Preparation and application of continuous cropping peanut resistant complex bacteria
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A compound inoculant and continuous cropping technology of peanuts, applied in the application, biocide, fungicide and other directions, can solve problems such as serious disease occurrence and environmental pollution, and achieve the effects of preventing and controlling plant diseases, simple use and good stability.
Active Publication Date: 2015-07-01
QINGDAO AGRI UNIV +1
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[0005] Aiming at the problems of environmental pollution and serious disease occurrence in chemical pesticide prevention and control in the prior art, the purpose of the present invention is to provide a biocontrol bacterial compound agent that can effectively decompose peanut allelochemical-degrading bacteria and resist various pathogenic bacteria, It also involves its application in peanut resistance to continuous cropping
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Embodiment 1
[0030] (1) Source of bacteria
[0031] The soil was collected in a peanut field in Shandong Province where peanuts have been continuously planted for many years. The soils were soils of 1 year, 2 years, 4 years, and 7 years. No. 16. The soil sample of the peanut root of the sample is brushed into a ziplock bag, and the soil within 2mm around the root system is collected, total The collected soil weighs about 500g, and it is used for the screening and determination of microorganisms and the isolation and identification of allelochemical substances in cold storage.
[0032] (2) Strain screening
[0033] Nutrient agar medium (NA medium): peptone 5.0g; beef extract 3.0g; glucose 2.5g; yeast powder 1.0g; agar powder 16.0-18.0g; distilled water 1000ml; pH7.0-7.2. Prepare NA liquid medium separately. Martin's agar medium: glucose 10g, peptone 5g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O0.5g, 1 / 3000 Bengal Red 100mL, agar 15-20g, pH natural, distilled water 800mL, add 0.03% streptomycin...
Embodiment 2
[0046] (1) Source of bacteria
[0047] The strain source is with embodiment 1.
[0048] (2) Strain screening
[0049] Culture medium is the same as embodiment 1.
[0050] Screening steps: Take 10 g of fresh soil samples and add them to 100 ml of CM medium containing 2% oleic acid, 1‰ phthalic acid, and 2% hexadecanoic acid respectively, and culture on a shaker at 30°C for 72 hours at 170r / min. Subculture three times, inoculate 100 μl culture solution on NA plate, incubate at 30°C for 24 hours, then randomly pick a single colony according to the different morphology of the colony, purify it for 3 generations, and store it until its degradation efficiency is determined.
[0051] After inoculation in CM medium supplemented with 1 g / L allelochemicals, cultured on a shaker at 170 r / min at 30°C for 7 days, the treatment without inoculation was used as the control, and each treatment was repeated 3 times. Extract 3 times with 1:1 mixed extract of ethanol and ether, combine the ext...
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Abstract
The invention discloses preparation and application of continuous cropping peanut resistant complex bacteria. The complex bacteria comprise continuous cropping peanut resistant bio-control bacteria and an allelochemicals degrading bacteria agent. By using the complex bacteria, the degradation efficiency of the continuous cropping peanut allelochemicals is high, the disease of continuous cropping peanuts can be effectively inhibited, and the using amount of chemical pesticides and the accumulation of the continuous cropping peanut in the soil can be reduced; in addition, the complex bacteria are significant in environment pollution; because the degraded product can be used as a carbon source by the bacteria and is converted into a harmless substance, and the carbon source of the soil is increased, so that the absorption of the crop root system to degradable substances is facilitated, and the pollution to the environment is avoided; the complex bacteria is high in stability; gene mutation is avoided after continuous culturing, the inheritance can be stabilized, the quantity of beneficial bacteria in the soil can be increased, and the soil fertility is increased; moreover, the preparation method of the complex bacteria is simple and an extraction process of the complex bacteria is simple; in addition, toxic substances are not added in the preparation process, and the complex bacteria are easy to use; the quality and the yield of crops such as peanuts in China can be improved.
Description
technical field [0001] The invention belongs to the technical field of biological control compound bacteria agents, and in particular relates to the preparation and application of a peanut continuous cropping-resistant compound bacteria agent, that is, the preparation and application of an anti-peanut continuous cropping allelochemical substance-degrading bacteria and biocontrol bacteria compound bacteria agent. Background technique [0002] As we all know, peanut is one of the important oil crops. China is the largest producer of peanut. The total planting area accounts for 20.4% of the world's total area, and the total output accounts for 40.8%. 17 million tons. The continuous cropping peanut plants are short and poor in growth, the yield and quality are reduced, and the pests and diseases are aggravated. After 3 years of continuous cropping, the yield reduction range is 10% to 40%. According to Zhang Sisu et al.'s many years of experiments and investigation results, cont...
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