Method for producing biodiesel by utilizing immobilized lipase and adopting static emulsion method

Abstract

The invention relates to a method for producing biodiesel by utilizing immobilized lipase and adopting a static emulsion method. The method for producing biodiesel by utilizing immobilized lipase and adopting the static emulsion method comprises the following steps: (1) adding polydimethylsiloxane, lipase solution and a platinum catalyst into a reactor, carrying out vacuum magnetic stirring for 0.5-2 hours to obtain emulsion, then adding a dispersing agent into the emulsion to form composite emulsion, under the condition of mechanical stirring, solidifying for forming enzyme-loaded static emulsion silica gel microspheres, and filtering, so that immobilized lipase is obtained; and (2) adding vegetable fat and short chain alcohol into the reactor, then adding normal hexane with the volume 1-4 times that of the vegetable fat and immobilized lipase with the mass 20-30% that of the vegetable fat, and reacting at constant temperature of 20-600 DEG C, so that the biodiesel is obtained, wherein the lipase is candida antarctica lipase B, candida rogusa lipase or burkholderia cepacia lipase. The method for producing biodiesel by utilizing immobilized lipase and adopting the static emulsion method has the advantages of simple preparation technology, mild reaction conditions, no environmental pollution and high catalyst repeated utilization factor and is beneficial to industrial application of production of biodiesel.

Description

technical field [0001] The invention belongs to the field of biodiesel production by immobilized enzymes, and in particular provides a method for immobilizing lipase by a static emulsion method and applying the immobilized lipase to biodiesel production. Background technique [0002] Biodiesel is a clean and safe new fuel that can replace petrochemical diesel produced from renewable resources such as vegetable oil or animal oil. The main component is a mixture of a series of long-chain fatty acid ester compounds. Biodiesel has similar properties to petrochemical diesel, and has the characteristics of renewable, easy biodegradation, low sulfur content, low combustion pollutant emissions, and low greenhouse gas emissions. Therefore, the current production and research of biodiesel has received widespread attention. [0003] At present, the production of biodiesel mainly includes chemical and biological methods. The chemical method is to use animal and vegetable oils and short...

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Problems solved by technology

[0003] At present, the production of biodiesel mainly includes chemical and biological methods. The chemical method is to use animal and vegetable oils and short-chain alcohols such as methanol or ethanol to perform transesterification under acidic or basic catalysts and high temperatures (230-250 ° C). The chemical method has the following disadvantages: the process is complicated, excessive alcohol needs to be added, the subsequent process must have a corresponding alcohol recovery device, and the energy consumption is high; the esterification product is difficult to recover and the cost is high; the production process has waste lye discharge, polluting the environment

The above patents all use immobilization methods such as adsorption, cross-linking, and embedding to improve the shortcomings of free lipase, such as poor activity, susceptibility to external environment interference, and low operational stability.

[0005] Lipase is a typical interfacial enzyme. Its catalytic activity is easily "activated" at the oil-water interface. The amino acid cover in its three-dimensional structure will open and expose the hydrophobic active site to the oil phase to participate in the catalytic reaction. Therefore, lipase is The oil-water interface has stronger activity and selectivity than the main phase, but the immobilization methods used in the current patents are all single-phase system immobilized enzymes, which is not conducive to the interfacial activity of lipase. Therefore, a new type of lipase is studied. The method of immobilizing enzymes can not only maintain the natural catalytic activity of lipase for a long time, but also facilitate continuous large-scale production operations, which has certain practical significance for the industrial production of biodiesel

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