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Method for cultivating Monascus strain for highly yielding Monacolin K

A cultivation method and technology of Monascus, which are applied in the field of cultivating high-yield Monacolin K strains of Monascus, can solve the problems of small increase in yield, inability to achieve, and low yield of Monacolin K, etc. Effects of reproductive rate

Inactive Publication Date: 2018-06-15
吕玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, physical mutagenesis and chemical mutagenesis are commonly used in solid-state fermentation and liquid-state fermentation in the production of Monascus to achieve the production of Monascus strains. Although the existing mutagenesis methods can increase the yield of strains, they can increase the yield on the original yield. Small, unable to reach the quantity required by the existing market, and because the yield of Monascus strains is not high, the yield of Monacolin K is low

Method used

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  • Method for cultivating Monascus strain for highly yielding Monacolin K
  • Method for cultivating Monascus strain for highly yielding Monacolin K

Examples

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Effect test

Embodiment 1

[0019] A method for cultivating a high-yield monascus K strain, comprising the following steps:

[0020] 1) The preparation of Monascus strains includes the following specific steps: A. Separation and purification, first mix red yeast rice with distilled water in a ratio of 3:1, and make rice milk through a grinding machine, and then filter through a filter to obtain a bacterial suspension , standby; B, get the bacterium suspension that makes in concrete step A and drop in the red yeast rice multiplication medium that is made by soybean juice, cultivate 5 days in the incubator of 30 ℃, carry out by 10% inoculum again Second multiplication and cultivation for 5 days, select the purified Monascus strain, if it is not pure, you can carry out the third multiplication and cultivation for 5 days according to 10% inoculum size, and so on, until the purified Monascus strain is isolated and used for future use .

[0021] 2) Incline culture of Monascus colony, its concrete steps are: A...

Embodiment 2

[0029] A method for cultivating a high-yield monascus K strain, comprising the following steps:

[0030] 1) The preparation of Monascus strains includes the following specific steps: A. Separation and purification, first mix red yeast rice with distilled water in a ratio of 3:1, and make rice milk through a grinding machine, and then filter through a filter to obtain a bacterial suspension , for subsequent use; B, get the bacterium suspension that makes in concrete step A and drip in the red yeast rice multiplication medium that is made by soybean juice, cultivate 7 days in the incubator of 30 ℃, carry out by 10% inoculum again Second multiplication and cultivation for 7 days, select the purified Monascus strain, if it is not pure, you can carry out the third multiplication and cultivation for 7 days according to 10% inoculum size, and so on, until the purified Monascus strain is isolated and used for future use .

[0031] 2) Incline culture of Monascus colony, its concrete s...

Embodiment 3

[0039] A method for cultivating a high-yield monascus K strain, comprising the following steps:

[0040] 1) The preparation of Monascus strains includes the following specific steps: A. Separation and purification, first mix red yeast rice with distilled water in a ratio of 3:1, and make rice milk through a grinding machine, and then filter through a filter to obtain a bacterial suspension , standby; B, get the bacterium suspension that makes in concrete step A and drop in the red yeast rice proliferation medium that is made by soybean juice, cultivate 6 days in the incubator of 30 ℃, carry out by 10% inoculum again Second multiplication and cultivation for 6 days, select the purified Monascus strain, if it is not pure, you can carry out the third multiplication and cultivation for 6 days according to 10% inoculum size, and so on, until the purified Monascus strain is isolated and used for future use .

[0041] 2) Incline culture of Monascus colony, its concrete steps are: A,...

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Abstract

The invention discloses a method for cultivating a Monascus strain for highly yielding Monacolin K. The method comprises the following steps: separating and purifying red yeast rice to obtain a Monascus colony, adding the prepared Monascus colony to a medium prepared from agar, a potato juice, a mallet juice, lactic acid, anhydrous ethanol, sodium nitrate and potassium dihydrogen phosphate to prepare Monascus spores, flushing the medium with sterile sodium chloride water to obtain a Monascus spore bacterium suspension, performing ultraviolet irradiation on the Monascus spore bacterium suspension to achieve physical mutagenesis, adding a diethyl sulfate solution to achieve further mutagenesis, and mixing the obtained Monascus spore bacterium suspension with rice flour to rapidly breed the Monascus strain. An atomic irradiation mutagenesis technology is combined with a high-temperature piling cultivation technology, so the breeding rate of the Monascus strain in the medium is effectivelyincreased, the number of the Monascus strain is increased, and the metabolic Monacolin K is also increased, thereby the yield of the Monacolin K is increased.

Description

technical field [0001] The invention relates to a method for cultivating a monascus K strain with high yield. Background technique [0002] Biopharmaceutical is an industry that the state encourages development during the 12th Five-Year Plan period. Currently, cancer has become a major threat to human health around the world. The main treatment methods are based on Western medicine, including surgery, radiotherapy and chemotherapy, etc., all of which have strong side effects. However, until now, cancer can only be controlled by drugs. Compared with the previous anti-cancer drugs, the newly developed anti-cancer functional red yeast rice can maintain a higher blood concentration in the body, thereby improving the anti-cancer activity . And there is no drug toxicity, convenient administration, only oral administration, patients do not need to be hospitalized, and the quality of life is improved. Anti-cancer functional red yeast rice has remarkable curative effect, and it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/01C12R1/645
CPCC12N1/14C12N15/01
Inventor 吕玲
Owner 吕玲
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