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Tissue culture rapid propagation method of Abelmoschus manihot

A technology of tissue culture rapid propagation and hollyhock, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low seedling emergence rate in seed propagation, and achieve the effects of short cultivation period, simple operation and high yield

Inactive Publication Date: 2010-06-02
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in terms of production, since the seed leather of hollyhock seeds is hard, the seedling emergence rate of seed propagation is very low. How to improve the reproduction coefficient has become a key technology to be solved in the supply of hollyhock seeds.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The operation steps of the present embodiment are as follows: a, get the hollyhock seeds and disinfect them with 75% ethanol for 1min on the ultra-clean workbench, 0.1%Hgcl 2 Sterilize for 10 minutes, rinse with sterile water 5 times;

[0014] b. Insert the treated seeds in step a into medium MS at a temperature of 25±1° C., light of 1500 LX, and time of 12 h / d for germination;

[0015] c. Cut the stems of the seedlings with a seedling age of 25 days in step b into 1.5cm segmented stems and insert them into the medium MS+6-BA1.5mg / L+NAA0.15mg / L, and place them at a temperature of 25±1°C. The light is 2000LX, and the time is 14h / d for cultivation, and the differentiation coefficient reaches 10.3;

[0016] d. Cut off the 2.0cm axillary buds in step c, insert them into the culture medium 1 / 2MS+IBA0.6mg / L and place them at a temperature of 25±1°C, light of 2000LX, and time of 14h / d to induce rooting. Rate 100%.

Embodiment 2

[0018] The operating steps of the present embodiment are as follows: a, get the hollyhock seeds and disinfect them with 75% ethanol for 1.5min on the ultra-clean workbench, 0.1%Hgcl 2 Sterilize for 9 minutes, wash with sterile water 4 times;

[0019] b. Insert the treated seeds in step a into the culture medium MS at a temperature of 25±1° C., light of 1800 LX, and time of 14 h / d for germination;

[0020] c. Cut the 40-day-old seedling stems in step b into 1cm segmented stems and insert them into medium MS+6-BA1.0mg / L+NAA0.1mg / L, place them at a temperature of 25±1°C, and light 1500LX, culture time 14h / d, the differentiation coefficient reached 9.6;

[0021] d. Cut off the 2.0cm axillary buds in step c, insert them into the culture medium 1 / 2MS+IBA0.4mg / L and place them at a temperature of 25±1°C, light of 2000LX, and time of 12h / d to induce rooting. rate reached 100%.

Embodiment 3

[0023] The operation steps of the present embodiment are as follows: a, get the hollyhock seeds and disinfect them with 75% ethanol for 1min on the ultra-clean workbench, 0.1%Hgcl 2 Sterilize for 10 minutes, rinse with sterile water 5 times;

[0024] b. Insert the treated seeds in step a into the culture medium MS at a temperature of 25±1° C., light of 1500 LX, and time of 10 h / d for germination;

[0025] c. Cut the 50d seedling stems in step b into 2cm segmented stems and insert them into the medium MS+6-BA1.5mg / L+NAA0.2mg / L, place at a temperature of 25±1°C, and light 2000LX, cultured for 12h / d, the differentiation coefficient reached 10.2;

[0026] d. Cut off the 2.5cm axillary buds in step c, insert them into the culture medium 1 / 2MS+IBA0.5mg / L, and place them at a temperature of 25±1°C, light of 2000LX, and time of 14h / d to induce rooting. rate reached 100%.

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Abstract

The invention discloses a tissue culture rapid propagation method of Abelmoschus manihot, a plant of Abelmoschus in Malvaceae. The method comprises the three steps of obtaining sterile sprouts from seeds, multiplying the sprouts and inducing the sprouts to root so as to obtain a large quantity of Abelmoschus manihot seedlings in short time, wherein the step of multiplying sprouts is described as follows: cutting the stem of each sterile sprout in the seedling age of 25-50d into 1-2 cm stem sections with knots, implanting the stem sections into a culture medium of MS+6-BA1.0-1.5mg / L+NAA0.1-0.2mg / L, and culturing the stem sections under certain condition of temperature and sun lighting. The step of inducing sprouts to root is described as follows: cutting 1.0-2.0cm auxiliary buds obtained in the step of multiplying sprouts, implanting the auxiliary buds into a culture medium of 1 / 2MS+IBA0.4-0.6mg / L, and inducing the auxiliary buds to root under certain temperature and sun lighting. The invention has the advantages of simple method, high multiplying speed and stability, short culturing cycle and high yield. With higher feasibility in actual production and application, the invention is suitable for the industrialization production of Abelmoschus manihot.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of hollyhock, a plant of the genus okra in the Malvaceae family, and belongs to the field of forestry biotechnology. Background technique [0002] The whole herb of hollyhock can be used as medicine. The flower has the effect of clearing away dampness and heat, reducing inflammation and detoxification, and the fruit and seeds can treat indigestion, lack of appetite, traumatic injuries, etc. The use of hollyhock to treat diseases and injuries has been recorded in the medical books of all dynasties in my country, and the folk application is also very common. There is a huge demand market for hollyhock at home and abroad. There is no research on tissue culture and rapid propagation of hollyhock in China. However, in production, since the seed leather of hollyhock seeds is hard, the seedling emergence rate of seed propagation is very low. How to improve the reproduction coefficient...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 胡冬南沈勇根
Owner JIANGXI AGRICULTURAL UNIVERSITY
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