124 results about "Candida candida" patented technology

The invention discloses a microbial compound feed additive, which is prepared from the following ingredients in parts by weight: 35 to 45 parts of traditional Chinese medicine residues, 25 to 35 parts of soybean meal, 15 to 25 parts of bran, 8 to 12 parts of corn flour, 0.3 to 0.7 part of selenium-enriched yeast, 8 to 12 parts of aspergillus niger spore powder, 10 to 15 parts of bacillus subtilis, 6 to 9 parts of lactobacillus plantarum and 5 to 8 parts of candida utilis. The invention relates to the technical field of feed additives, through the synergistic effect of the aspergillus niger, the bacillus subtilis, the lactobacillus plantarum and the candida utilis, the aspergillus niger can generate various enzymes, and decomposition of raw materials and release of nutritional ingredients are facilitated; the bacillus subtilis can enhance the immunity of livestock and poultry and inhibit the growth of harmful bacteria; lactobacillus plantarum can regulate intestinal microecological balance and promote digestion and absorption; candida utilis is rich in protein and vitamins and provides nutrition for livestock and poultry, the four components cooperate with each other, the function limitation of a single strain is broken through, and the health level and production performance of livestock and poultry are comprehensively improved.

The invention discloses a kitchen waste oil-based sophorolipid high-yield candida utilis strain and an application thereof. Belongs to the technical field of directional domestication breeding. According to the method, wild candida bombicola is used as an original strain, and is sequentially domesticated in a culture medium containing kitchen waste oil with the concentration gradient of 2%, 4%, 6%, 8% and 10% to obtain the S.bombicola x10. The invention further discloses an application of the domesticated strain in fermentation production of sophorolipid, after the strain is subjected to shake flask fermentation, the sophorolipid yield reaches 60.19 g / L and is improved by 16.22% compared with that of a wild strain, and the acid type sophorolipid yield is improved by 32.11%. When the concentration of the lactone sophorolipid generated by fermentation is 1.25 mg / mL, the growth of the bacillus subtilis is completely inhibited. The invention provides an excellent production strain for industrial production of sophorolipid, and has good theoretical research significance and practical application value.

The invention relates to screening and application of a Candida antarctica lipase B mutant. Specifically, the Candida antarctica lipase B mutant provided by the invention is obtained by carrying out rational design and directed evolution combined strategy mutation on the basis of a wild Candida antarctica lipase B gene sequence, and the amino acid sequence of the mutant is shown as SEQ ID NO.3. The invention also relates to a preparation method of the Candida antarctica lipase B mutant. The mutant has higher enzyme activity and thermal stability, the hydrolytic catalytic activity of the mutant is improved to 2.1 times of the original hydrolytic catalytic activity, and the half-life period of the mutant enzyme at 50 DEG C is prolonged by 4 times. On the basis, pichia pastoris is used as a host to express the mutant, and a strain with hydrolytic activity improved by 52% is obtained through high resistance screening. The lipase is prepared into an immobilized enzyme to be used in a synthetic reaction of chlorogenic acid derivatives, the highest conversion rate of chlorogenic acid reaches 74% in 24 hours, and the immobilized enzyme has a good industrial application prospect.

The invention provides a method for preparing quintuple diglyceride oil for improving blood vessel health through enzymolysis, application and a product, and belongs to the technical field of enzyme engineering. The preparation method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol, water, fatty acid and lipase, and carrying out enzymolysis reaction, molecular distillation, decoloration and deodorization to obtain diglyceride oil; the lipase is a mixture of Candida antarctica Aspergillus niger lipase, Candida column lipase and bovine pancreas lipase, and the weight ratio of the low-erucic acid rapeseed oil to the glycerin to the water to the fatty acid to the lipase is 500: (80-100): (1-2): (30-60): (10-20). The diglyceride oil prepared by the method can be used for preparing a diglyceride oil composition, and has five functions of reducing blood uric acid, reducing blood total cholesterol, reducing blood triglyceride, reducing blood sugar and increasing blood flow velocity to improve blood vessel health.

The invention discloses diglyceride prepared by an enzymic method as well as a preparation method and application thereof, and belongs to the technical field of deep processing of grease. The method comprises the following steps: adding fatty acid into a choline chloride-glycerol-urea ternary eutectic solvent, uniformly mixing, and then adding candida antarctica lipase B for esterification reaction to obtain a reaction mixture; separating the reaction mixture, and recovering an upper-layer oil phase; and separating and purifying the upper-layer oil phase to obtain the diglyceride. According to the method, the reaction rate is high, the selectivity of the diglyceride in the esterification process is high, the yield of the diglyceride can reach 65% or above by reacting for 1 h, the selectivity of the diglyceride is 75% or above, and the purity of the separated and purified diglyceride is 90% or above; in addition, the method is simple to operate, and the reaction product and the eutectic solvent can be separated through simple centrifugation after the reaction is finished; in addition, the monoglyceride content in the product is low, and the subsequent separation and purification difficulty is greatly reduced.

The invention belongs to the technical field of functional grease processing, and particularly relates to an enzymolysis preparation method and application of diglyceride with an effect of improving Alzheimer's disease. The method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol and water, and heating to obtain a pre-reaction mixture; adding compound enzyme for reaction, and then adding candida antarctica lipase B and pseudomonas fluorescens lipase for reaction; and after the reaction is completed, carrying out molecular distillation, collecting a light-phase product, carrying out adsorption filtration, and collecting filtrate to obtain the diglyceride oil. The compound enzyme is composed of rhizomucor miehei lipase, candida rugosa lipase and candida antarctica lipase B in a weight ratio of (2-4): 2: 1. According to the method, the content of diglyceride is effectively increased, the prepared composition has the efficacy of preventing and treating Alzheimer's disease, and the efficacy is shown as improvement of reaction capacity and cognitive function, inhibition of brain apoptosis and acetylcholin esterase, enhancement of SOD and CAT vitality and improvement of related gene expression.

The invention discloses a bumblebee candida for efficiently producing sophorolipid as well as a construction method and application of the bumblebee candida. According to the invention, metabolic transformation is carried out on a wild type strain of candida bumblebee, a peroxisome membrane transporter gene PXA1 is knocked out, so that a beta-oxidation pathway of fatty acid competing with a sophorolipid synthesis pathway is moderately inhibited, and on the basis, an endogenous potent promoter pENO is utilized to enhance expression of CYP52M1; therefore, the bumblebee candida engineering bacteria for efficiently producing sophorolipid are constructed. Compared with an original strain, the recombinant candida bumblebee has the advantages that efficient conversion of a fermentation substrate and high yield of sophorolipid can be realized, and the sophorolipid yield of shake flask fermentation reaches 88.50 g / L. The strain is fermented in a 5L fermentation tank and fermented for 7 days after rapeseed oil is added, 232.27 g / L of crude sophorolipid is produced, and high productivity is kept.

The invention discloses a culture base material for lepista sordida rich in strontium, zinc and germanium as well as a preparation method and application of the culture base material, and relates to the technical field of edible mushrooms. The culture base stock is prepared from the following raw materials: a nitrogen source, a carbon source, inorganic salt, spirulina, polyglutamic acid and compound bacteria, the compound bacteria comprise aspergillus niger, lactobacillus cavings-like and candida utilis; the culture base stock comprises trace elements; the trace elements comprise at least one of strontium, zinc and germanium. The culture base stock can effectively shorten the growth cycle of lepista sordida, improve the yield and the biotransformation rate, reduce the cost and promote enrichment of trace elements such as strontium, zinc and germanium in lepista sordida.

The invention relates to a method for preparing fish feed by secondary utilization of traditional Chinese medicine wastes, and belongs to the technical field of fish feed. After the traditional Chinese medicine waste is pretreated to remove heavy metal, primary fermentation of bacillus subtilis and aspergillus niger is carried out, the bacillus subtilis can degrade cellulose, the aspergillus niger decomposes antinutritional factors, then fermentation of lactobacillus lactis and candida utilis is carried out, lactic acid bacteria produce acid to inhibit infectious microbes, flavor substances are synthesized, and palatability is improved. Through traditional Chinese medicine residue pretreatment, multi-strain synergistic fermentation and a scientific feed formula, efficient reutilization of traditional Chinese medicine waste is successfully realized, and the fish feed which is rich in nutrition, high in safety and remarkable in economic benefit is produced.

The invention discloses candida utilis engineering bacteria and a method for producing single-cell protein by fermenting corn thin pulp, and belongs to the technical field of biology. According to the candida utilis engineering bacteria disclosed by the invention, the candida utilis is subjected to gene modification, a chitin synthase gene in the candida utilis is knocked out, and an exogenous keratinase gene is integrated, so that the candida utilis engineering bacteria can utilize corn thin pulp as a substrate for fermentation. In the fermentation process, carbon and nitrogen sources in the thin corn pulp can be effectively utilized for growth, macromolecular protein in the thin corn pulp can be hydrolyzed and converted into single-cell protein, the content of the single-cell protein in final fermentation liquor is increased, the protein content of the final fermentation liquor can reach 60% or above, and the proportion of the single-cell protein can reach 30% or above; the corn thin pulp can be efficiently utilized, and meanwhile, single-cell protein in the fermentation liquor can be used for adding animal feed so as to meet the requirement for animal protein feed in the market.

The application belongs to the field of microorganism and fermentation engineering, and provides a Candida magnoliae with high yield of 3-methylthiopropanol, and the preservation number is CGMCC NO. 23668. The Candida magnoliae can be used for producing 3-methylthiopropanol, the yield can reach 3.16 g / L, and the ethanol tolerance is high, and can be applied in the wine making, condiment and food additive industries.

The invention relates to the technical field of microbial fermentation, and provides a method for producing single-cell protein, which comprises the following steps: inoculating a crop straw matrix with a first microbial seed for first fermentation; the first microbial seed comprises trametes versicolor; sterilizing a fermentation product obtained by the first fermentation; inoculating second microbial seeds into the sterilized fermentation product for second fermentation; the second microbial seed comprises yarrowia lipolytica / candida utilis. According to the scheme, the combination of the selected first microbial seed solution and the second microbial seed solution has a remarkable synergistic effect, so that the crude protein content, the lignin removal rate, the cellulose retention rate and the yeast biomass are improved, and the hemicellulose loss rate is reduced.

The invention belongs to the technical field of microorganisms, and discloses bacillus subtilis with a high nattokinase yield and a biological preservation function and application of the bacillus subtilis. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34222. The serine protease gene of the strain carries specific mutations (SEQ ID NO: 1) which are mutated from G to C at the 217th site and the 230th site, so that the yield and the activity of nattokinase are remarkably improved. In the fermentation process, through an induction stage (38 DEG C heat preservation for 8 hours), a main fermentation stage (50 DEG C heat preservation for 7 hours) and a cooling after-ripening stage (4 DEG C heat preservation for 24 hours), the enzyme activity of the prepared natto product is larger than or equal to 57028 IU / mL, and the natto product is light in flavor, excellent in wiredrawing effect and appropriate in taste. In addition, the strain can inhibit the growth of Candida albicans (the inhibition zone is greater than or equal to 25mm), and the shelf life of natto is effectively prolonged. The problems that an existing strain is poor in stability, products are prone to pollution, and the flavor is poor are solved.

1. Name of the designed product: packing bag (NFP50 feed raw material Candida utilis protein). 2. Use of the designed product: used for packing feed. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: front view. 5. The designed product is a thin product, and the bottom view, top view, left view and right view are omitted.

The invention belongs to the technical field of directed evolution modification and biological catalysis application of enzymes, and relates to a Candida antarctica lipase B mutant protein and application thereof, the sequence of the mutant protein comprises one or more of the mutation of the 195 S and F, the mutation of the 195 S and Y and the mutation of the 283 A and Y from the N terminal to the C terminal of the amino acid sequence shown in SEQ ID NO.1 of the original CALB; on the basis of a wild type lipase gene sequence, through rational design and site-specific mutagenesis, mutants S195F, S195Y, A283Y, S195F-A283Y and S195Y-A283Y, of which the Tm value and the optimal reaction temperature are obviously improved and the catalytic activity is improved by 1-2 times, are finally obtained; when the mutant is used for catalyzing synthesis of docosahexaenoic acid (DHA) glyceride, higher catalytic efficiency is shown, and the yield of the DHA glyceride is effectively improved.

The invention discloses a uricase mutant. The uricase sequence from aspergillus flavus and the uricase sequence from candida are taken as templates, multiple rounds of random mutation are carried out, sequencing verifies that the key amino acid site of the catalytic center is mutated, and the catalytic activity is enhanced. In-vivo and in-vitro uric acid reducing activities of the two uricase mutants are further verified, and the two uricase mutants can significantly reduce the content of uric acid and are expected to become new uricase drugs for treating hyperuricemia.

The invention provides a Candida viscera CATH2303 (Candida viscera CATH2303) and an application of the Candida viscera CATH2303. The Candida viscera CATH2303 The candida vesii provided by the invention has reduced fatty alcohol dehydrogenase activity or does not have fatty alcohol dehydrogenase activity, and can efficiently utilize fatty acid or derivatives thereof as a substrate to produce omega-hydroxy fatty acid, and the yield is obviously improved. The candida vesii and the method for producing the omega-hydroxy fatty acid by using the candida vesii, provided by the invention, have the potential of industrial large-scale production.

The invention relates to the technical field of cosmetics, and particularly discloses an anti-hair-loss composition as well as a preparation method and application thereof. The invention relates to a preparation method of an anti-hair loss composition, which comprises the following steps: cleaning and drying a fermentation substrate, crushing into slurry, sterilizing, inoculating lactobacillus plantarum, candida tropicalis, schizosaccharomyces cerevisiae and monascus purpureus strains at a proper temperature, carrying out sealed fermentation, and after fermentation is completed, carrying out centrifugal separation on the fermentation liquor to obtain the anti-hair loss composition. Taking supernate, sterilizing, and performing vacuum freeze drying to obtain the anti-alopecia composition. According to the application of the anti-hair-loss composition, the anti-hair-loss composition is added into a hair product. The anti-hair loss composition disclosed by the invention is natural in source, and can promote absorption of skin to active substances, so that the anti-hair loss composition has the effects of rapidly inhibiting scalp oil and reducing hair loss.

This invention discloses an overexpression Met15 This invention relates to a genetically engineered strain of *Candida utilis*, its construction method, and its applications, belonging to the field of bioengineering technology. The starting strain of the genetically engineered strain described in this invention is *Candida utilis*. C. utilis CCTCC NO: M 209298, strain overexpressing O-acetylhomoserine thioylase. Met15 Gene. This invention utilizes gene recombination technology to overexpress the O-acetylhomoserine sulfhydrylase encoding gene in *Candida utilis* (…). Met15 This can increase the content of L-methionine and L-cysteine ​​in yeast cells, increase the supply of sulfur-containing amino acid precursors, and ultimately increase the yield of SAM and GSH co-production in yeast cells.

The invention discloses a compound microorganism feed additive for promoting the growth of livestock and poultry. The compound microorganism feed additive comprises microorganism strains, natural plant components and auxiliary materials, the microbial strains comprise 8 to 12 parts of candida utilis, 5 to 9 parts of bacillus licheniformis, 2 to 4 parts of bacillus subtilis, 8 to 12 parts of aspergillus niger, 8 to 12 parts of lactobacillus delbrueckii, 8 to 12 parts of lactobacillus acidophilus, 8 to 12 parts of bifidobacterium and 3 to 7 parts of lactobacillus salivarius; the natural plant components comprise 0.5 to 2 parts of moringa seed powder; the auxiliary materials comprise 20 to 30 parts of cane sugar, 10 to 15 parts of fish meal, 2 to 3 parts of grease, 10 to 20 parts of soybean meal, 5 to 10 parts of pupa powder, 10 to 15 parts of zinc lactate, 1 to 2 parts of calcium gluconate, 3 to 5 parts of calcium carbonate and 2 to 4 parts of paeonol. Microbial strains in the compound microbial feed additive can generate various growth regulatory factors such as vitamins, amino acids and organic acids, so that the growth and development of livestock and poultry are stimulated, and the growth speed is increased.

The application belongs to the technical field of protein expression, and relates to a gene expression cassette for improving the yield of methionine of Candida utilis, an expression vector, genetically engineered bacteria, a method and application. The application provides application of a GAP-P mutant promoter GP6 and a homologous signal peptide SP8 in improving the yield of methionine of Candida utilis; the nucleotide sequence of the GP6 is shown in SEQ ID NO. 1, and the nucleotide sequence of the SP8 is shown in SEQ ID NO. 2. The application realizes the improvement of the yield of methionine through the improvement of the strength of the promoter and the screening of the homologous signal peptide.

The present application relates to the field of genetic engineering and bioengineering technology, in particular to a Candida antarctica lipase B mutant and application thereof. The Candida antarctica lipase B mutant provided by the present application is obtained by joint mutation of the 143th and 284th positions in the amino acid sequence shown in the wild type lipase B. The Candida antarctica lipase B mutant constructed by the present application has 12.5 times higher enzyme activity than the wild type enzyme, and the protein expression amount is as high as 1.8-1.9 g / L under the scale of 5 L fermenter, which can significantly improve the yield and reduce the production cost. In addition, the Candida antarctica lipase B mutant can be used for synthesis of high value-added, non-natural substrates such as vitamin C fatty acid ester and vitamin A oleate, which lays a foundation for the application of lipase in the field of fine chemical industry.

The invention discloses a preparation process of a multi-stage fermentation biological feed, and belongs to the technical field of feed biology. In order to solve the problems that the existing fermented feed is poor in strain synergism, insufficient in enzymolysis, low in probiotic colonization amount and the like, the method adopts a staged and time sequence inoculation strategy, and four stages of aerobic enzyme production degradation, aerobic fermentation and enzymolysis, facultative anaerobic fermentation and anaerobic probiotic colonization are sequentially carried out; and the inoculation sequence and proportion of trichoderma reesei, aspergillus niger, aspergillus oryzae, bacillus subtilis, candida utilis and lactobacillus plantarum are accurately controlled. By constructing an efficient flora metabolic chain, the quality of the feed is remarkably improved. The crude protein content of the prepared feed can reach 37.8% or above, and the viable count of lactobacillus plantarum reaches 3.1 * 10 < 10 > CFU / g. The prepared feed product has the advantages of high nutritional value, strong probiotic function and long shelf life, and the process is controllable and suitable for industrial production.

The invention discloses a method for rapidly detecting dichlorvos on site based on scattering turbidity, and belongs to the technical field of food and agricultural product safety detection. The method comprises the following steps: incubating dichlorvos with different concentrations with candida antarctica lipase, a phosphate buffer solution and L-ascorbyl palmitate, centrifugally incubating to obtain a reaction solution, taking a supernatant to react with a manganese dioxide nanosheet, measuring the turbidity of the reaction solution through a portable turbidimeter, and establishing a linear equation of the concentration and the turbidity of the dichlorvos; by measuring the turbidity of an unknown sample, the concentration of the dichlorvos can be calculated according to a linear equation. According to the method, invisible dichlorvos concentration signals are converted into measurable turbidity signals by utilizing the synergistic effect of enzyme inhibition reaction and turbidity change induction of the nano material, so that rapid and sensitive detection of dichlorvos is realized. The method is easy and convenient to operate, low in cost, high in detection speed, free of large instruments and especially suitable for on-site rapid screening of agricultural products.

The present application relates to a kind of containing fatty acyl-coa dehydrogenase engineering bacteria and its application method, belong to microbial fermentation technical field.The engineering bacteria containing fatty acyl-coa dehydrogenase gene in the present application include fatty acyl-coa dehydrogenase gene and fatty acyl-coa thioesterase gene, the fatty acyl-coa dehydrogenase gene is derived from Candida tropicalis or Yarrowia lipolytica, its nucleotide sequence is SEQ ID NO.1 or SEQ ID NO.2;The nucleotide sequence of the fatty acyl-coa thioesterase gene is SEQ ID NO.3.Fatty acyl-coa dehydrogenase from Candida tropicalis and Yarrowia lipolytica, combined with existing fatty acyl-coa thioesterase, jointly convert 10-hydroxy decanoic acid, significantly improve the conversion rate of 10-hydroxy-2-decenoic acid, so that the industrialization production of 10-hydroxy-2-decenoic acid becomes possible.

The present invention belongs to the field of microbial technology and discloses a strain of Candida and its application in the fermentation of Yunnan Camellia. Candida was isolated from Yunnan wild edible fungi (white boletus) Candida railenensis )BTN‑HB‑M5, which was deposited in the Guangdong Microbial Culture Collection Center on September 20, 2024, with the deposit number GDMCC No: 65164. The Yunnan Camellia flower fermentation extract can be obtained by using Candida BTN‑HB‑M5 through appropriate culture and collection processes. The overall fermentation process is green and efficient. The Yunnan Camellia flower fermentation extract prepared is obtained by using only Candida ( Candida railenensis The fermented product of Camellia sinensis (Yunnan Camellia) can be used in food and daily chemical products. It maximizes the preservation of the natural active ingredients of Camellia sinensis, without any added ingredients or irritating byproducts, making it suitable for use in daily chemical products. This Camellia sinensis fermented extract has excellent antioxidant, anti-glycation, and anti-aging effects, significantly alleviating skin aging indicators through antioxidant activity, and has great application prospects in the field of daily chemical products.

The present invention provides a diglyceride oil with hypoglycemic effect and a preparation method thereof, which relates to the technical field of functional oil processing. The preparation method comprises the following steps: mixing seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil, adding glycerol and water to obtain material A; adding Pseudomonas cepacia lipase and Candida antarctica lipase B to material A for a primary reaction to obtain material B; adding Candida lipolytica lipase and Candida antarctica lipase B to material B for a second reaction to obtain material C; adding Thermomyces lanuginosus lipase to material C for a third reaction to obtain material D; and treating the reacted material D to obtain the diglyceride oil. The diglyceride oil prepared by the present invention has a good effect in reducing blood sugar.

The invention discloses a targeted compound probiotic preparation for producing SOD (superoxide dismutase) eggs, which is prepared from the following active ingredients in percentage by mass: 5 to 15 percent of bacillus licheniformis, 3 to 10 percent of pseudomonas strain NJ-62, 15 to 30 percent of saccharomyces boulardii, 10 to 20 percent of lactobacillus plantarum, 8 to 15 percent of bacillus subtilis, 10 to 20 percent of candida utilis and 5 to 15 percent of lactobacillus acidophilus. The invention relates to the technical field of healthy breeding of livestock and poultry and production of functional food, in particular to integrated production of'safety 'and'function', specifically, targeted biological detoxification and nutrition metabolism induction technologies are deeply integrated for the first time, and complex probiotic groups remove antibiotics, heavy metals and other endogenous and exogenous pollutants in advance, so that a'clean 'physiological environment is created for laying hens; on the basis, efficient synthesis of SOD is specifically induced, and the problem of potential safety hazards of the functional eggs is fundamentally solved.

The application discloses a Candida antarctica lipase B mutant and a preparation method thereof, and belongs to the technical field of biology. Eleven mutants with improved enzyme activity are obtained, including six unit point mutants and five combined mutants; compared with a wild type, the enzyme activity of the mutants Q11L, F71N, F118Y, V149T, L219N, T244D, S31T / F71N, F71N / F118Y, F71N / L219N, S31T / F71N / F118Y and F71N / F118Y / L219N is improved, and is respectively increased by 18.45% to 240.88%; the CALB mutant with high enzyme activity is obtained, and has great industrial application potential and economic value.

The invention relates to ceramide liposome as well as a preparation method and application thereof. The ceramide liposome is prepared from the following raw materials: water, an alcohol solvent, phospholipid, cholesterol, a co-emulsifier and an active component, the active components comprise ceramide and a candida albicans / glucose / flat nuclear wood oil fermentation product. The invention develops a liposome product simultaneously loading ceramide and candida albicans / glucose / flat nuclear wood oil fermentation products, the two active components are mutually matched and have synergistic interaction in the aspect of skin barrier repair, the transdermal permeation effect of the active components can be improved by virtue of a liposome form, and the bioavailability of the liposome product is improved. Compared with unfermented flat nuclear wood oil, the candida albicans / glucose / flat nuclear wood oil fermentation product is selected, so that the hydrophilicity and stability of the liposome can be improved, the encapsulation performance of the liposome on ceramide and the transdermal permeation effect of the ceramide can be improved, and finally the skin barrier repairing effect of the liposome product is improved.