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Method for establishing giant grouper sperm freezer and assisting grouper distant hybridization breeding

A saddle grouper, a technology for establishing a method, applied in the field of fish breeding, can solve the problems of asynchrony of maturation, insufficient semen volume, small number of male fish, etc., and achieve the effects of improving the probability of success and expanding the scope.

Active Publication Date: 2017-02-22
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0006] The invention relates to a method for establishing a sperm freezer of saddletail grouper and a method for assisting grouper distant hybrid breeding with frozen sperm, which solves the problems of small number of male fish, asynchronous maturity and insufficient semen volume in artificial breeding of grouper problem, overcome the reproductive isolation between grouper species caused by factors such as geographical distribution, breeding time, and breeding temperature, thereby realizing the distant hybrid breeding between different species of grouper, and cultivating species with excellent growth characteristics. New species of grouper

Method used

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  • Method for establishing giant grouper sperm freezer and assisting grouper distant hybridization breeding
  • Method for establishing giant grouper sperm freezer and assisting grouper distant hybridization breeding
  • Method for establishing giant grouper sperm freezer and assisting grouper distant hybridization breeding

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Embodiment 1

[0055] 1. Establishment of a frozen storage for grouper grouper sperm

[0056] (1) Preparation of sperm diluent (ELRS3): use glucose 49.97g / L, NaCl 6.5g / L, NaHCO 3 0.199g / L, KCl 0.14g / L, CaCl 2 ·2H 2 O 0.12g / L, NaH 2 PO 4 0.0098g / L, the sperm dilution ELRS3 is prepared in distilled water, the osmotic pressure is 400mOsm / L, and the pH is 7.03.

[0057] (2) Preparation of sperm cryopreservation solution: Add 15% dimethyl sulfoxide (DMSO) and 10% calf serum (FBS) into sperm dilution ELRS3 to prepare a sperm cryopreservation solution, which should be placed in a refrigerator at 4°C before use. , Are all parts by volume.

[0058] (3) Sperm cryopreservation: during the sexual maturity season of grouper grouper (April to July each year), the sperm of grouper grouper is carried out at the Lingshui grouper farm in Sanya, Hainan and Laizhou Mingbo Aquatic Products Co., Ltd. in Shandong Province Cryopreservation, the sexually mature grouper is anesthetized with 50ppm eugenol, and the sperm is ...

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Abstract

The invention discloses a method for establishing a giant grouper sperm freezer and assisting grouper distant hybridization breeding. The sperm freezer establishing method comprises the steps of preparing sperm diluent, preparing sperm cryopreservation liquid, freezing and storing sperms and establishing the sperm freezer. The grouper distant hybridization breeding method comprises the steps of establishing the giant grouper sperm freezer, establishing saladfish breeding groups, cultivating saladfish parent fish and performing reproductive regulation, performing artificially spawning breeding, fertilizing the frozen sperms and performing incubation, and cultivating fries. According to the method, the first giant grouper sperm freezer is established in China, reproductive isolation of the groupers caused by factors, such as geographical distribution, optimum temperature range and breeding time, is overcome, and distant hybridization breeding of the grouper groups is possible, so that distant hybridization breeding varieties are cultivated.

Description

Technical field [0001] The invention belongs to the technical field of fish breeding, and in particular relates to a method for establishing a frozen grouper spermatozoa and a method for assisting grouper distant hybridization breeding by using frozen sperm. Background technique [0002] Grouper is an important marine aquaculture fish and excellent germplasm resources in my country. There are 36 species of grouper in China, mainly distributed in the East China Sea and the South China Sea. In 2014, the catch of grouper reached 113,096 tons and the amount of breeding reached 88,130 tons, which plays an important role in the development of blue agriculture. In recent years, with the rapid development of the marine aquaculture industry, this rare fish species has begun to be bred in large quantities in the north and south of my country, and the demand for seedlings is over 100 million. At the same time, the demand for fine varieties of breeding enterprises has further increased. At ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01N1/02
CPCA01K61/00A01N1/0221Y02A40/81
Inventor 田永胜翟介明陈张帆马文辉段会敏唐江刘江春侯云霞庞尊方孙正祥李文升李波
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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