Tissue culture efficient breeding and seedling growing method of hybrid orchid Huangjinxiaoshentong

A technology of tissue culture and hybrid orchid, applied in the field of plant tissue culture, to achieve the effect of saving manpower and material resources, strong resistance and high quality

Active Publication Date: 2015-04-22
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report about hybrid orchid golden

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1:

[0017] (1) Explants and their treatment

[0018] Select the young shoots of a single plant of the excellent hybrid orchid 'Golden Prodigy' that has bloomed and grown vigorously under protected cultivation as explants, and spray the plants twice with chlorothalonil and streptomycin, control the water for 7 days, and then use a sharp scalpel blade Cut the young shoots from the base, then soak them in 75% alcohol aqueous solution for 30 seconds on the ultra-clean workbench, rinse them with sterile water, soak them in 0.1% HgCl solution for about 5 minutes, and rinse them with sterile water for 2~ 3 times, and then repeat twice with 0.1% HgC1 solution. After disinfection twice, peel off the bracts of dormant buds on the sterile inoculation tray, and then soak them in 0.05% HgC1 solution for 2-3 minutes. Rinse with water for 5 to 8 times to obtain surface-sterilized shoots.

[0019] (2) induced rhizome

[0020] Cut a new incision at the base of the surface-st...

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PUM

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Abstract

The invention discloses a tissue culture efficient breeding and seedling growing method of hybrid orchid Huangjinxiaoshentong. The tender shoot of the hybrid orchid Huangjinxiaoshentong is adopted as an explant and undergoes tissue culture to induce rhizome, and the rhizome is cultured only through using a medium in order to simultaneously complete propagation of the rhizome and the differentiation and growth of sprouts. The method has the advantages of large quantity, fast seedling emergence, high breeding rate and strong stability, obtained plants have the characteristics of strong sprouts, high quality and strong resistance, and the method can realize successful development of the factory production of the rapid breeding of hybrid orchid seedlings, and provides a method for the tissue culture of other orchid-like plants.

Description

Technical field: [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture and high-efficiency propagation and seedling cultivation of a hybrid orchid 'Golden Little Prodigy'. Background technique: [0002] Cymbidium Golden Elf 'Sundust' was obtained by crossing Sijilan and Hutou Orchid in Taiwan Province of my country more than 10 years ago. Its flowering phenology and traits are closer to Jianlan's growth characteristics, so it is generally classified as Jianlan variety at present. It has the characteristics of golden and elegant flower color, mellow flower fragrance, and free and easy leaf appearance. Since it entered the market, it has been favored by consumers because of its "high quality and low price". [0003] At present, there have been papers on the tissue culture of hybrid orchids in China, which adopt the protocorm approach and cluster bud approach to regenerate seedlings. The test-tube plantlets ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 吴坤林张建霞曾宋君段俊
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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