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Preparation and application of an anti-peanut continuous cropping compound bacterial agent

A compound bacterial agent and peanut continuous cropping technology, applied in the direction of application, biocide, fungicide, etc., can solve the problems of serious disease occurrence, environmental pollution, etc., achieve the effects of preventing and controlling plant diseases, easy to use, and improving soil fertility

Active Publication Date: 2017-09-22
QINGDAO AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of environmental pollution and serious disease occurrence in chemical pesticide prevention and control in the prior art, the purpose of the present invention is to provide a biocontrol bacterial compound agent that can effectively decompose peanut allelochemical-degrading bacteria and resist various pathogenic bacteria, It also involves its application in peanut resistance to continuous cropping

Method used

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  • Preparation and application of an anti-peanut continuous cropping compound bacterial agent
  • Preparation and application of an anti-peanut continuous cropping compound bacterial agent
  • Preparation and application of an anti-peanut continuous cropping compound bacterial agent

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Source of bacteria

[0031] The soil was collected in a peanut field in Shandong Province where peanuts have been continuously planted for many years. The soils were soils of 1 year, 2 years, 4 years, and 7 years. No. 16. Brush the soil sample from the peanut root into a ziplock bag with a brush, and collect the soil within 2mm around the root system. The total soil weight is about 500g, and it will be stored in cold storage for the screening of microorganisms and the isolation and identification of allelochemicals.

[0032] (2) Strain screening

[0033] Nutrient agar medium (NA medium): peptone 5.0g; beef extract 3.0g; glucose 2.5g; yeast powder 1.0g; agar powder 16.0-18.0g; distilled water 1000ml; pH7.0-7.2. Prepare NA liquid medium separately. Martin's agar medium: glucose 10g, peptone 5g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O0.5g, 1 / 3000 Bengal Red 100mL, agar 15-20g, pH natural, distilled water 800mL, add 0.03% streptomycin diluent 100mL before use, so that each...

Embodiment 2

[0046] (1) Source of bacteria

[0047] The strain source is with embodiment 1.

[0048] (2) Strain screening

[0049] Culture medium is the same as embodiment 1.

[0050] Screening steps: Take 10 g of fresh soil samples and add them to 100 ml of CM medium containing 2% oleic acid, 1‰ phthalic acid, and 2% hexadecanoic acid respectively, and culture on a shaker at 30°C for 72 hours at 170r / min. Subculture three times, inoculate 100 μl culture solution on NA plate, incubate at 30°C for 24 hours, then randomly pick a single colony according to the different morphology of the colony, purify it for 3 generations, and store it until its degradation efficiency is determined.

[0051] After inoculation in CM medium supplemented with 1 g / L allelochemicals, cultured on a shaker at 170 r / min at 30°C for 7 days, the treatment without inoculation was used as the control, and each treatment was repeated 3 times. Extract 3 times with 1:1 mixed extract of ethanol and ether, combine the ext...

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Abstract

The invention discloses the preparation and application of an anti-peanut continuous cropping compound bacterial agent. The compound bacterial agent includes an anti-continuous peanut cropping bacterial agent and an allelochemical degradation bacterial agent. The compound bacterial agent of the present invention has high degradation efficiency for peanut continuous cropping allelochemical substances , can effectively inhibit continuous cropping peanut diseases, reduce the amount of chemical pesticides and their accumulation in the soil, which is extremely important for environmental pollution, and the degraded products can be converted into harmless substances by bacteria as carbon sources, increasing soil carbon sources, which is beneficial The absorption of degraded substances by crop roots will not pollute the environment; the compound bacterial agent has good stability, no gene mutation will occur after continuous cultivation, stable genetics, can increase the number of beneficial bacteria in the soil, improve soil fertility, and The preparation method of the composite bacterial agent of the invention is simple, the extraction process is simple, no toxic substances are added in the preparation process, and the use is simple. It is conducive to improving the quality and output of crops such as peanuts in our country.

Description

technical field [0001] The invention belongs to the technical field of biological control compound bacteria agents, and in particular relates to the preparation and application of a peanut continuous cropping-resistant compound bacteria agent, that is, the preparation and application of an anti-peanut continuous cropping allelochemical substance-degrading bacteria and biocontrol bacteria compound bacteria agent. Background technique [0002] As we all know, peanut is one of the important oil crops. China is the largest producer of peanut. The total planting area accounts for 20.4% of the world's total area, and the total output accounts for 40.8%. 17 million tons. The continuous cropping peanut plants are short and poor in growth, the yield and quality are reduced, and the pests and diseases are aggravated. After 3 years of continuous cropping, the yield reduction range is 10% to 40%. According to Zhang Sisu et al.'s many years of experiments and investigation results, cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P3/00C09K17/00
Inventor 崔德杰赵亚慧咸洪泉辛言君王丽宁
Owner QINGDAO AGRI UNIV
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