Method of photolyzing seawater by tetraselmis chui to produce hydrogen using fuel cell hydrogen consuming technique

Abstract

The invention relates to a method for preparing hydrogen through water photolysis, which in detail relates to a method for generating hydrogen through seawater photolysis with marine green alga. It takes marine green alga asww systematic hydrogen generation material, installs alkaline hydrogen-oxygen fuel cell continuous hydrogen consuming system on photoreactor to maintain low hydrogen pressure in system, the hydrogen concentration in system can be reflected by measuring the circuit current outside battery according to the relevant relationship between electron number and hydrogen number, and studies the characteristics of green alga under CCCP stress and regulation. The hydrogen generation time is prolonged by 5 times under continuous hydrogen consumption by fule battery to 120 hours; and the total hydrogen output is 54.4 ml, which is 2.2 times of the optimal level of unused fule battery continuous hydrogen consumption system. The invention is characterized by high-effective and sensitive inspection for hydrogen generation, simple method, strong anti-interference and wide application prospect.

Description

technical field

[0001] The invention relates to a method for producing hydrogen by photolysis of water, in particular to a method for producing hydrogen by photolysis of seawater from algae using fuel cell hydrogen consumption technology. Background technique

[0002] The main problem of hydrogen production by green algae is that oxygen severely inhibits hydrogenase. Because hydrogen is produced at the same time as oxygen, if the inhibition of oxygen cannot be eliminated, the rate of hydrogen production is low and hydrogen cannot be produced continuously for a long time. Abroad, a lot of work has been done on the screening of aerobic strains. Scientists from the National Renewable Energy Laboratory (NREL) and the University of California successfully studied the two-step indirect photohydrolysis hydrogen production process of freshwater Chlamydomonas reversible hydrogenase, using the mutant Chlamydomonas reinhardtii CC- 124, achieve O 2 and H 2 The generation of hydrogena...

Examples

Embodiment 1

[0035] Such as figure 1Shown is a schematic diagram of the photobiological hydrogen production system, in which 3 is a photoreactor, made of glass materials, the gas volume change in the photoreactor 3 is collected and measured in the water tank 8 through the calibration gas sampling tube 7, and the consumption of the fuel cell The hydrogen current is automatically detected and recorded by the data acquisition and recording system. The subcardiac flat algae were cultured with Kangweifang nutrient salts. After culturing to the late logarithmic growth stage, they were collected by centrifugation until the concentration of the hydrogen-producing algae liquid was 8 million cells / mL. Using the two-step method of photolysis of water to produce hydrogen from flat algae, take a stirred photohydrogen reactor with a volume of 500mL, fill it with algae liquid, seal the bottle mouth, replace the air with nitrogen for 5 minutes, and place it in a 25°C incubator in the dark. Cultured to i...

Embodiment 2

[0040] The subcardiac flat algae are cultivated with Kangweifang nutrient salts. After culturing to the late logarithmic growth stage, they are centrifuged, collected by membrane filtration, or cultured at high density to a concentration of 6 million cells / mL in the hydrogen-producing algae liquid. The method of producing hydrogen by photolysis of water using two-step method of flat algae, the process is as follows figure 2 Show. Take a stirred-type photohydrogen production reactor with a volume of 500 mL, fill it with algae liquid, seal the bottle mouth, replace the air with nitrogen for 5 min, and place it in a 25°C incubator for dark cultivation to induce reversible hydrogenase. After 20 hours of dark induction to the algae solution, CCCP was added to a concentration of 15 μM before light, and the pH value was adjusted to 8.2 with sodium bicarbonate solution. The system stirs the algae liquid at a medium light intensity of 9000LX and a stirring speed of 150rpm, so that the...

Embodiment 3

[0047] The subcardiac flat algae are cultivated with Kangweifang nutrient salts. After culturing to the late logarithmic growth stage, they are centrifuged, collected by membrane filtration, or cultured at high density to a concentration of 6 million cells / mL in the hydrogen-producing algae liquid. The method of producing hydrogen by photolysis of water using two-step method of flat algae, the process is as follows figure 2 Show. Take a stirred-type photohydrogen production reactor with a volume of 500 mL, fill it with algae liquid, seal the bottle mouth, replace the air with nitrogen for 5 min, and place it in a 25°C incubator for dark cultivation to induce reversible hydrogenase. After 20 hours of dark induction, CCCP was added to the concentration of 7.5 μM before light, and the pH value was adjusted to 8.2 with sodium bicarbonate solution. The system stirs the algae liquid at a medium light intensity of 9000LX and a stirring speed of 150rpm, so that the algae liquid in th...

technical field

[0001] The invention relates to a method for producing hydrogen by photolysis of water, in particular to a method for producing hydrogen by photolysis of seawater from algae using fuel cell hydrogen consumption technology. Background technique

[0002] The main problem of hydrogen production by green algae is that oxygen severely inhibits hydrogenase. Because hydrogen is produced at the same time as oxygen, if the inhibition of oxygen cannot be eliminated, the rate of hydrogen production is low and hydrogen cannot be produced continuously for a long time. Abroad, a lot of work has been done on the screening of aerobic strains. Scientists from the National Renewable Energy Laboratory (NREL) and the University of California successfully studied the two-step indirect photohydrolysis hydrogen production process of freshwater Chlamydomonas reversible hydrogenase, using the mutant Chlamydomonas reinhardtii CC- 124, achieve O 2 and H 2 The generation of hydrogena...

Examples

Embodiment 1

[0035] Such as figure 1Shown is a schematic diagram of the photobiological hydrogen production system, in which 3 is a photoreactor, made of glass materials, the gas volume change in the photoreactor 3 is collected and measured in the water tank 8 through the calibration gas sampling tube 7, and the consumption of the fuel cell The hydrogen current is automatically detected and recorded by the data acquisition and recording system. The subcardiac flat algae were cultured with Kangweifang nutrient salts. After culturing to the late logarithmic growth stage, they were collected by centrifugation until the concentration of the hydrogen-producing algae liquid was 8 million cells / mL. Using the two-step method of photolysis of water to produce hydrogen from flat algae, take a stirred photohydrogen reactor with a volume of 500mL, fill it with algae liquid, seal the bottle mouth, replace the air with nitrogen for 5 minutes, and place it in a 25°C incubator in the dark. Cultured to i...

Embodiment 2

[0040] The subcardiac flat algae are cultivated with Kangweifang nutrient salts. After culturing to the late logarithmic growth stage, they are centrifuged, collected by membrane filtration, or cultured at high density to a concentration of 6 million cells / mL in the hydrogen-producing algae liquid. The method of producing hydrogen by photolysis of water using two-step method of flat algae, the process is as follows figure 2 Show. Take a stirred-type photohydrogen production reactor with a volume of 500 mL, fill it with algae liquid, seal the bottle mouth, replace the air with nitrogen for 5 min, and place it in a 25°C incubator for dark cultivation to induce reversible hydrogenase. After 20 hours of dark induction to the algae solution, CCCP was added to a concentration of 15 μM before light, and the pH value was adjusted to 8.2 with sodium bicarbonate solution. The system stirs the algae liquid at a medium light intensity of 9000LX and a stirring speed of 150rpm, so that the...

Embodiment 3

[0047] The subcardiac flat algae are cultivated with Kangweifang nutrient salts. After culturing to the late logarithmic growth stage, they are centrifuged, collected by membrane filtration, or cultured at high density to a concentration of 6 million cells / mL in the hydrogen-producing algae liquid. The method of producing hydrogen by photolysis of water using two-step method of flat algae, the process is as follows figure 2 Show. Take a stirred-type photohydrogen production reactor with a volume of 500 mL, fill it with algae liquid, seal the bottle mouth, replace the air with nitrogen for 5 min, and place it in a 25°C incubator for dark cultivation to induce reversible hydrogenase. After 20 hours of dark induction, CCCP was added to the concentration of 7.5 μM before light, and the pH value was adjusted to 8.2 with sodium bicarbonate solution. The system stirs the algae liquid at a medium light intensity of 9000LX and a stirring speed of 150rpm, so that the algae liquid in th...

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