179 results about "Candida candida" patented technology

The invention discloses a Candida antarctica lipase B mutant with high transesterification activity and application thereof. The nucleotide sequence of the Candida antarctica lipase B mutant is shown as SEQ ID NO.1, and the amino acid sequence of the expression protein of the Candida antarctica lipase B mutant is shown as SEQ ID NO.2. The invention further discloses a preparation method of the Candida antarctica lipase B mutant. Compared with an original enzyme, the mutant has higher fat hydrolysis activity and transesterification activity, the specific enzyme activity of the fat hydrolysis activity reaches 480.39 U / mg, the specific enzyme activity of the transesterification activity reaches 40.67 U / mg, the biodiesel conversion rate at the temperature of 30 DEG C can reach 92%, low-temperature high catalytic activity is shown, and the mutant can be used for enzymatic preparation of biodiesel and has potential industrial application value.

The invention discloses a microbial compound feed additive, which is prepared from the following ingredients in parts by weight: 35 to 45 parts of traditional Chinese medicine residues, 25 to 35 parts of soybean meal, 15 to 25 parts of bran, 8 to 12 parts of corn flour, 0.3 to 0.7 part of selenium-enriched yeast, 8 to 12 parts of aspergillus niger spore powder, 10 to 15 parts of bacillus subtilis, 6 to 9 parts of lactobacillus plantarum and 5 to 8 parts of candida utilis. The invention relates to the technical field of feed additives, through the synergistic effect of the aspergillus niger, the bacillus subtilis, the lactobacillus plantarum and the candida utilis, the aspergillus niger can generate various enzymes, and decomposition of raw materials and release of nutritional ingredients are facilitated; the bacillus subtilis can enhance the immunity of livestock and poultry and inhibit the growth of harmful bacteria; lactobacillus plantarum can regulate intestinal microecological balance and promote digestion and absorption; candida utilis is rich in protein and vitamins and provides nutrition for livestock and poultry, the four components cooperate with each other, the function limitation of a single strain is broken through, and the health level and production performance of livestock and poultry are comprehensively improved.

The invention discloses high-stability immobilized candida antarctica lipase B as well as a preparation method and application thereof, and belongs to the technical field of enzyme engineering and biological catalysis. The preparation method comprises the following steps: modifying resin by adopting a silane coupling agent to obtain silanized resin; carrying out aldehyde functional modification on the silanized resin by using dialdehyde cellulose to obtain alkyl-aldehyde modified resin; candida antarctica lipase B is immobilized by using alkyl-aldehyde modified resin, and after the Candida antarctica lipase B is subjected to interfacial activation on the surface of the alkyl-aldehyde modified resin, the Candida antarctica lipase B is covalently bound with aldehyde on the surface of the alkyl-aldehyde modified resin; the prepared immobilized Candida antarctica lipase B has relatively high activity and stability. The method is simple to operate, the prepared immobilized lipase is high in stability, and the method can be used for preparing the high-stability immobilized lipase on a large scale and has the potential of industrial popularization and application.

The invention discloses candida tropicalis with the effect of reducing nitrite and ammonia nitrogen and application of the candida tropicalis. The saccharomycetes are respectively named as candida tropicalis JM-2, and the preservation number of the candida tropicalis JM-2 is GDMCC No: 64449. The saccharomyces cerevisiae is high in adaptability, grows well in high-concentration ammonia nitrogen and nitrite environments, is relatively high in nitrogen degradation efficiency, can be used as a functional strain for improving water quality, deodorizing farms, deodorizing garbage yards and the like, can be applied to a plurality of places, effectively degrades harmful substances such as nitrite, ammonia nitrogen and the like in water bodies and environments, reduces nitrogen accumulation, protects the environments, and has a wide application prospect. The method has great application potential in livestock and poultry aquaculture and environmental protection.

The invention discloses a kitchen waste oil-based sophorolipid high-yield candida utilis strain and an application thereof. Belongs to the technical field of directional domestication breeding. According to the method, wild candida bombicola is used as an original strain, and is sequentially domesticated in a culture medium containing kitchen waste oil with the concentration gradient of 2%, 4%, 6%, 8% and 10% to obtain the S.bombicola x10. The invention further discloses an application of the domesticated strain in fermentation production of sophorolipid, after the strain is subjected to shake flask fermentation, the sophorolipid yield reaches 60.19 g / L and is improved by 16.22% compared with that of a wild strain, and the acid type sophorolipid yield is improved by 32.11%. When the concentration of the lactone sophorolipid generated by fermentation is 1.25 mg / mL, the growth of the bacillus subtilis is completely inhibited. The invention provides an excellent production strain for industrial production of sophorolipid, and has good theoretical research significance and practical application value.

The invention relates to screening and application of a Candida antarctica lipase B mutant. Specifically, the Candida antarctica lipase B mutant provided by the invention is obtained by carrying out rational design and directed evolution combined strategy mutation on the basis of a wild Candida antarctica lipase B gene sequence, and the amino acid sequence of the mutant is shown as SEQ ID NO.3. The invention also relates to a preparation method of the Candida antarctica lipase B mutant. The mutant has higher enzyme activity and thermal stability, the hydrolytic catalytic activity of the mutant is improved to 2.1 times of the original hydrolytic catalytic activity, and the half-life period of the mutant enzyme at 50 DEG C is prolonged by 4 times. On the basis, pichia pastoris is used as a host to express the mutant, and a strain with hydrolytic activity improved by 52% is obtained through high resistance screening. The lipase is prepared into an immobilized enzyme to be used in a synthetic reaction of chlorogenic acid derivatives, the highest conversion rate of chlorogenic acid reaches 74% in 24 hours, and the immobilized enzyme has a good industrial application prospect.

The invention provides application of candida tropicalis and a preparation thereof in prevention and treatment of tobacco mildew, and belongs to the technical field of microorganisms. A strain of candida tropicalis is named as candida tropicalis 2-17, the preservation number of the candida tropicalis 2-17 is CCTCC NO: M 20242184, a microbial preparation prepared from the candida tropicalis can inhibit growth of main mould in tobacco leaves, and the candida tropicalis has good application potential for biological prevention and treatment of tobacco leaf mould.

The invention provides a method for preparing quintuple diglyceride oil for improving blood vessel health through enzymolysis, application and a product, and belongs to the technical field of enzyme engineering. The preparation method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol, water, fatty acid and lipase, and carrying out enzymolysis reaction, molecular distillation, decoloration and deodorization to obtain diglyceride oil; the lipase is a mixture of Candida antarctica Aspergillus niger lipase, Candida column lipase and bovine pancreas lipase, and the weight ratio of the low-erucic acid rapeseed oil to the glycerin to the water to the fatty acid to the lipase is 500: (80-100): (1-2): (30-60): (10-20). The diglyceride oil prepared by the method can be used for preparing a diglyceride oil composition, and has five functions of reducing blood uric acid, reducing blood total cholesterol, reducing blood triglyceride, reducing blood sugar and increasing blood flow velocity to improve blood vessel health.

The invention discloses a method for purifying modified sophorolipid, which comprises the following steps: adding a salt solution into lactone type crude sophorolipid obtained by fermenting candida bumblebee, stirring, standing, and collecting a lower layer sophorolipid phase; according to the method, the produced crude sophorolipid with low or high emulsification degree is purified, impurities are removed, good appearance conditions are achieved, and the appearance requirement of the commodity sophorolipid is met. And meanwhile, the sophorolipid is modified in the purification process by utilizing the chemical properties of different salts, so that the decoloration of the sophorolipid is realized, the concentration of the lactone type sophorolipid is improved, and the stability of the lactone type sophorolipid is improved at the same time.

The invention discloses a lipase A mutant as well as a preparation method and application thereof, and belongs to the technical field of biology. The lipase A mutant is a protein obtained by carrying out one or more amino acid residue changes on at least one of the following sites of an amino acid sequence as shown in SEQ ID NO: 1: the 94th site, the 117th site, the 121st site, the 122nd site, the 283rd site and the 431st site; wherein the change is selected from at least one of substitution, deletion and addition. Through rational design, lipase A from Candida antarctica is subjected to site-directed mutagenesis, the Candida antarctica lipase A mutant with catalytic activity and selectivity to the sn-2 site of triglyceride remarkably improved is obtained, and when 1, 3-diglyceride is prepared, the Candida antarctica lipase A mutant can remarkably improve the yield of 1, 3-diglyceride. The yield of the 1, 3-diglyceride is improved.

The invention provides diglyceride oil with a blood sugar reducing effect and a preparation method of the diglyceride oil, and relates to the technical field of functional grease processing. The preparation method comprises the following steps: mixing sea buckthorn seed oil, acer truncatum seed oil, rapeseed oil, corn oil and high oleic acid peanut oil, and adding glycerol and water to obtain a material A; adding onion pseudomonas lipase and candida antarctica lipase B into the material A, and carrying out primary reaction to obtain a material B; adding candida lipolytica lipase and candida antarctica lipase B into the material B, and carrying out a second reaction to obtain a material C; adding thermomyces lanuginosus lipase into the material C, and carrying out a third reaction to obtain a material D; and treating the reacted material D to obtain the diglyceride oil. The diglyceride oil prepared by the invention has a good effect in the aspect of reducing blood sugar.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a fermentation strain composition, fermentation oil as well as a preparation method and application of the fermentation oil. The raw materials of the fermented oil comprise grease, candida bumblebee and a second strain; and the second strain is selected from at least one of lipomyces starkeyi, saccharomyces cerevisiae, rhodosporidium torulosporioides, lucid ganoderma and cordyceps sobolifera. The preparation method comprises the following steps: firstly, adding grease into a culture medium, inoculating bumblebee candida and a second strain, and fermenting; and sterilizing and filtering after fermentation. The invention provides a grease raw material fermentation strain combination capable of improving the grease stability, remarkably reducing the dosage of a grease emulsifier and improving the transdermal absorption effect of grease as an active matter carrier, and provides a basis for the application of the grease raw material fermentation strain combination in cosmetics.

The invention relates to the technical field of biocatalytic synthesis of fatty glyceride, in particular to a flaky zeolite immobilized Candida antarctica lipase B catalyst and a method for preparing medium-chain glyceride through catalytic esterification of the flaky zeolite immobilized Candida antarctica lipase B catalyst. The flaky zeolite is flaky silica-alumina zeolite ZSM-5-P, and the loading capacity of the candida antarctica lipase B is 9-12% of the mass of the flaky zeolite. The candida antarctica lipase B is loaded and fixed in the flaky zeolite to prepare the solid catalyst, and the catalyst is a solid enzyme biocatalyst, is small in usage amount, high in catalytic efficiency and recyclable, is used for preparing the medium-chain glyceride, is high in efficiency, low in cost, simple in process, mild in condition, safe and environment-friendly, and has a wide application prospect.

The invention discloses diglyceride prepared by an enzymic method as well as a preparation method and application thereof, and belongs to the technical field of deep processing of grease. The method comprises the following steps: adding fatty acid into a choline chloride-glycerol-urea ternary eutectic solvent, uniformly mixing, and then adding candida antarctica lipase B for esterification reaction to obtain a reaction mixture; separating the reaction mixture, and recovering an upper-layer oil phase; and separating and purifying the upper-layer oil phase to obtain the diglyceride. According to the method, the reaction rate is high, the selectivity of the diglyceride in the esterification process is high, the yield of the diglyceride can reach 65% or above by reacting for 1 h, the selectivity of the diglyceride is 75% or above, and the purity of the separated and purified diglyceride is 90% or above; in addition, the method is simple to operate, and the reaction product and the eutectic solvent can be separated through simple centrifugation after the reaction is finished; in addition, the monoglyceride content in the product is low, and the subsequent separation and purification difficulty is greatly reduced.

The invention belongs to the technical field of functional grease processing, and particularly relates to an enzymolysis preparation method and application of diglyceride with an effect of improving Alzheimer's disease. The method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol and water, and heating to obtain a pre-reaction mixture; adding compound enzyme for reaction, and then adding candida antarctica lipase B and pseudomonas fluorescens lipase for reaction; and after the reaction is completed, carrying out molecular distillation, collecting a light-phase product, carrying out adsorption filtration, and collecting filtrate to obtain the diglyceride oil. The compound enzyme is composed of rhizomucor miehei lipase, candida rugosa lipase and candida antarctica lipase B in a weight ratio of (2-4): 2: 1. According to the method, the content of diglyceride is effectively increased, the prepared composition has the efficacy of preventing and treating Alzheimer's disease, and the efficacy is shown as improvement of reaction capacity and cognitive function, inhibition of brain apoptosis and acetylcholin esterase, enhancement of SOD and CAT vitality and improvement of related gene expression.

The invention discloses a bumblebee candida for efficiently producing sophorolipid as well as a construction method and application of the bumblebee candida. According to the invention, metabolic transformation is carried out on a wild type strain of candida bumblebee, a peroxisome membrane transporter gene PXA1 is knocked out, so that a beta-oxidation pathway of fatty acid competing with a sophorolipid synthesis pathway is moderately inhibited, and on the basis, an endogenous potent promoter pENO is utilized to enhance expression of CYP52M1; therefore, the bumblebee candida engineering bacteria for efficiently producing sophorolipid are constructed. Compared with an original strain, the recombinant candida bumblebee has the advantages that efficient conversion of a fermentation substrate and high yield of sophorolipid can be realized, and the sophorolipid yield of shake flask fermentation reaches 88.50 g / L. The strain is fermented in a 5L fermentation tank and fermented for 7 days after rapeseed oil is added, 232.27 g / L of crude sophorolipid is produced, and high productivity is kept.

The invention discloses candida tropicalis engineering bacteria for high yield of (-)-alpha-bisabolol and a construction method of the candida tropicalis engineering bacteria, and belongs to the technical field of microorganisms. According to the invention, chassis cell candida tropicalis DC04-WG04-2 is constructed, a gene Cat2 of catalase derived from saccharomyces cerevisiae and a gene ERG10 of 3-hydroxy-3-methylglutaryl coenzyme A reductase are expressed on the basis of the chassis cell candida tropicalis DC04-WG04-2, and a PEX15 motif is anchored on the surface of peroxisome for co-expression; the genes ADH1, GPD1 and GPD2 are further knocked out, so that the accumulation of ethanol is reduced, and the generation of glycerol is reduced. According to the method disclosed by the invention, the yield of the (-)-alpha-bisabolol of the recombinant candida tropicalis reaches 461.7 mg / L by optimizing fermentation conditions. The preparation method has the characteristics of low cost, high efficiency, simplicity in operation, wide prospect and the like.

The present invention relates to a strain of Candida and its application, belonging to the technical field of microbial fermentation. The Candida SY152 provided by the present invention is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration Committee, with a deposit number of CGMCC No.31427, a deposit date of July 24, 2024, a deposit address of Beijing, China, and is classified and named as tropical Candida Candida tropicalis The Candida SY152 provided by the present invention can increase the pH value of the culture medium, and when used for fermentation of soybean paste or soy sauce, it can significantly reduce the total acid content in the soybean paste or soy sauce, while increasing the ethanol content, thereby increasing the generated alcohol and ester substances, thereby enhancing the mellow flavor of the finished soybean paste or soy sauce, and significantly improving its taste and flavor.

The invention relates to candida tropicalis and application thereof, the candida tropicalis is a candida tropicalis JSY83 strain, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20241913, and the preservation date is September 4, 2024. The strain has excellent ethanol, acetic acid and lactic acid tolerance, especially has more excellent lactic acid tolerance, and ensures the growth and functional effects of the strain in the actual white spirit brewing process; by inhibiting the growth of ester-producing yeast, reducing the accumulation of acetic acid, reducing the content of acetic acid in fermented grains and inhibiting the accumulation of ethyl acetate in the fermented grains, the method has important significance on improving the high-quality product rate and the liquor yield of raw liquor when being used for producing yeast for making hard liquor and Luzhou-flavor liquor.

The invention discloses a culture base material for lepista sordida rich in strontium, zinc and germanium as well as a preparation method and application of the culture base material, and relates to the technical field of edible mushrooms. The culture base stock is prepared from the following raw materials: a nitrogen source, a carbon source, inorganic salt, spirulina, polyglutamic acid and compound bacteria, the compound bacteria comprise aspergillus niger, lactobacillus cavings-like and candida utilis; the culture base stock comprises trace elements; the trace elements comprise at least one of strontium, zinc and germanium. The culture base stock can effectively shorten the growth cycle of lepista sordida, improve the yield and the biotransformation rate, reduce the cost and promote enrichment of trace elements such as strontium, zinc and germanium in lepista sordida.

The invention relates to a method for preparing fish feed by secondary utilization of traditional Chinese medicine wastes, and belongs to the technical field of fish feed. After the traditional Chinese medicine waste is pretreated to remove heavy metal, primary fermentation of bacillus subtilis and aspergillus niger is carried out, the bacillus subtilis can degrade cellulose, the aspergillus niger decomposes antinutritional factors, then fermentation of lactobacillus lactis and candida utilis is carried out, lactic acid bacteria produce acid to inhibit infectious microbes, flavor substances are synthesized, and palatability is improved. Through traditional Chinese medicine residue pretreatment, multi-strain synergistic fermentation and a scientific feed formula, efficient reutilization of traditional Chinese medicine waste is successfully realized, and the fish feed which is rich in nutrition, high in safety and remarkable in economic benefit is produced.

Disclosed is a method of reducing and / or removing diglyceride content in an oil without substantial transesterification of triglycerides, the method comprising the steps of: providing an oil or fat; and hydrolyzing the monoglycerides and diglycerides in the oil with water in the presence of a lipase having at least 80% sequence identity to SEQ ID NO: 1.

The present invention belongs to the field of biochemical engineering technology, and specifically relates to a method for producing octadecadicarboxylic acid by fermenting Candida visweiss. The method for producing octadecadicarboxylic acid by fermenting Candida visweiss described in the present invention comprises the following steps: (1) transferring the Candida visweiss seed liquid into a fermentation tank with a prepared culture medium for culturing; (2) adding substrate in stages or continuously during the fermentation process; (3) collecting the fermentation liquid after the fermentation is completed, extracting octadecenoic acid, and then hydrogenating it to obtain octadecadicarboxylic acid; the Candida visweiss has a preservation number of CCTCC NO: M 20222052, which is preserved in the China Center for Type Culture Collection, with the preservation address at Wuhan University, the preservation date being December 23, 2022, and the Latin name is Candida viswanathii The method of the present invention has high conversion efficiency, high product purity and high yield.

The invention discloses candida utilis engineering bacteria and a method for producing single-cell protein by fermenting corn thin pulp, and belongs to the technical field of biology. According to the candida utilis engineering bacteria disclosed by the invention, the candida utilis is subjected to gene modification, a chitin synthase gene in the candida utilis is knocked out, and an exogenous keratinase gene is integrated, so that the candida utilis engineering bacteria can utilize corn thin pulp as a substrate for fermentation. In the fermentation process, carbon and nitrogen sources in the thin corn pulp can be effectively utilized for growth, macromolecular protein in the thin corn pulp can be hydrolyzed and converted into single-cell protein, the content of the single-cell protein in final fermentation liquor is increased, the protein content of the final fermentation liquor can reach 60% or above, and the proportion of the single-cell protein can reach 30% or above; the corn thin pulp can be efficiently utilized, and meanwhile, single-cell protein in the fermentation liquor can be used for adding animal feed so as to meet the requirement for animal protein feed in the market.

The application belongs to the field of microorganism and fermentation engineering, and provides a Candida magnoliae with high yield of 3-methylthiopropanol, and the preservation number is CGMCC NO. 23668. The Candida magnoliae can be used for producing 3-methylthiopropanol, the yield can reach 3.16 g / L, and the ethanol tolerance is high, and can be applied in the wine making, condiment and food additive industries.

The invention relates to the technical field of microbial fermentation, and provides a method for producing single-cell protein, which comprises the following steps: inoculating a crop straw matrix with a first microbial seed for first fermentation; the first microbial seed comprises trametes versicolor; sterilizing a fermentation product obtained by the first fermentation; inoculating second microbial seeds into the sterilized fermentation product for second fermentation; the second microbial seed comprises yarrowia lipolytica / candida utilis. According to the scheme, the combination of the selected first microbial seed solution and the second microbial seed solution has a remarkable synergistic effect, so that the crude protein content, the lignin removal rate, the cellulose retention rate and the yeast biomass are improved, and the hemicellulose loss rate is reduced.

The invention belongs to the technical field of microorganisms, and discloses bacillus subtilis with a high nattokinase yield and a biological preservation function and application of the bacillus subtilis. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34222. The serine protease gene of the strain carries specific mutations (SEQ ID NO: 1) which are mutated from G to C at the 217th site and the 230th site, so that the yield and the activity of nattokinase are remarkably improved. In the fermentation process, through an induction stage (38 DEG C heat preservation for 8 hours), a main fermentation stage (50 DEG C heat preservation for 7 hours) and a cooling after-ripening stage (4 DEG C heat preservation for 24 hours), the enzyme activity of the prepared natto product is larger than or equal to 57028 IU / mL, and the natto product is light in flavor, excellent in wiredrawing effect and appropriate in taste. In addition, the strain can inhibit the growth of Candida albicans (the inhibition zone is greater than or equal to 25mm), and the shelf life of natto is effectively prolonged. The problems that an existing strain is poor in stability, products are prone to pollution, and the flavor is poor are solved.

The invention belongs to the technical field of biology and the technical field of fermentation, and relates to a compound microbial agent and application thereof in regulating and controlling the fermentation temperature of a saccharification pile. The compound microbial agent is prepared from bacillus coagulans, bacillus subtillis, pichia kudriavzevii, candida tropicalis, saccharomyces cerevisiae, high-temperature Daqu actinomycetes and common high-temperature actinomycetes, and the compound microbial agent is used for saccharification and stacking fermentation, so that the stacking fermentation temperature is increased, and the stacking time is shortened.

1. Name of the designed product: packing bag (NFP50 feed raw material Candida utilis protein). 2. Use of the designed product: used for packing feed. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: front view. 5. The designed product is a thin product, and the bottom view, top view, left view and right view are omitted.

The invention belongs to the technical field of directed evolution modification and biological catalysis application of enzymes, and relates to a Candida antarctica lipase B mutant protein and application thereof, the sequence of the mutant protein comprises one or more of the mutation of the 195 S and F, the mutation of the 195 S and Y and the mutation of the 283 A and Y from the N terminal to the C terminal of the amino acid sequence shown in SEQ ID NO.1 of the original CALB; on the basis of a wild type lipase gene sequence, through rational design and site-specific mutagenesis, mutants S195F, S195Y, A283Y, S195F-A283Y and S195Y-A283Y, of which the Tm value and the optimal reaction temperature are obviously improved and the catalytic activity is improved by 1-2 times, are finally obtained; when the mutant is used for catalyzing synthesis of docosahexaenoic acid (DHA) glyceride, higher catalytic efficiency is shown, and the yield of the DHA glyceride is effectively improved.