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A kind of method for enriching n-3 polyunsaturated fatty acid by enzymatic method

An unsaturated fatty acid, n-3 technology, applied in the field of oil deep processing, can solve the problem of insufficient precision and achieve the effect of low fatty acid content

Active Publication Date: 2022-08-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the problem that the accuracy of lipase selective hydrolysis for enriching n-3 polyunsaturated fatty acids in the above and / or existing methods for enriching polyunsaturated fatty acids by enzymatic methods is not high enough, the present invention provides a A method for enzymatically enriching polyunsaturated fatty acids. The present invention uses lipase derived from Candida cylindracea and lipase Candidaantarcticalipase A to catalyze the esterification of oils. Through the method of the present invention, saturated and monounsaturated fatty acids in oils can be The sum of fatty acid content is reduced to less than 20%

Method used

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  • A kind of method for enriching n-3 polyunsaturated fatty acid by enzymatic method
  • A kind of method for enriching n-3 polyunsaturated fatty acid by enzymatic method
  • A kind of method for enriching n-3 polyunsaturated fatty acid by enzymatic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Accurately weigh the fish oil (the fatty acid composition of the fish oil sample is as figure 1 shown, where n-3PUFA=34.3%, SFA%=36.3%, MUFA%=24.0%) 3.0 g, phosphate buffer solution 3 g (concentration 0.1 mol / L, pH 7) and AY "Amano" 400SD lipase 960U, added to the reaction kettle, put into the magnetic rotor, and sealed. The reaction kettle was placed on a magnetic stirrer, and the reaction kettle was connected to circulating water, and the water temperature was kept at a constant temperature of 37 °C for 10 h. After the reaction, remove the free fatty acid hydrolyzed with KOH-ethanol aqueous solution, wash with water for 3 times afterwards, get the upper strata clear oil phase, steam off the solvent and obtain the fish oil glyceride rich in n-3PUFAs (the fatty acid composition is as follows. figure 2 ).

[0044] Accurately weigh the obtained glyceride 10.0g (the product accumulated many times in the previous step), ethanol solution (alcohol-water mass ratio 1:4) 5g ...

Embodiment 2

[0046] Accurately weigh 10.0 g of fish oil, 5 g of ethanol solution (1:4 mass ratio of alcohol to water) and 4500 U of Candida antarcticalipase A lipase, add them to the reaction kettle, put them into a magnetic rotor, and seal them. The reaction kettle was placed on a magnetic stirrer, and the reaction kettle was connected to circulating water, and the water temperature was kept at a constant temperature of 37 °C for 10 h. After the reaction finishes, free fatty acid and ethyl ester in the product are removed by molecular distillation to obtain the fish oil glyceride rich in n-3PUFAs (fatty acid composition such as Figure 4 ).

[0047] Accurately weigh the obtained glyceride 3.0g, phosphate buffer solution 3g and AY "Amano" 400SD lipase 960U, add it to the reaction kettle, put it in a magnetic rotor, and seal it. The reaction kettle was placed on a magnetic stirrer, and the reaction kettle was connected to circulating water, and the water temperature was kept at a constant ...

Embodiment 3

[0049] Accurately weigh 3.0 g of fish oil, 3.0 g of phosphate buffer solution, AY "Amano" 400SD lipase 960U and Candidaantarctica lipase A lipase 1350U, add them to the reaction kettle, put them into a magnetic rotor, and seal them. The reaction kettle was placed on a magnetic stirrer, and the reaction kettle was connected to circulating water, and the water temperature was kept at a constant temperature of 37 °C for 10 h. After the reaction, the free fatty acid hydrolyzed by KOH-ethanol aqueous solution was removed, and then washed with water for three times, and the upper clear oil phase was taken, and the solvent was evaporated to obtain the fish oil glyceride rich in n-3PUFA. In the fatty acid composition of glyceride products, the content changes of n-3PUFA, SFA and MUFA are shown in Table 1.

[0050] The experimental conditions and results of Examples 4 to 19 are shown in Table 1 below, wherein, except for the marked conditions, the remaining operating parameters of Exam...

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Abstract

The invention discloses a method for enriching n-3 polyunsaturated fatty acids by an enzymatic method, and belongs to the field of oil and fat deep processing. The method of the invention comprises: taking oil, water (alcohol) solution and lipase to react in a reactor, wherein the lipase includes one or more lipases derived from Candida cylindracea and lipase Candida antarctica lipase A, And the order of adding lipase is not fixed. At present, the content of saturated and monounsaturated fatty acids in the glycerides obtained by enzymatic catalytic hydrolysis or alcoholysis enrichment PUFA reaction is still relatively high; The monounsaturated fatty acid has specificity, and further reduces the non-PUFA fatty acid content in the glyceride product, thereby obtaining a glyceride product with a higher content of PUFA.

Description

technical field [0001] The invention relates to a method for enriching n-3 polyunsaturated fatty acids by an enzymatic method, and belongs to the field of deep processing of oils and fats. Background technique [0002] Polyunsaturated fatty acids (PUFAs) have important biological significance to the human body, especially n-3PUFAs, which are widely reported internationally. Among them, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) can significantly prevent cardiovascular disease, assist inflammation repair, and promote the normal development of infant sensory organs, and are widely used in health food and medicines. [0003] The n-3PUFAs products on the market mainly have three forms: ethyl ester type, glyceride type and free fatty acid type. The content of n-3PUFAs in glyceride type is relatively low, about 30%, which cannot meet people's health care and medicinal use. need. The content of n-3PUFAs in the ethyl ester type and free fatty acid type can reach 9...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/6472
CPCC12P7/6472C12P7/6454
Inventor 陈烨王小三王熠璠
Owner JIANGNAN UNIV
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