270 results about "Candida candida" patented technology

The invention provides a high-efficiency compound microbial inoculum for fermenting beds, particularly relates to a compound microbial inoculum for the fermenting beds, and a preparation method and application thereof. The compound microbial inoculum is composed of five beneficial bacterium strains (Bacillus subtilis CGMCC1.769 and CGMCC1.210, lactobacillus plantarum CICC6073, Candida tropicalis CGMCC2.587 and Trichoderma koningii CGMCC3.4290) and metabolites thereof (amylase, protease, lipase, cellulase, organic acid, bacteriophage and the like). The compound microbial inoculum is prepared by using a submerged liquid mixed fermentation technique. The method comprises the following steps: carrying out liquid submerged fermentation on the lactobacillus plantarum at 35 DEG C for 12 hours, respectively inoculating Candida tropicalis and Trichoderma koningii, carrying out mixed fermentation at 30 DEG C for 12 hours, inoculating Bacillus subtilis, carrying out liquid submerged mixed fermentation for 24 hours, respectively controlling the fermented viable count at 1-2*10<9> cfu / mL, 5-8*10<8> cfu / mL, 3-5*10<8> cfu / mL, 3-5*10<9> cfu / mL and 2-5*10<9> cfu / mL, finishing fermentation, packaging and sealing to obtain the high-efficiency compound microbial inoculum for the fermenting beds. The invention also provides application of the high-efficiency compound microbial inoculum-a preparation method of padding material. The compound microbial inoculum has the advantages of high viable count, high biological activity and low impurity bacterium pollution; the compound microbial inoculum can obviously reduce the odor of pigsties and reduce the living possibility of mosquitoes and flies, thereby achieving the ecological culture goals of no pollution and zero discharge; and the process for manufacturing the ferment bed is simple, thereby saving the padding material, human power and the like.

The invention relates to a multi-strain microorganism pig feed additive and a production method thereof. According to the additive, bacillus subtilis, lactobacillus delbrueckii, saccharomyces cerevisiae, candida and photosynthetic bacteria are inoculated into a fermentation culture medium according to a ratio of 2:1:1:1:2, and are prepared into a finished product through solid fermentation. The microorganism preparation is reasonably matched, various microorganisms in the preparation can bring respective effects into play and depend on one another, the overall advantage of the microorganism preparation can be brought into play, the growth development and the improvement of the anti-disease capability of a host are facilitated, and the feed additive is safe to use and can improve the immunity of animals, prevent and resist diseases, prompt animal growth, improve the feed utilization rate and improve the feed quality.

The invention relates to an apple pomace microbial culture starter, its preparation method and a method for producing biological feed by the apple pomace microbial culture starter. The apple pomace microbial culture starter comprises 1-15% of candida, 1-15% of geotrichum candidum, 1-15% of Aspergillus niger, 1-15% of Aspergillus oryzae and 1-15% of bacillus subtilis. The method utilizes a pomace multi-bacterium synergistic biological fermentation technology. The feed culture starter contains acid-forming bacteria, cellulose decomposition bacteria, protein production bacteria and disease-resistant health care bacteria and through synergism of the bacteria, a pomace conversion utilization rate is improved, a digestion rate is improved, crude fiber content is reduced and crude protein content is improved. The biological feed prepared by the apple pomace microbial culture starter contains comprehensive nutrients, is green and safe, has a low cost and high benefits, substantially reduces hoof diseases of female animals bred in the feeding farm, improves a reproductive rate, is suitable for large scale and industical production and has a wide application prospect.

The invention provides a composition, a composite bacterium agent and application thereof. The composition or the composite bacterium agent comprises a composition of at least six or seven strains from bacillus subtilis, bacillus megatherium, pseudomonad, sphingobacterium thalpophilum, candida tropicalis, bacillus firmus and lactobacillus plantarum. The application includes sludge treatment.

Food containing γ-glutamylcysteine or cysteine is produced by culturing Candida utilis containing 1% by weight or more of γ-glutamylcysteine per dry cells in logarithmic growth phase when cultured in a minimal medium, for example, Candida utilis in which a gene encoding glutathione synthetase is modified so that intracellular glutathione synthetase activity is reduced, under a suitable condition and mixing the obtained culture or a fraction thereof or the culture or a fraction thereof subjected to heat treatment with a raw material of food or drink to process food or drink.

The invention provides a nucleotide sequence suitable for secretory expression of recombinant candida utilis uricase (uricase, EC1.7.3.3) by virtue of escherichia coli, a carrier containing the nucleotide sequence, an engineering cell containing the carrier, and a method for purifying the recombinant candida utilis uricase from the engineering cell. On the basis of a natural sequence of a uricase gene, a codon of the uricase gene is optimized, the transformed uricase gene is cloned to a prokaryotic expression vector pET42a(+); an escherichia coli Rossetta strain is transformed; the expression level can reach 50% of soluble protein of the escherichia coli by IPTG induction; the expressed protein is soluble protein; and the biological activity of the expressed protein can be up to that of 37.89U / mg recombinant protein. The recombinant candida utilis uricase product can be efficiently and simply obtained at low cost. The invention also comprises an application of the recombinant candida utilis uricase prepared by the method in preparation of medicines, foods, health care products and cosmetics for treating hyperuricemia-related diseases or pathological symptoms of a human body.

The invention relates to a fungus agent for reducing mildew of fermentation feed and mycotoxin harm and application. The microbial agent for reducing mildew of fermentation feed and mycotoxin harm comprises the following effective components: Bacillus subtilis, Candida utilis, lactic acid bacteria, compound enzyme preparation, glucose and peptone. According to the present invention, Bacillus subtilis Bsbc01 and Candida utilis are used together for the first time to prepare the fungus agent, Candida utilis has the advantages of simple required nutrients, fast growth and reproduction, etc., canquickly consume the oxygen present in the feed gap and limit the growth and reproduction of mold, and at the same time, Candida utilis has the characteristics of high protein and vitamin B content, etc., which can increase the nutritional value of feed.

The invention discloses a microbial agent for garbage composting as well as a preparation method and application thereof. The microbial agent comprises bacillus amyloliquefaciens, candida utilis, lactobacillus acidophilus and rhodopseudomonas palustris and is a liquid bacterial agent or solid bacterial agent. The microbial agent for garbage composting, disclosed by the invention, has the advantages of easiness in production, stable product quality, low cost and strong controllability in a garbage composting process; after the microbial agent is added into the garbage, the content of beneficial efficient microorganisms in the garbage can be increased, the microbial activity is improved, the garbage fermentation process is accelerated, the leaching of water and the increase of the lower calorific value of garbage are promoted, and the incineration efficiency of the garbage is improved; in a region with relatively low winter temperature, the microbial agent can reduce the influence caused by the low-temperature environment change so as to guarantee smooth operation of the garbage incineration according to the technology design; and by adopting the microbial agent, the generation of garbage smell can be reduced, and the working environment is improved.