15 results about "Candida candida" patented technology

The application belongs to the field of microorganism and fermentation engineering, and provides a Candida magnoliae with high yield of 3-methylthiopropanol, and the preservation number is CGMCC NO. 23668. The Candida magnoliae can be used for producing 3-methylthiopropanol, the yield can reach 3.16 g / L, and the ethanol tolerance is high, and can be applied in the wine making, condiment and food additive industries.

The present application relates to the field of genetic engineering and bioengineering technology, in particular to a Candida antarctica lipase B mutant and application thereof. The Candida antarctica lipase B mutant provided by the present application is obtained by joint mutation of the 143th and 284th positions in the amino acid sequence shown in the wild type lipase B. The Candida antarctica lipase B mutant constructed by the present application has 12.5 times higher enzyme activity than the wild type enzyme, and the protein expression amount is as high as 1.8-1.9 g / L under the scale of 5 L fermenter, which can significantly improve the yield and reduce the production cost. In addition, the Candida antarctica lipase B mutant can be used for synthesis of high value-added, non-natural substrates such as vitamin C fatty acid ester and vitamin A oleate, which lays a foundation for the application of lipase in the field of fine chemical industry.

The application provides a strain of Candida zeylanoides, and applications of the strain in inhibiting Botrytis cinerea and in wine brewing, the Candida zeylanoides is numbered SIVE8902, has been preserved in the China Center for Type Culture Collection on July 22, 2024, the preservation number is CCTCC NO:M 20241637, and the Latin name is Starmerella bacillaris The strain has a strong inhibiting effect on Botrytis cinerea in grape fresh fruits and vineyards, can reduce the growth and reproduction of Botrytis cinerea in the fruits, and meanwhile, compared with single fermentation of Saccharomyces cerevisiae, the sequential fermentation of the Candida zeylanoides and Saccharomyces cerevisiae adds ethylguaiacol and 4-ethyl-2-methoxyphenol, adds spice and honey flavors to the wine, greatly improves the total amount of volatile phenolic compounds in the wine, and improves the wine quality.

The present application relates to a kind of veterinary immunity-enhancing antiviral powder and its preparation method, the veterinary immunity-enhancing antiviral powder includes traditional Chinese medicine fermentation powder 60-70%, phoenix tree seed oil 20-30% and dioscorea opuloides mucopolysaccharide 10-20% according to mass percentage;The traditional Chinese medicine fermentation powder is first obtained fermentation product using bacillus velezensis, Candida utilis fermentation scutellaria baicalensis, astragalus, pomegranate peel, hawthorn and polygonatum sibiricum, then is formed by drying, ultrafine grinding to fermentation product.This application can improve the quality concentration of piglet serum immunoglobulin, so that piglet immunity, antiviral capacity is improved, in addition, it has good therapeutic effect on piglet viral gastroenteritis.

The present application relates to the technical field of oil and fat chemistry, and particularly relates to a preparation method and application of a diglyceride oil with the function of improving cardiovascular and cerebrovascular health. The preparation method of the diglyceride oil comprises the following steps: (1) mixing oil and water, adding a first hydrolytic enzyme to perform first hydrolysis, and then adding a second hydrolytic enzyme to perform second hydrolysis, to obtain a hydrolysis product; (2) adding the hydrolysis product to glycerol and a lipase to perform reaction, and then distilling, to obtain the diglyceride oil; in step (1), the first hydrolytic enzyme is Lipozyme RM IM, and the second hydrolytic enzyme is Candida antarctica lipase B; in step (2), the lipase is a mixture of lipase Lipase G50 and Rhizomucor niveus lipase, and the mass ratio of the two is 1:1-3. The diglyceride oil prepared by the method has the function of improving cardiovascular and cerebrovascular health.

The application discloses a method for preparing yellow fermentation oil by fermenting traditional Chinese medicinal materials, the yellow fermentation oil and application thereof. The method comprises the following steps: adding vegetable oil and traditional Chinese medicinal material powder into a fermentation medium, inoculating a Candida bombicola seed liquid, and obtaining a fermentation liquid by fermentation; inactivating the fermentation liquid by heating, cooling, centrifugal treatment, collecting an oil phase, and obtaining a fermentation oil crude product; performing adsorption treatment on the fermentation oil crude product, and filtering to obtain the yellow fermentation oil. The traditional Chinese medicinal material powder comprises turmeric root powder and radix scrophulariae root powder with a mass ratio of (1-3):1. The total mass of the traditional Chinese medicinal material powder is 1.6% to 3.2% of the mass of the fermentation medium. The mass of the vegetable oil is 40% to 60% of the mass of the fermentation medium. The inoculation viable cell count of the Candida bombicola is 4.5x10 5 cfu / mL to 5.5x10 6 cfu / mL. The yellow fermentation oil prepared in the application has significantly improved color stability under the synergistic fermentation of traditional Chinese medicinal materials, and has the effects of firming, anti-wrinkling and repairing.

The present application relates to the technical field of fermentation detection, and particularly relates to a camellia oil fermentation detection method of bear-born candida, which comprises the following steps: preparing camellia oil emulsion; mixing the camellia oil emulsion with bear-born candida seed liquid, and feeding gas to obtain fermentation liquid; detecting the stirring shaft torque of the fermentation tank; adjusting the feeding rate of the camellia oil emulsion; detecting the attenuation rate of the volume oxygen transfer coefficient of the fermentation liquid after the feeding rate is adjusted; adjusting the aeration mode of the fermentation liquid, including adjusting the gas-liquid contact time or carrying out pulse aeration; continuously monitoring the oil-water liquid level potential of the fermentation liquid after the aeration mode is adjusted; determining whether the fermentation liquid reaches the fermentation end point according to the change curve of the oil-water liquid level potential; if the fermentation liquid reaches the fermentation end point, determining the stirring speed of the stirring shaft; and obtaining the bear-born candida after fermentation. The present application realizes the improvement of the accuracy of fermentation process control.

This invention belongs to the field of microbial technology, specifically a high-protein-producing *Candida utilis* strain and its applications. *Candida utilis* (… Cyberlindnera jadinii )24-3, deposited on November 27, 2025 at the Guangdong Provincial Microbial Culture Collection Center, accession number GDMCC No:67370. This high-protein Candida utilis produces single-cell protein. This invention relates to the production of single-cell protein by Candida utilis. C. jadinii 2.615 strain was mutagenized to obtain the mutant strain Candida utilis 24-3. The mutant strain was then used to rapidly and cost-effectively produce single-cell protein by using bacterial bran hydrolysate or the product of industrial waste gas treatment as substrates, thereby reducing the treatment costs of the two types of waste.

The application belongs to the field of genetic engineering and biological fermentation technology, and relates to a Bombus candidus Candida albicans engineering bacterium and a construction method and application thereof. An ADH1 gene of the Bombus candidus Candida albitis engineering bacterium is knocked out. The ability of the engineering bacterium to utilize linoleic acid is enhanced. By using the engineering bacterium, the yield can be improved, the fermentation time can be shortened, and the production cost can be reduced in the process of fermenting and synthesizing sophorolipids, thereby bringing actual value to industrialized large-scale production and application.

The application discloses a preparation method of a key intermediate (R)-2-(7-hydroxy-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl) acetic acid of itromifene, which comprises the following steps: taking (E)-2-(2-morpholino cyclopent-2-en-1-ylidene) ethyl acetate and (4-methoxyphenyl) hydrazine hydrochloride as starting materials, carrying out condensation and cyclization, hydrogenation and demethylation to obtain an intermediate 2-(7-hydroxy-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl) ethyl acetate, and then hydrolyzing the intermediate under the action of Candida antarctica lipase B to obtain the (R)-2-(7-hydroxy-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl) acetic acid. The intermediate can be used for preparing itromifene arginine salt.

The application discloses a microorganism, a microbial agent and application thereof. The microorganism is Candida sp., which is preserved in the China General Microbiological Culture Collection Center on May 27, 2024, and the preservation number is CGMCC No. 30793. The new strain of Candida sp. screened in the application has the advantages of fast growth speed, strong salt and acid tolerance, and the ability to inhibit the growth of pathogenic bacteria and to metabolize nitrate. The production method of food or feed developed by using the new strain and the microbial agent has the advantages of high salt tolerance, high fermentation efficiency, short production cycle, and the fermented food prepared by using the new strain has the advantages of rich yeast content, low nitrate content, nutrition, health, and unique flavor.

This invention provides a composition with hepatoprotective and intestinal-protective effects, its preparation method, and its application, belonging to the field of aquaculture technology. The composition with hepatoprotective and intestinal-protective effects provided by this invention is made from the following raw materials: Gynostemma pentaphyllum, Hippophae rhamnoides, Euryale ferox, pumpkin seeds, Polygonatum odoratum, Scutellaria baicalensis, Houttuynia cordata, and a compound microbial agent, wherein the compound microbial agent is composed of Candida utilis, Bacillus natto, and Saccharomyces cerevisiae. The composition provided by this invention is entirely non-toxic and contains no prohibited ingredients. Its medicinal properties are balanced in terms of cold and hot properties, avoiding the damage to the spleen and stomach of aquatic animals caused by long-term feeding of traditional cold-natured medicinal materials. It can be added to feed regularly throughout the entire aquaculture cycle, meeting the requirements of green antibiotic-free aquaculture, and there are no drug residues or withdrawal period restrictions. Furthermore, it can achieve bidirectional synergistic regulation of the liver and intestines, with comprehensive target action. In addition, the composition provided by this invention can not only enhance the stress resistance of aquatic animals and reduce the incidence of aquaculture diseases, but also improve digestive and absorptive functions, thereby increasing the efficiency of aquaculture production.

The application belongs to the technical field of microorganisms, and specifically discloses a Candida utilis, a fermentation method and application thereof. The classification and naming of the strain is Candida utilis, which has been preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC NO:33529. The Candida utilis disclosed in the application can efficiently utilize various low-value industrial and agricultural by-products as carbon sources, and the crude protein content of the dry weight of the fermentation bacteria can be stably above 50%. In addition, the strain has excellent tolerance, can stably grow in the range of pH 3.5-6.0, and can tolerate an ammonium sulfate concentration as high as 15%. The strain of the application solves the problems of low protein yield and narrow substrate utilization spectrum of the yeast strain in the prior art, and provides important technical support for low-cost and high-efficiency production of single-cell protein.

This invention discloses a method for preparing ceramide 3 by acylation catalyzed by a composite microbial enzyme. The method uses plant sphingosine and mixed long-chain fatty acids as raw materials, and a binary composite microbial lipase with *Candida albicans* lipase as the main enzyme and *Aspergillus oryzae* / *Rhizopus oryzae* lipase as the coenzyme as the catalyst. The enzyme-catalyzed acylation reaction is followed by filtration, vacuum distillation, recrystallization, and drying to obtain the final product. This invention's composite enzyme system achieves highly efficient synergistic catalysis of the mixed fatty acid substrate, resulting in a ceramide 3 yield ≥90%, total purity ≥93%, and oleic acid-based ceramide 3 as the main component ≥85%. The distribution of minor components closely matches that of natural ceramides. The reaction conditions are mild, with no chemical residues. The composite enzyme is recyclable and reusable. The preparation process is simple, has high tolerance for error, and is easily scaled up industrially. The product can be widely used in the pharmaceutical and cosmetic fields.

This invention discloses a method for resolving the stereoisomers of 4-(trifluoromethyl)cyclohexane-1-ol, comprising first dissolving a mixture of stereoisomers of 4-(trifluoromethyl)cyclohexane-1-ol and an acyl donor in an organic solvent, and then reacting in the presence of Candida antarcticis lipase B to obtain trans-4-(trifluoromethyl)cyclohexane-1-ol and a cis-esterified product. This invention achieves the resolution of trans-4-(trifluoromethyl)cyclohexol from a mixture of 4-(trifluoromethyl)cyclohexol stereoisomers, and further obtains the cis-esterified product. The Candida antarcticis lipase B used in this invention is a more environmentally friendly biological enzyme with high product yield and mild reaction conditions. When used in immobilized form, Candida antarcticis lipase B is also easy to recover and reuse, which helps reduce production costs and has high production utilization value.