94 results about "Candida candida" patented technology

The invention discloses diglyceride prepared by an enzymic method as well as a preparation method and application thereof, and belongs to the technical field of deep processing of grease. The method comprises the following steps: adding fatty acid into a choline chloride-glycerol-urea ternary eutectic solvent, uniformly mixing, and then adding candida antarctica lipase B for esterification reaction to obtain a reaction mixture; separating the reaction mixture, and recovering an upper-layer oil phase; and separating and purifying the upper-layer oil phase to obtain the diglyceride. According to the method, the reaction rate is high, the selectivity of the diglyceride in the esterification process is high, the yield of the diglyceride can reach 65% or above by reacting for 1 h, the selectivity of the diglyceride is 75% or above, and the purity of the separated and purified diglyceride is 90% or above; in addition, the method is simple to operate, and the reaction product and the eutectic solvent can be separated through simple centrifugation after the reaction is finished; in addition, the monoglyceride content in the product is low, and the subsequent separation and purification difficulty is greatly reduced.

The invention discloses candida utilis engineering bacteria and a method for producing single-cell protein by fermenting corn thin pulp, and belongs to the technical field of biology. According to the candida utilis engineering bacteria disclosed by the invention, the candida utilis is subjected to gene modification, a chitin synthase gene in the candida utilis is knocked out, and an exogenous keratinase gene is integrated, so that the candida utilis engineering bacteria can utilize corn thin pulp as a substrate for fermentation. In the fermentation process, carbon and nitrogen sources in the thin corn pulp can be effectively utilized for growth, macromolecular protein in the thin corn pulp can be hydrolyzed and converted into single-cell protein, the content of the single-cell protein in final fermentation liquor is increased, the protein content of the final fermentation liquor can reach 60% or above, and the proportion of the single-cell protein can reach 30% or above; the corn thin pulp can be efficiently utilized, and meanwhile, single-cell protein in the fermentation liquor can be used for adding animal feed so as to meet the requirement for animal protein feed in the market.

The application belongs to the field of microorganism and fermentation engineering, and provides a Candida magnoliae with high yield of 3-methylthiopropanol, and the preservation number is CGMCC NO. 23668. The Candida magnoliae can be used for producing 3-methylthiopropanol, the yield can reach 3.16 g / L, and the ethanol tolerance is high, and can be applied in the wine making, condiment and food additive industries.

The invention relates to the technical field of microbial fermentation, and provides a method for producing single-cell protein, which comprises the following steps: inoculating a crop straw matrix with a first microbial seed for first fermentation; the first microbial seed comprises trametes versicolor; sterilizing a fermentation product obtained by the first fermentation; inoculating second microbial seeds into the sterilized fermentation product for second fermentation; the second microbial seed comprises yarrowia lipolytica / candida utilis. According to the scheme, the combination of the selected first microbial seed solution and the second microbial seed solution has a remarkable synergistic effect, so that the crude protein content, the lignin removal rate, the cellulose retention rate and the yeast biomass are improved, and the hemicellulose loss rate is reduced.

1. Name of the designed product: packing bag (NFP50 feed raw material Candida utilis protein). 2. Use of the designed product: used for packing feed. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: front view. 5. The designed product is a thin product, and the bottom view, top view, left view and right view are omitted.

The invention belongs to the technical field of directed evolution modification and biological catalysis application of enzymes, and relates to a Candida antarctica lipase B mutant protein and application thereof, the sequence of the mutant protein comprises one or more of the mutation of the 195 S and F, the mutation of the 195 S and Y and the mutation of the 283 A and Y from the N terminal to the C terminal of the amino acid sequence shown in SEQ ID NO.1 of the original CALB; on the basis of a wild type lipase gene sequence, through rational design and site-specific mutagenesis, mutants S195F, S195Y, A283Y, S195F-A283Y and S195Y-A283Y, of which the Tm value and the optimal reaction temperature are obviously improved and the catalytic activity is improved by 1-2 times, are finally obtained; when the mutant is used for catalyzing synthesis of docosahexaenoic acid (DHA) glyceride, higher catalytic efficiency is shown, and the yield of the DHA glyceride is effectively improved.

The invention discloses a uricase mutant. The uricase sequence from aspergillus flavus and the uricase sequence from candida are taken as templates, multiple rounds of random mutation are carried out, sequencing verifies that the key amino acid site of the catalytic center is mutated, and the catalytic activity is enhanced. In-vivo and in-vitro uric acid reducing activities of the two uricase mutants are further verified, and the two uricase mutants can significantly reduce the content of uric acid and are expected to become new uricase drugs for treating hyperuricemia.

The invention provides a Candida viscera CATH2303 (Candida viscera CATH2303) and an application of the Candida viscera CATH2303. The Candida viscera CATH2303 The candida vesii provided by the invention has reduced fatty alcohol dehydrogenase activity or does not have fatty alcohol dehydrogenase activity, and can efficiently utilize fatty acid or derivatives thereof as a substrate to produce omega-hydroxy fatty acid, and the yield is obviously improved. The candida vesii and the method for producing the omega-hydroxy fatty acid by using the candida vesii, provided by the invention, have the potential of industrial large-scale production.

The invention relates to the technical field of cosmetics, and particularly discloses an anti-hair-loss composition as well as a preparation method and application thereof. The invention relates to a preparation method of an anti-hair loss composition, which comprises the following steps: cleaning and drying a fermentation substrate, crushing into slurry, sterilizing, inoculating lactobacillus plantarum, candida tropicalis, schizosaccharomyces cerevisiae and monascus purpureus strains at a proper temperature, carrying out sealed fermentation, and after fermentation is completed, carrying out centrifugal separation on the fermentation liquor to obtain the anti-hair loss composition. Taking supernate, sterilizing, and performing vacuum freeze drying to obtain the anti-alopecia composition. According to the application of the anti-hair-loss composition, the anti-hair-loss composition is added into a hair product. The anti-hair loss composition disclosed by the invention is natural in source, and can promote absorption of skin to active substances, so that the anti-hair loss composition has the effects of rapidly inhibiting scalp oil and reducing hair loss.

This invention discloses an overexpression Met15 This invention relates to a genetically engineered strain of *Candida utilis*, its construction method, and its applications, belonging to the field of bioengineering technology. The starting strain of the genetically engineered strain described in this invention is *Candida utilis*. C. utilis CCTCC NO: M 209298, strain overexpressing O-acetylhomoserine thioylase. Met15 Gene. This invention utilizes gene recombination technology to overexpress the O-acetylhomoserine sulfhydrylase encoding gene in *Candida utilis* (…). Met15 This can increase the content of L-methionine and L-cysteine ​​in yeast cells, increase the supply of sulfur-containing amino acid precursors, and ultimately increase the yield of SAM and GSH co-production in yeast cells.

The invention discloses a compound microorganism feed additive for promoting the growth of livestock and poultry. The compound microorganism feed additive comprises microorganism strains, natural plant components and auxiliary materials, the microbial strains comprise 8 to 12 parts of candida utilis, 5 to 9 parts of bacillus licheniformis, 2 to 4 parts of bacillus subtilis, 8 to 12 parts of aspergillus niger, 8 to 12 parts of lactobacillus delbrueckii, 8 to 12 parts of lactobacillus acidophilus, 8 to 12 parts of bifidobacterium and 3 to 7 parts of lactobacillus salivarius; the natural plant components comprise 0.5 to 2 parts of moringa seed powder; the auxiliary materials comprise 20 to 30 parts of cane sugar, 10 to 15 parts of fish meal, 2 to 3 parts of grease, 10 to 20 parts of soybean meal, 5 to 10 parts of pupa powder, 10 to 15 parts of zinc lactate, 1 to 2 parts of calcium gluconate, 3 to 5 parts of calcium carbonate and 2 to 4 parts of paeonol. Microbial strains in the compound microbial feed additive can generate various growth regulatory factors such as vitamins, amino acids and organic acids, so that the growth and development of livestock and poultry are stimulated, and the growth speed is increased.

The application belongs to the technical field of protein expression, and relates to a gene expression cassette for improving the yield of methionine of Candida utilis, an expression vector, genetically engineered bacteria, a method and application. The application provides application of a GAP-P mutant promoter GP6 and a homologous signal peptide SP8 in improving the yield of methionine of Candida utilis; the nucleotide sequence of the GP6 is shown in SEQ ID NO. 1, and the nucleotide sequence of the SP8 is shown in SEQ ID NO. 2. The application realizes the improvement of the yield of methionine through the improvement of the strength of the promoter and the screening of the homologous signal peptide.

The present application relates to the field of genetic engineering and bioengineering technology, in particular to a Candida antarctica lipase B mutant and application thereof. The Candida antarctica lipase B mutant provided by the present application is obtained by joint mutation of the 143th and 284th positions in the amino acid sequence shown in the wild type lipase B. The Candida antarctica lipase B mutant constructed by the present application has 12.5 times higher enzyme activity than the wild type enzyme, and the protein expression amount is as high as 1.8-1.9 g / L under the scale of 5 L fermenter, which can significantly improve the yield and reduce the production cost. In addition, the Candida antarctica lipase B mutant can be used for synthesis of high value-added, non-natural substrates such as vitamin C fatty acid ester and vitamin A oleate, which lays a foundation for the application of lipase in the field of fine chemical industry.

The invention discloses a preparation process of a multi-stage fermentation biological feed, and belongs to the technical field of feed biology. In order to solve the problems that the existing fermented feed is poor in strain synergism, insufficient in enzymolysis, low in probiotic colonization amount and the like, the method adopts a staged and time sequence inoculation strategy, and four stages of aerobic enzyme production degradation, aerobic fermentation and enzymolysis, facultative anaerobic fermentation and anaerobic probiotic colonization are sequentially carried out; and the inoculation sequence and proportion of trichoderma reesei, aspergillus niger, aspergillus oryzae, bacillus subtilis, candida utilis and lactobacillus plantarum are accurately controlled. By constructing an efficient flora metabolic chain, the quality of the feed is remarkably improved. The crude protein content of the prepared feed can reach 37.8% or above, and the viable count of lactobacillus plantarum reaches 3.1 * 10 < 10 > CFU / g. The prepared feed product has the advantages of high nutritional value, strong probiotic function and long shelf life, and the process is controllable and suitable for industrial production.

The invention discloses a method for rapidly detecting dichlorvos on site based on scattering turbidity, and belongs to the technical field of food and agricultural product safety detection. The method comprises the following steps: incubating dichlorvos with different concentrations with candida antarctica lipase, a phosphate buffer solution and L-ascorbyl palmitate, centrifugally incubating to obtain a reaction solution, taking a supernatant to react with a manganese dioxide nanosheet, measuring the turbidity of the reaction solution through a portable turbidimeter, and establishing a linear equation of the concentration and the turbidity of the dichlorvos; by measuring the turbidity of an unknown sample, the concentration of the dichlorvos can be calculated according to a linear equation. According to the method, invisible dichlorvos concentration signals are converted into measurable turbidity signals by utilizing the synergistic effect of enzyme inhibition reaction and turbidity change induction of the nano material, so that rapid and sensitive detection of dichlorvos is realized. The method is easy and convenient to operate, low in cost, high in detection speed, free of large instruments and especially suitable for on-site rapid screening of agricultural products.

The present application relates to a kind of containing fatty acyl-coa dehydrogenase engineering bacteria and its application method, belong to microbial fermentation technical field.The engineering bacteria containing fatty acyl-coa dehydrogenase gene in the present application include fatty acyl-coa dehydrogenase gene and fatty acyl-coa thioesterase gene, the fatty acyl-coa dehydrogenase gene is derived from Candida tropicalis or Yarrowia lipolytica, its nucleotide sequence is SEQ ID NO.1 or SEQ ID NO.2;The nucleotide sequence of the fatty acyl-coa thioesterase gene is SEQ ID NO.3.Fatty acyl-coa dehydrogenase from Candida tropicalis and Yarrowia lipolytica, combined with existing fatty acyl-coa thioesterase, jointly convert 10-hydroxy decanoic acid, significantly improve the conversion rate of 10-hydroxy-2-decenoic acid, so that the industrialization production of 10-hydroxy-2-decenoic acid becomes possible.

The invention discloses a targeted compound probiotic preparation for producing SOD (superoxide dismutase) eggs, which is prepared from the following active ingredients in percentage by mass: 5 to 15 percent of bacillus licheniformis, 3 to 10 percent of pseudomonas strain NJ-62, 15 to 30 percent of saccharomyces boulardii, 10 to 20 percent of lactobacillus plantarum, 8 to 15 percent of bacillus subtilis, 10 to 20 percent of candida utilis and 5 to 15 percent of lactobacillus acidophilus. The invention relates to the technical field of healthy breeding of livestock and poultry and production of functional food, in particular to integrated production of'safety 'and'function', specifically, targeted biological detoxification and nutrition metabolism induction technologies are deeply integrated for the first time, and complex probiotic groups remove antibiotics, heavy metals and other endogenous and exogenous pollutants in advance, so that a'clean 'physiological environment is created for laying hens; on the basis, efficient synthesis of SOD is specifically induced, and the problem of potential safety hazards of the functional eggs is fundamentally solved.

The application discloses a Candida antarctica lipase B mutant and a preparation method thereof, and belongs to the technical field of biology. Eleven mutants with improved enzyme activity are obtained, including six unit point mutants and five combined mutants; compared with a wild type, the enzyme activity of the mutants Q11L, F71N, F118Y, V149T, L219N, T244D, S31T / F71N, F71N / F118Y, F71N / L219N, S31T / F71N / F118Y and F71N / F118Y / L219N is improved, and is respectively increased by 18.45% to 240.88%; the CALB mutant with high enzyme activity is obtained, and has great industrial application potential and economic value.

The invention relates to ceramide liposome as well as a preparation method and application thereof. The ceramide liposome is prepared from the following raw materials: water, an alcohol solvent, phospholipid, cholesterol, a co-emulsifier and an active component, the active components comprise ceramide and a candida albicans / glucose / flat nuclear wood oil fermentation product. The invention develops a liposome product simultaneously loading ceramide and candida albicans / glucose / flat nuclear wood oil fermentation products, the two active components are mutually matched and have synergistic interaction in the aspect of skin barrier repair, the transdermal permeation effect of the active components can be improved by virtue of a liposome form, and the bioavailability of the liposome product is improved. Compared with unfermented flat nuclear wood oil, the candida albicans / glucose / flat nuclear wood oil fermentation product is selected, so that the hydrophilicity and stability of the liposome can be improved, the encapsulation performance of the liposome on ceramide and the transdermal permeation effect of the ceramide can be improved, and finally the skin barrier repairing effect of the liposome product is improved.

The invention relates to an amino acid-containing water-soluble fertilizer and a preparation method thereof, and the amino acid-containing water-soluble fertilizer is prepared by the following steps: (1) crushing a waste protein raw material, adding water and a compound enzyme, mixing, performing enzymolysis, and performing enzyme deactivation to obtain an enzymolysis product; (2) inoculating Candida utilis and Pseudomonas delbrueckii into the enzymatic hydrolysate for fermentation, and filtering to obtain a fermentation filtrate; and (3) adding a plant growth regulator, urea, monopotassium phosphate, potassium sulfate, ferrous sulfate and zinc sulfate into the fermentation filtrate to prepare the amino acid-containing water-soluble fertilizer. According to the preparation method disclosed by the invention, the compound enzyme consisting of protease is firstly adopted for enzymolysis, and then the candida utilis and the pseudomonas delbrueckii are adopted for fermentation, so that the content of free amino acids in the fermentation filtrate can be increased, and the use effect of the amino acid-containing water-soluble fertilizer is further improved.

The application provides a strain of Candida zeylanoides, and applications of the strain in inhibiting Botrytis cinerea and in wine brewing, the Candida zeylanoides is numbered SIVE8902, has been preserved in the China Center for Type Culture Collection on July 22, 2024, the preservation number is CCTCC NO:M 20241637, and the Latin name is Starmerella bacillaris The strain has a strong inhibiting effect on Botrytis cinerea in grape fresh fruits and vineyards, can reduce the growth and reproduction of Botrytis cinerea in the fruits, and meanwhile, compared with single fermentation of Saccharomyces cerevisiae, the sequential fermentation of the Candida zeylanoides and Saccharomyces cerevisiae adds ethylguaiacol and 4-ethyl-2-methoxyphenol, adds spice and honey flavors to the wine, greatly improves the total amount of volatile phenolic compounds in the wine, and improves the wine quality.

The invention relates to the technical field of plant cultivation, in particular to an edible mushroom cultivation material and a preparation method and application thereof. The invention provides an edible mushroom cultivation material. The edible mushroom cultivation material comprises 10-25 parts of corncob, 10-20 parts of rape straw, 5-8 parts of soybean meal, 1-3 parts of leonurine, 1-2 parts of naringenin, 1-2 parts of sugar and 1-3 parts of a complex microbial inoculant, the complex microbial inoculants comprise candida utilis, lactobacillus plantarum, trichoderma harzianum, pseudomonas fluorescens and pediococcus pentosaceus. According to the edible mushroom cultivation material provided by the invention, each component has different effects and can generate a synergistic effect at the same time, so that the yield of edible mushrooms is increased to the maximum extent, the contamination rate of infectious microbes can be reduced, and a new direction is provided for edible mushroom cultivation.

The invention relates to a mutated Candida antarctica lipase B. The invention also relates to a gene encoding the enzyme, and a vector and a host cell comprising the gene. In addition, the invention also relates to a preparation method and application of the enzyme.

The application discloses a kind of protein feed using potato residue solid fermentation and a preparation method thereof.Mainly with potato residue as main raw material, aspergillus niger, Candida tropicalis, Candida lipolytica and Saccharomyces cerevisiae are used as collaborative fermentation strains to carry out aerobic-anaerobic fermentation, and through single factor test and orthogonal test, the production process of solid fermentation bacterial protein feed using potato residue as raw material is studied.The bacterial protein feed process is produced by adopting composite strain solid fermentation, which can significantly increase the crude protein content and true protein content in fermentation product, improve the nutritional value, improve animal growth performance, and increase the shelf life and stability of protein feed, and also provides a new mode for non-grain type feed resources, especially potato residue waste resource utilization, effectively alleviates the plight of conventional feed resource shortage.

The present application relates to a kind of veterinary immunity-enhancing antiviral powder and its preparation method, the veterinary immunity-enhancing antiviral powder includes traditional Chinese medicine fermentation powder 60-70%, phoenix tree seed oil 20-30% and dioscorea opuloides mucopolysaccharide 10-20% according to mass percentage;The traditional Chinese medicine fermentation powder is first obtained fermentation product using bacillus velezensis, Candida utilis fermentation scutellaria baicalensis, astragalus, pomegranate peel, hawthorn and polygonatum sibiricum, then is formed by drying, ultrafine grinding to fermentation product.This application can improve the quality concentration of piglet serum immunoglobulin, so that piglet immunity, antiviral capacity is improved, in addition, it has good therapeutic effect on piglet viral gastroenteritis.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a Candida citrina strain and an application of the Candida citrina strain. In order to develop more non-saccharomyces cerevisiae resources applied to fruit wine fermentation, the invention provides a new Candida citriodora, the preservation number of the Candida citriodora is CGMCC No.34757, and the Candida citriodora is applied to fruit wine fermentation. Compared with commercial non-saccharomyces cerevisiae NS-D, the Candida citriodora XP0136 provided by the invention shows good fermentation performance in a high tannin environment, has good adaptability to various stress environments such as high sugar, high ethanol, high SO2 and high acidity conditions, simultaneously produces H2S and acetic acid at low yield, and is beneficial to reducing generation of bad flavors. Besides, in the application of aronia melanocarpa fruit wine aroma enhancement brewing, the strain can enhance the typical aroma of brewed fruit wine, and a new strain source is provided for yeast suitable for brewing high-tannin fruit wine.

The present application relates to the technical field of oil and fat chemistry, and particularly relates to a preparation method and application of a diglyceride oil with the function of improving cardiovascular and cerebrovascular health. The preparation method of the diglyceride oil comprises the following steps: (1) mixing oil and water, adding a first hydrolytic enzyme to perform first hydrolysis, and then adding a second hydrolytic enzyme to perform second hydrolysis, to obtain a hydrolysis product; (2) adding the hydrolysis product to glycerol and a lipase to perform reaction, and then distilling, to obtain the diglyceride oil; in step (1), the first hydrolytic enzyme is Lipozyme RM IM, and the second hydrolytic enzyme is Candida antarctica lipase B; in step (2), the lipase is a mixture of lipase Lipase G50 and Rhizomucor niveus lipase, and the mass ratio of the two is 1:1-3. The diglyceride oil prepared by the method has the function of improving cardiovascular and cerebrovascular health.

The invention relates to the technical field of microbial fermentation, and discloses a pasture leavening agent for improving crude protein, reducing crude fiber and improving digestibility, which comprises three strains, namely aspergillus niger, candida utilis and lactobacillus pentosus. The invention relates to a self-induced peptide premix, which is composed of a covalent complex, a lignin-hemicellulose-L-arabinose, a phosphorylated urea-glycine chelate, a synthetic self-induced peptide premix and a carrier. According to the method, a specific initial carbon source is provided for aspergillus niger through LHLAC to start fiber degradation, synchronous slow release of a nitrogen source is achieved through PUGC, and protein anabolism of candida utilis is activated through sAIP. According to the scheme, a time sequence synergistic mechanism of fiber degradation, protein synthesis and acidification preservation is constructed, the problem of low efficiency caused by mismatching of all metabolic processes in the prior art is solved, and effective degradation of crude fibers of forage grass, great increase of crude protein content and remarkable improvement of final digestibility are realized.

The present application relates to a kind of Candida tropicalis engineering bacteria and its preparation method and the method for treating sewage, belong to genetic engineering technical field.Candida tropicalis engineering bacteria with Candida tropicalis CBW-2 ΔURA3 (preservation number: CGMCC NO.31647) as chassis strain, knock out ACS gene and transfer into promoter heterologous expression alpha-amylase gene;The promoter is endogenous promoter.Candida tropicalis is a kind of unconventional yeast with industrial significance, with the characteristics of fast cell growth, strong environmental tolerance, has the potential of sewage treatment.The present application significantly reduces the activity of acetyl-coenzyme A synthetase by knocking out ACS gene, increases the acetic acid accumulation of strain.And by high-efficiency expression alpha-amylase, starch component can be efficiently converted into acetic acid, can provide acetic acid as carbon source for heterotrophic nitrification-aerobic denitrification strain, without adding exogenous carbon source.

The invention relates to the technical field of cosmetics, and particularly discloses a plant seed oil composition as well as a preparation method and application thereof. The plant seed oil composition is prepared from a component A, a component B and a component C. The component A is prepared from, by mass, 32-35 parts of isononyl isononanoate and 0.01-0.05 part of ormosia henryi bark extract, and the component C is prepared from, by mass, 1.8-2.2 parts of hydroxypinacolone retinoate, 1-1.5 parts of candida albicans fermented oil, 0.8-1.2 parts of prunus amygdalus dunn seed oil, 0.4-0.8 part of millettia indica extract and 1.5-2.5 parts of isosorbide dimethyl ether. And the component B comprises 55-65 parts of an oil phase solvent. The plant seed oil composition can be used for essence oil, and has the advantage of improving the permeation and absorption effects of the essence oil in cuticle.