Rapid seedless grape breeding method

Abstract

The invention discloses a rapid seedless grape breeding method in the technical field of plant cultivation, which comprises the following steps: step 1, selecting male and female parents and hybridizing: selecting seedless excellent varieties as female parents and male parents respectively, performing artificial inflorescence emasculation on the female parents by using tweezers, bagging and marking, and performing artificial pollination; step 2, embryo rescue: shearing and taking back the hybridized clusters, washing the fruits with running water for 2-4 hours, transferring the fruits into a bottle for disinfection, stripping ovules, inoculating the taken ovules into an embryo development culture medium for dark culture, and after 8-10 weeks, cutting off the embryos and inoculating the embryos into an embryo germination culture medium for light culture; and step 3, transplanting and seedling hardening. The method is simple and easy to operate, through the design of the functional scissors, a sample is disinfected and coated with a culture solution while shearing is achieved, then the sample is disinfected, the activity of the sample is kept, and a good foundation is laid for subsequent seedless grape cultivation.

Description

Technical Field

[0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a rapid breeding method for seedless grapes. Background Art

[0002] Grape (scientific name: Vitis vinifera L.) is a woody vine plant of the genus Vitis in the family Vitaceae. It is widely used for fresh eating, winemaking, and processing into foods such as raisins and grape juice due to its many cultivars, strong adaptability, and nutritious fruits. It is one of the main fruit trees cultivated in various parts of my country.

[0003] China is a major producer of grapes (Vitis vinifera L.) in the world. Since 2011, China has ranked first in the world in terms of table grape production. Since 2014, China's grape cultivation area has jumped to second place in the world. By the end of 2015, the cultivated area reached 799,000 hm2. 2 , the output reached 13.669 million tons. my country's grape production is mainly fresh grapes, accounting for about 80%, and the standardized production of grape seedlings is the basis for the development of the industry.

[0004] Seedless grapes can be used for fresh eating and dried. Due to their good taste, they have a very broad market development prospect and are the breeding direction of breeders around the world. Since seedless grapes have no seeds, conventional breeding methods cannot be used, and only young embryo rescue technology can be used. The emergence of embryo rescue technology makes it possible to use seedless grapes as female parents, shortening the breeding cycle while increasing the seedless rate of hybrid offspring, providing a new method for seedless grape breeding. Summary of the invention

[0005] The purpose of the present invention is to provide a rapid seedless grape breeding method. Through the design of functional scissors, the sample can be disinfected and smeared with culture solution while cutting, thereby achieving disinfection of the sample and maintaining the activity of the sample, laying a good foundation for subsequent seedless grape cultivation.

[0006] In order to achieve the above object, the technical scheme of the present invention is as follows: a method for rapid breeding of seedless grapes, comprising the following steps:

[0007] Step 1: Select the parents and hybridize: Select seedless varieties as the female and male parents respectively, and use tweezers to manually emasculate the inflorescence of the female parent, and mark it with a bag. When transparent mucus appears on the stigma 2-3 days after emasculation, perform artificial pollination;

[0008] Step 2, embryo rescue: cut the hybridized ears and retrieve them, and rinse the fruits with running water for 2-4 hours, transfer the fruits to bottles for disinfection, and use a peeler to peel the ovules, and inoculate the removed ovules on embryo development medium for dark culture. After 8-10 weeks, dissect the embryos and inoculate them on embryo germination medium for light culture;

[0009] Step 3, transplanting and hardening the seedlings: Take out the seedlings after embryo rescue, remove the culture medium, and put them into pots with sterilized matrix, place them in natural sunlight and temperature to harden the seedlings, and transplant them to the field for planting after 2-3 months.

[0010] Furthermore, in step 1, pollination needs to be performed continuously 2-3 times, with an interval of 24 hours between each pollination.

[0011] Further, in step 2, the fruit is rinsed and disinfected by using a rinsing device, which includes a rinsing body, an opening is provided on one side of the rinsing body, a closing plate is provided on the opening, a plurality of nozzles capable of connecting clean water and disinfectant are provided on the top of the rinsing body, a mounting plate is provided below the nozzle, the mounting plate is located on one side of the opening, sliders are provided on both sides of the mounting plate, a sliding groove corresponding to the position of the slider is provided on the inner wall of the rinsing body, and the mounting plate and the rinsing body are laterally slidably matched through the sliding groove of the slider;

[0012] The mounting plate is provided with a plurality of hanging holes, each of which is symmetrically provided with guide holes on both sides, and the bottom of each guide hole is fixedly connected with an oblique guide tube, which is inclined toward one side of the hanging hole;

[0013] A disinfection soaking frame is provided under the mounting plate, an electric control cylinder is fixedly connected to the bottom of the disinfection soaking frame, the cylinder body of the electric control cylinder is fixedly connected to the inner bottom wall of the flushing body, grooves corresponding to the position of the slider are provided on both side walls of the disinfection soaking frame, a flow port is provided on one side of the disinfection soaking frame, and a first one-way valve is provided on the flow port.

[0014] Furthermore, a filter assembly is provided on one side of the flushing body, the filter assembly includes a filter body, a partition plate is fixedly connected inside the filter body, the partition plate divides the inside of the filter body into a disinfection chamber and a wastewater chamber from top to bottom, and a filter plate and a filter membrane are fixedly connected inside the disinfection chamber and the wastewater chamber;

[0015] The disinfection chamber and the wastewater chamber are both provided with liquid inlets on the side close to the flushing body, and the liquid inlets are both provided with second one-way valves. The flushing body is provided with liquid outlets at the contact point with the liquid inlet, and the liquid outlets are both provided with third one-way valves.

[0016] Furthermore, a fixing piece is provided above the hanging hole, and the fixing piece includes support rods symmetrically arranged on both sides of the hanging hole and inclined, and a fixed tin foil is fixedly connected to the top of the support rod, and the fixed tin foil is located above the hanging hole.

[0017] Furthermore, the mounting plate is installed obliquely in the flushing body.

[0018] Furthermore, in step 2, the embryo development medium is a solid-liquid biphase medium.

[0019] Further, the solid-liquid biphasic culture medium was prepared as follows: the solid culture medium components were MM3 + 60 g·L-1 sucrose + 0.5 g·L-1 acid hydrolyzed casein + 0.1 g·L-1 inositol + 7 g·L-1 agar + 3 g·L-1 activated carbon;

[0020] The fixed culture medium is covered with a liquid culture medium, the composition of which is WPM + 20 g·L-1 sucrose + 0.2 g·L-16-BA + 0.1 g·L-1 inositol + 7 g·L-1 agar + 1.5 g·L-1 activated carbon.

[0021] Further, in step 2, the composition of the embryo germination medium is 1 / 2MS + 20g·L -1 Sucrose++0.3mg·L -1 IAA+7g·L -1 Agar + 1.5 g L -1 Activated carbon.

[0022] The principles and beneficial effects of the basic scheme are as follows: 1. The breeding of seedless grapes can be achieved through embryo rescue technology, which can quickly breed excellent seedless grape germplasm resources and greatly improve the breeding efficiency. Through this technology, a large number of seedless grape seedlings can be obtained in a short period of time, accelerating the breeding process; embryo rescue technology can induce genetic variation and create new seedless grape varieties. Through appropriate treatment and culture conditions, gene mutation or gene recombination can be induced to produce seedless grape variants with new traits, providing more options for breeding; embryo rescue technology can shorten the breeding cycle and accelerate the selection of new seedless grape varieties. Compared with traditional seedless grape breeding methods, embryo rescue technology can greatly shorten the cultivation time from breeding materials to new varieties and improve breeding efficiency.

[0023] 2. When washing the fruit, first open the closing plate, then pull the mounting plate by hand to make the slider slide horizontally in the slide groove, thereby exposing the mounting plate, and gradually hang the fruit in the hanging holes respectively, and at the same time pinch the tin foil close to fix the tin foil to the branches, thereby supporting the fruit through the hanging holes and the fixing parts, and then put the mounting plate back to its original position and close the closing door;

[0024] At the same time, the nozzle is first turned on, and the nozzle will spray water into the flushing body, thereby realizing the flowing water flushing of the fruit, wherein the water of the nozzle will also flow to the guide hole, and then the water will flow from the guide hole to the guide pipe, wherein because the guide pipe is inclined, the water flowing out of the guide pipe will contact the root of the fruit, and then the root is also fully cleaned, and the cleaned water will flow into the disinfection soaking frame along the guide pipe, and when it needs to be discharged, the first one-way valve and the second one-way valve and the third one-way valve at the waste water chamber are opened, and then the waste water will flow into the waste water chamber to realize the collection of the waste water;

[0025] At the same time, when alcohol soaking is required, all the one-way valves are closed, and alcohol is sprayed from the nozzle, and then the electric control cylinder is started. Under the action of the electric control cylinder, the disinfection soaking frame moves upward and gradually approaches the fruit, and the slider is gradually stuck in the groove. When the amount of alcohol in the disinfection soaking frame reaches a certain amount, the fruit can be soaked in the disinfection soaking frame for a certain period of time. After soaking, the disinfection soaking frame is moved vertically downward, and the first one-way valve and the second and third one-way valves at the disinfection chamber are opened, so that the waste alcohol flows into the disinfection chamber;

[0026] When the fruit needs to be cleaned later, it can be cleaned again according to the above-mentioned cleaning operation. For this reason, through the design of the device, multiple fruits can be cleaned simultaneously in large quantities at one time, and the staff does not need to perform the cleaning operation all the time. The fruit can be cleaned efficiently and quickly only by machinery. At the same time, the waste gas disinfectant and water after cleaning can be collected, which is convenient for their subsequent unified discharge or reuse.

[0027] 3. The water discharged into the disinfection chamber and the wastewater chamber can be filtered again by the filter plate and the filter membrane, thereby intercepting the impurities in the wastewater and waste gas disinfectant, reducing the subsequent discharge of corresponding waste gas substances, and the problem of impurities clogging the pipeline, which can also facilitate its subsequent reuse.

[0028] 4. When washing the fruit, because the mounting plate is designed to be inclined, the washed residues and other impurities can gradually fall into the disinfection soaking frame along the inclined mounting plate, and the impurities in the subsequent disinfection soaking frame will flow into the filter assembly, thereby achieving more sufficient cleaning and disinfection of the fruit clusters, reducing inadequate cleaning or disinfection, and thus affecting the results of subsequent embryo rescue. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 Schematic diagram of the rapid breeding method of seedless grapes in an embodiment of the present invention.

[0030] Figure 2 It is a front view of a flushing device in the rapid seedless grape breeding method according to an embodiment of the present invention.

[0031] Figure 3 It is a side view of a flushing device in the rapid seedless grape breeding method according to an embodiment of the present invention.

[0032] Figure 4 It is a side view of a stripper in the rapid seedless grape breeding method according to an embodiment of the present invention.

[0033] Figure 5 It is a front view of a stripper in the rapid seedless grape breeding method according to an embodiment of the present invention. DETAILED DESCRIPTION

[0034] The following is further described in detail through specific implementation methods:

[0035] The figure marks in the drawings of the specification include: flushing body 1, fixed tin foil 2, support rod 201, hanging hole 3, guide hole 4, guide tube 5, opening 6, disinfection immersion frame 7, electric control cylinder 8, first one-way valve 9, filter body 10, wastewater chamber 11, filter membrane 12, filter plate 13, partition plate 14, disinfection chamber 15, second one-way valve 16, third one-way valve 17, mounting plate 18, nozzle 19, slide groove 20, slider 21, groove 22, handle 23, airbag 24, pressing frame 25, stripping sheet 26, disinfectant tube 27, stripping teeth 28, handrail ring 29.

[0036] Example 1

[0037] The difference from the above embodiment is that a method for rapid breeding of seedless grapes comprises the following steps:

[0038] Step 1, select the parents and hybridize: select seedless excellent varieties as the female parent and the male parent respectively, and use tweezers to manually emasculate the inflorescence of the female parent, and bag and mark it. When transparent mucus appears on the stigma 2-3 days after emasculation, artificial pollination is carried out; pollination needs to be carried out continuously for 2-3 times, with an interval of 24 hours each time;

[0039] Step 2, embryo rescue: cut the hybridized ears and retrieve them, and rinse the fruits with running water for 2-4 hours, transfer the fruits to bottles for disinfection, and use a peeler to peel the ovules, and inoculate the removed ovules on embryo development medium for dark culture. After 8-10 weeks, dissect the embryos and inoculate them on embryo germination medium for light culture;

[0040] like Figure 4-Figure 5 As shown, the stripper includes a handle 23, a stripping piece 26 is fixedly connected to the top of the handle 23, a plurality of stripping teeth 28 are provided on one side of the stripping piece 26, the top of the stripping piece 26 is sharp, and a handrail ring 29 is provided on one side of the lower part of the handle 23, an air bag 24 is also provided inside the handle 23, the air bag 24 is filled with disinfectant, a pressing frame 25 corresponding to the position of the air bag 24 is provided on one side of the handle 23, the air bag 24 is connected to a disinfectant tube 27, the disinfectant tube 27 passes through the handle 23 and extends into the stripping piece 26, a disinfectant hole is provided on the top of the stripping piece 26, and a silicone cross valve is provided on the disinfectant tube 27.

[0041] The embryo development medium is a solid-liquid biphase medium, and the preparation of the solid-liquid biphase medium is as follows: the solid culture medium component is MM3+60g·L -1 Sucrose + 0.5 g·L -1 Acid hydrolyzed casein + 0.1g·L -1 Inositol +7g·L -1 Agar + 3 g L -1 Activated carbon;

[0042] Cover the fixed culture medium with liquid culture medium, the composition of which is WPM+20g·L -1 Sucrose + 0.2 g L -1 6-BA+0.1g·L -1 Inositol +7g·L -1 Agar + 1.5 g L -1 Activated carbon;

[0043] The composition of embryo germination medium is 1 / 2MS + 20g·L -1 Sucrose++0.3mg·L -1 IAA+7g·L -1 Agar + 1.5 g L -1 Activated carbon.

[0044] Step 3, transplanting and hardening the seedlings: Take out the seedlings after embryo rescue, remove the culture medium, and put them into pots with sterilized matrix, place them in natural sunlight and temperature to harden the seedlings, and transplant them to the field for planting after 2-3 months.

[0045] Among them, Figure 2 and Figure 3 As shown, in step 2, the fruit is rinsed and disinfected by using a flushing device, which includes a flushing body 1, an opening 6 is provided on one side of the flushing body 1, a closing plate is provided on the opening 6, a plurality of nozzles 19 capable of connecting clean water and disinfectant are provided on the top of the flushing body 1, a mounting plate 18 is provided below the nozzle 19, the mounting plate 18 is located on one side of the opening 6, the mounting plate 18 is obliquely installed in the flushing body 1, sliders 21 are provided on both sides of the mounting plate 18, a slide groove 20 corresponding to the position of the slide groove 21 is provided on the inner wall of the flushing body 1, and the mounting plate 18 and the flushing body 1 are laterally slidably matched through the slide groove 20 of the slide 21;

[0046] A plurality of hanging holes 3 are provided on the mounting plate 18, and guide holes 4 are symmetrically provided on both sides of each hanging hole 3, and an oblique guide tube 5 is fixedly connected to the bottom of each guide hole 4, and the guide tube 5 is inclined toward one side of the hanging hole 3; a fixing piece is provided above each hanging hole 3, and the fixing piece includes a support rod 201 symmetrically arranged on both sides of the hanging hole 3 and inclined, and a fixed tin foil 2 is fixedly connected to the top of the support rod 201, and the fixed tin foil 2 is located above the hanging hole 3;

[0047] A disinfection soaking frame 7 is provided below the mounting plate 18, an electric control cylinder 8 is fixedly connected to the bottom of the disinfection soaking frame 7, the cylinder body of the electric control cylinder 8 is fixedly connected to the inner bottom wall of the flushing body 1, grooves 22 corresponding to the position of the slider 21 are provided on both side walls of the disinfection soaking frame 7, a flow port is provided on one side of the disinfection soaking frame 7, and a first one-way valve 9 is provided on the flow port;

[0048] A filter assembly is also provided on one side of the flushing body 1, and the filter assembly includes a filter body 10, and a partition plate 14 is fixedly connected inside the filter body 10, and the partition plate 14 divides the inside of the filter body 10 from top to bottom into a disinfection chamber 15 and a wastewater chamber 11, and a filter plate 13 and a filter membrane 12 are fixedly connected in both the disinfection chamber 15 and the wastewater chamber 11;

[0049] The disinfection chamber 15 and the wastewater chamber 11 are both provided with liquid inlets on the side close to the flushing body 1, and the liquid inlets are both provided with second one-way valves 16. The flushing body 1 is provided with liquid outlets at the contact point with the liquid inlets, and the liquid outlets are both provided with third one-way valves 17.

[0050] The specific implementation process is as follows: In step 2, the fruit cleaning and ovule stripping process is as follows: first, when the fruit is rinsed and disinfected, the rinsing device is first moved to the clean bench and the closing plate is opened, and then the mounting plate 18 is pulled by hand to make the slider 21 slide horizontally in the slide groove 20, so that the mounting plate 18 is exposed, and the fruits are gradually hung in the hanging holes 3 respectively, and the tin foil is pinched to fix the tin foil and the branches, and the nozzle 19 is turned on, and the nozzle 19 will spray sterile water into the rinsing body 1, so as to achieve the fruit The fruit is rinsed with flowing water, wherein the water from the nozzle 19 will also flow to the guide hole 4, and then the water will flow from the guide hole 4 to the guide pipe 5, and the water flowing out of the guide pipe 5 will contact the root of the fruit, and then the root is also fully cleaned, and the cleaned water will flow into the disinfection soaking frame 7 along the guide pipe 5. When it needs to be discharged, the first one-way valve 9 and the second one-way valve 16 and the third one-way valve 17 at the waste water chamber 11 are opened, and then the waste water will flow into the waste water chamber 11 to realize the collection of the waste water;

[0051] When alcohol soaking is required, firstly, disinfection gas is input into the flushing body 1 to fumigate the inside of the flushing body 1. After the disinfection is completed, all the one-way valves are closed, and alcohol is sprayed from the nozzle 19. Then, the electric control cylinder 8 is started. Under the action of the electric control cylinder 8, the disinfection soaking frame 7 moves upward and gradually approaches the fruit. At the same time, the slider 21 is also gradually stuck in the groove 22. When the amount of alcohol in the disinfection soaking frame 7 reaches a certain amount, the fruit can be soaked in the disinfection soaking frame 7 for a certain period of time. After the soaking is completed, the disinfection soaking frame 7 is moved vertically downward, and the first one-way valve 9 and the second one-way valve 16 and the third one-way valve 17 at the disinfection chamber 15 are opened, so that the waste alcohol flows into the disinfection chamber 15.

[0052] Then sterile water needs to be injected again to clean the fruit again, and the fruit is cleaned again according to the above cleaning method. After cleaning, the waste water is discharged again. By placing the device on the clean bench for operation and flushing with sterile water, it can be ensured that the fruit is less contaminated during the cleaning process.

[0053] Finally, when stripping the ovules, first place the stripper in a specially sterilized box for sterilization, then move the sterilized stripper to the clean bench and close to the ovules, wherein the stripper needs to be constantly placed on an alcohol lamp for intermittent heating and sterilization during use, then use the stripping teeth 28 to cut the skin of the cleaned fruit, and after cutting, use the sharp end of the stripping piece 26 to pick out the ovules, and at the same time, when picking out the ovules, the air bag 24 at the pressing frame 25 can be pressed by hand to expose it, and then the silicone cross valve can be opened under the change of pressure, and then the disinfectant flows into the disinfectant pipe 27, and then flows out from the disinfectant hole, and then the picked ovules can be preliminarily disinfected, reducing the contamination of the ovules during the ovule picking process, thereby affecting the subsequent breeding effect;

[0054] After the ovules are peeled out and disinfected, they should be soaked in sterile water, rinsed and dried for use in the next experiment.

[0055] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0056] The above is only an embodiment of the present invention. The common sense such as the known specific structure and characteristics in the scheme is not described in detail here. The ordinary technicians in the relevant field know all the common technical knowledge in the technical field of the invention before the application date or priority date, can obtain all the existing technologies in the field, and have the ability to apply the conventional experimental means before that date. The ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A method for rapid seedless grape breeding, characterized in that: The following steps are involved: Step 1, select the parents and hybridize: select seedless excellent varieties as the female parent and the male parent respectively, and use tweezers to manually emasculate the inflorescence of the female parent, and mark it with a bag. When transparent mucus appears on the stigma 2-3 days after emasculation, artificial pollination is performed; Step 2, embryo rescue: cut the hybridized ears and retrieve them, and rinse the fruits with running water for 2-4 hours, transfer the fruits to bottles for disinfection, and use a peeler to peel the ovules, and inoculate the removed ovules on embryo development medium for dark culture. After 8-10 weeks, dissect the embryos and inoculate them on embryo germination medium for light culture; Step 3, transplanting and hardening the seedlings: Take out the seedlings after embryo rescue, remove the culture medium, and put them into pots with sterilized matrix, place them in natural sunlight and temperature to harden the seedlings, and transplant them to the field for planting after 2-3 months.

2. The method for rapid seedless grape breeding according to claim 1, characterized in that: In step 1, pollination needs to be carried out continuously for 2-3 times, with an interval of 24 hours between each pollination.

3. The method for rapid seedless grape breeding according to claim 2, characterized in that: In step 2, the fruit is rinsed and disinfected by a rinsing device, which includes a rinsing body, an opening is provided on one side of the rinsing body, a closing plate is provided on the opening, a plurality of nozzles capable of connecting clean water and disinfectant are provided on the top of the rinsing body, a mounting plate is provided below the nozzle, the mounting plate is located on one side of the opening, sliders are provided on both sides of the mounting plate, a sliding groove corresponding to the position of the slider is provided on the inner wall of the rinsing body, and the mounting plate and the rinsing body are laterally slidably matched through the sliding groove of the slider; The mounting plate is provided with a plurality of hanging holes, each of which is symmetrically provided with guide holes on both sides, and the bottom of each guide hole is fixedly connected with an oblique guide tube, which is inclined toward one side of the hanging hole; A disinfection soaking frame is provided under the mounting plate, an electric control cylinder is fixedly connected to the bottom of the disinfection soaking frame, the cylinder body of the electric control cylinder is fixedly connected to the inner bottom wall of the flushing body, grooves corresponding to the position of the slider are provided on both side walls of the disinfection soaking frame, a flow port is provided on one side of the disinfection soaking frame, and a first one-way valve is provided on the flow port.

4. The method for rapid seedless grape breeding according to claim 3, characterized in that: A filter assembly is also provided on one side of the flushing body, and the filter assembly includes a filter body, and a partition plate is fixedly connected inside the filter body, and the partition plate divides the inside of the filter body into a disinfection chamber and a wastewater chamber from top to bottom, and the disinfection chamber and the wastewater chamber are both fixedly connected with a filter plate and a filter membrane; The disinfection chamber and the wastewater chamber are both provided with liquid inlets on the side close to the flushing body, and the liquid inlets are both provided with second one-way valves. The flushing body is provided with liquid outlets at the contact point with the liquid inlet, and the liquid outlets are both provided with third one-way valves.

5. The method for rapid seedless grape breeding according to claim 4, characterized in that: A fixing piece is arranged above the hanging hole, and the fixing piece comprises a supporting rod symmetrically arranged on both sides of the hanging hole and inclined, and a fixing tin foil is fixedly connected to the top of the supporting rod, and the fixing tin foil is located above the hanging hole.

6. The method for rapid seedless grape breeding according to claim 5, characterized in that: The mounting plate is installed obliquely into the flushing body.

7. The method for rapid seedless grape breeding according to claim 6, characterized in that: In step 2, the embryo development medium is a solid-liquid biphase medium.

8. The method for rapid seedless grape breeding according to claim 7, characterized in that: The preparation of solid-liquid biphasic culture medium is as follows: the solid culture medium component is MM3+60g·L -1 Sucrose + 0.5 g·L -1 Acid hydrolyzed casein + 0.1g·L -1 Inositol +7g·L -1 Agar + 3 g L -1 Activated carbon; Cover the fixed medium with liquid culture medium, the liquid culture medium composition is WPM + 20g·L -1 Sucrose + 0.2 g·L -1 6-BA+0.1g·L -1 Inositol +7g·L -1 Agar + 1.5 g L -1 Activated carbon.

9. The method for rapid seedless grape breeding according to claim 8, characterized in that: In step 2, the composition of the embryo germination medium is 1 / 2MS + 20g·L -1 Sucrose++0.3mg·L -1 IAA+7g·L -1 Agar + 1.5 g L -1 Activated carbon.

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