for 10 minutes under the condition of 5,000 revolutions / minute, removing sediment, collecting supernatant, and concentrating under reduced pressure to obtain a target product. The triterpennoids extracted from the inonotus obliquus have the advantages of high content and high recovery rate, and provide the feasibility for large-scale production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an Inonotus obliquus and a method for extracting triterpenes from the Inonotus obliquus. [0002] The Inonotus obliquus referred to in the present invention is named: Inonotus obliquus (Inonqqus obliquus LNUF008), which was preserved in the China Center for Type Culture Collection on November 24, 2009, and the number is CCTCC M 209280. Background technique [0003] Inonotus obliquus, Inonotus obliquus, is also known as Inonotus obliquus and Inonotus obliquus in China. Belongs to Basidiomycotina, Laminomycetes, Phyllomycetes, and Pseudobacteriaceae. [0004] Morphology of Inonotus obliquus: Its fruiting bodies are tumor-like (sterile lumps), 25 to 40 cm in diameter, dark in color, deeply cracked on the surface, very hard, and sometimes brittle. Spores broadly elliptic to ovate, smooth, with bristles. The mycelium is a two-line mycelium system, with reproductive hyphae a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H15/00C12P15/00
Inventor 刘宏生朱春玉郭立雄宋雅娜艾海新郑方亮朱俊丰
Owner 辽宁储阳生物科技开发有限公司
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