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Culture medium box set and method for in-vitro rapid propagation of scaevola taccada

A medium and inducing medium technology, applied in the field of plant tissue culture, can solve problems such as unseen reproduction, and achieve the effects of good growth and increased reproduction rate.

Active Publication Date: 2019-01-04
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Abstract
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Problems solved by technology

[0003] At present, the main propagation methods of Pittonia pratense are seed propagation and cutting propagation, and there is no report of realizing its propagation by means of plant tissue culture.

Method used

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  • Culture medium box set and method for in-vitro rapid propagation of scaevola taccada

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Embodiment 1

[0063] 1. Establishment of Pittosporum aseptic system and induction of clustered buds

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Abstract

The invention discloses a culture medium box set and method for in-vitro rapid propagation of scaevola taccada. The culture medium box set for in-vitro rapid propagation of scaevola taccada comprisesa scaevola taccada cluster bud induction culture medium, a somatic embryo and adventitious bud induction culture medium, a somatic embryo and adventitious bud proliferation and elongation culture medium, a scaevola taccada rooting induction culture medium and river sand used as a scaevola taccada tissue culture seedling transplanting medium. Moreover, an efficient scaevola taccada tissue culture seedling rapid propagation technology system utilizing the box set is also built. A basis is provided for protecting, developing and utilizing scaevola taccada resources, and a blank of scaevola taccada in the aspect of the tissue culture rapid propagation technology is filled in.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium kit and a method for in vitro rapid propagation of Pittonia chinensis. Background technique [0002] Scaevola taccada is a semi-mangrove plant of the erect or spreading shrubs of the genus Scaevola in the family Goodeniaceae. There are 11 genera and 400 species in the whole family. Almost all origins and existing species are mostly concentrated in Australia, and only Pittosporum is widely distributed all over the world. There are 130 species of Pittosporum, and only 40 species are distributed outside Australia, most of which are scattered in tropical island areas. There are two species in the southern coast of my country, namely Scaevola hainanensis Hance and Scaevola hainanensis Hance, which are mainly distributed on the open seaside sandy land or coastal cliffs in Taiwan, Fujian, Guangdong, Guangxi and Hainan. As a dominant species in...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁韩枝马国华曾宋君吴坤林郑枫张新华熊玉萍任海简曙光张城杨松
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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