Method for preparing 1,3-diglyceride through enzymolysis
A technology of diglyceride and enzymatic hydrolysis, which is applied in 1 field, can solve the problems of difficult large-scale production by direct esterification method, low purity of 1,3-DAG, complicated purification process, etc., so as to improve the 2-position selection tendency, The effect of cheap reaction raw materials and simple reaction process
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Embodiment 1
[0047]1. Mix 1000g soybean oil with 100g water, add 100g ethanol; homogenize at a high speed of 5000rpm for 20min to prepare an emulsion;
[0048] 2. Heat the emulsion to 70°C and keep it at a constant temperature, and adjust the pH of the emulsion to 7.0 with hydrochloric acid or sodium hydroxide;
[0049] 3. Add 20g of Candida antarctica lipase A;
[0050] 4. After 22 hours of reaction, the reaction ends;
[0051] 5. The reaction system was centrifuged, and the oil phase was molecularly distilled to separate monoglyceride and NEFA, diglyceride and triglyceride; the proportion of 1,3-diglyceride in the diglyceride phase was 83%.
Embodiment 2
[0053] 1. Mix 1000g soybean oil with 100g water, add 0.98g gluconic acid; homogenize at a speed of 5000rpm for 20min at high speed to prepare an emulsion;
[0054] 2. Heat the emulsion to 70°C and keep it at a constant temperature, and adjust the pH of the emulsion to 7.0 with sodium hydroxide or hydrochloric acid;
[0055] 3. Add 20g of Candida antarctica lipase A;
[0056] 4. After 19 hours of reaction, the reaction ends;
[0057] 5. The reaction system was centrifuged, and the oil phase was molecularly distilled to separate monoglyceride and NEFA, diglyceride and triglyceride; the proportion of 1,3-diglyceride in the diglyceride phase was 79%.
Embodiment 3
[0059] 1. Mix 1000g soybean oil with 100g water, add 100g ethanol and 0.98g gluconic acid; homogenize at a speed of 5000rpm for 20min at high speed to prepare an emulsion;
[0060] 2. Heat the emulsion to 70°C and keep it at a constant temperature, and adjust the pH of the emulsion to 7.0 with sodium hydroxide or hydrochloric acid;
[0061] 3. Add 20g of Candida antarctica lipase A;
[0062] 4. After 17 hours of reaction, the reaction ends;
[0063] 5. The reaction system was centrifuged, and the oil phase was molecularly distilled to separate monoglyceride and NEFA, diglyceride and triglyceride; the proportion of 1,3-diglyceride in the diglyceride phase was 85%.
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