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Method for preparing 1,3-diglyceride through enzymolysis

A technology of diglyceride and enzymatic hydrolysis, which is applied in 1 field, can solve the problems of difficult large-scale production by direct esterification method, low purity of 1,3-DAG, complicated purification process, etc., so as to improve the 2-position selection tendency, The effect of cheap reaction raw materials and simple reaction process

Inactive Publication Date: 2015-05-20
HANGZHOU DUOHAI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] However, the direct esterification method is difficult to achieve large-scale production, and there are mainly the following problems: first, the required raw materials are free fatty acids or glycerin, and the price is very high; second, the reaction is generally carried out in an organic solvent environment, and there is a problem of organic solvent residue
[0016] The glycerol solution method also has certain limitations, which are mainly reflected in the following aspects: first, the price of the reaction substrate glycerol is relatively high; second, the reaction speed is slow, and generally takes 10 hours or longer to complete the reaction; third, The purity of 1,3-DAG in the product is not high, and the purification process is complicated; the fourth is that the production rate of 1,3-DAG is not high, and only one molecule of 1,3-DAG can be produced from one molecule of triglyceride and one molecule of glycerol

Method used

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  • Method for preparing 1,3-diglyceride through enzymolysis
  • Method for preparing 1,3-diglyceride through enzymolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047]1. Mix 1000g soybean oil with 100g water, add 100g ethanol; homogenize at a high speed of 5000rpm for 20min to prepare an emulsion;

[0048] 2. Heat the emulsion to 70°C and keep it at a constant temperature, and adjust the pH of the emulsion to 7.0 with hydrochloric acid or sodium hydroxide;

[0049] 3. Add 20g of Candida antarctica lipase A;

[0050] 4. After 22 hours of reaction, the reaction ends;

[0051] 5. The reaction system was centrifuged, and the oil phase was molecularly distilled to separate monoglyceride and NEFA, diglyceride and triglyceride; the proportion of 1,3-diglyceride in the diglyceride phase was 83%.

Embodiment 2

[0053] 1. Mix 1000g soybean oil with 100g water, add 0.98g gluconic acid; homogenize at a speed of 5000rpm for 20min at high speed to prepare an emulsion;

[0054] 2. Heat the emulsion to 70°C and keep it at a constant temperature, and adjust the pH of the emulsion to 7.0 with sodium hydroxide or hydrochloric acid;

[0055] 3. Add 20g of Candida antarctica lipase A;

[0056] 4. After 19 hours of reaction, the reaction ends;

[0057] 5. The reaction system was centrifuged, and the oil phase was molecularly distilled to separate monoglyceride and NEFA, diglyceride and triglyceride; the proportion of 1,3-diglyceride in the diglyceride phase was 79%.

Embodiment 3

[0059] 1. Mix 1000g soybean oil with 100g water, add 100g ethanol and 0.98g gluconic acid; homogenize at a speed of 5000rpm for 20min at high speed to prepare an emulsion;

[0060] 2. Heat the emulsion to 70°C and keep it at a constant temperature, and adjust the pH of the emulsion to 7.0 with sodium hydroxide or hydrochloric acid;

[0061] 3. Add 20g of Candida antarctica lipase A;

[0062] 4. After 17 hours of reaction, the reaction ends;

[0063] 5. The reaction system was centrifuged, and the oil phase was molecularly distilled to separate monoglyceride and NEFA, diglyceride and triglyceride; the proportion of 1,3-diglyceride in the diglyceride phase was 85%.

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Abstract

The invention relates to a method for preparing 1,3-diglyceride through enzymolysis, and belongs to the technical field of 1,3-diglyceride. The method comprises the step of adding ethanol or / and chelating agent in TAG, water and Candida antarctica lipase A to form a hydrolysis system. According to the method, the chelating agent is added in the TAG hydrolysis system to remove metal ion (mainly from the TAG), thereby reducing the inhibiting effect of the metal ion to the enzyme activity and improving the hydrolysis rate; after ethanol and the chelating agent are simultaneously added, not only is the selection tendency of the Candida antarctica lipase A to 2 position of the TAG improved and the reaction time shortened, but also the improvement range of the hydrolysis speed is further increased; therefore, ethanol plays the effect of promoting the chelating agent to improve the hydrolysis speed.

Description

technical field [0001] The invention relates to a method for preparing 1,3-diglyceride by enzymatic hydrolysis, and belongs to the technical field of preparation of 1,3-diglyceride. Background technique [0002] Fat is an important part of human diet, and it undertakes functions such as providing energy for the body and improving food flavor during food processing. With the development of the economy and the improvement of people's living standards, the oil intake of Chinese residents is increasing day by day. Many studies have shown that excessive intake of oil will increase the risk of metabolic syndrome such as atherosclerosis, type 2 diabetes, hyperlipidemia, and hypertension, which seriously threatens the health of the body. [0003] 1,3-Diglyceride (DAG) is the main component of natural oils, and there is no significant difference between its color, energy density, odor, stability and bioavailability in the body and traditional triglyceride (TAG). difference. Many ...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCC12P7/6445
Inventor 李霞徐同成李铎冀延春史俊蒋海军
Owner HANGZHOU DUOHAI TECH
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