The invention discloses a polyethyleneimine based chloramines type antibacterial cellulose membrane, a preparation method and application. The preparation method comprises the following steps: step 1,preparing a cellulose solution with the mass concentration being 5wt percent; step 2, performing casting on a base plate to form a regenerated cellulose membrane after the cellulose solution is defoamed, and washing after solidification, so as to obtain a regenerated cellulose gel membrane; step 3, oxidizing the regenerated cellulose gel membrane through a sodium periodate aqueous solution, so asto obtain dialdehyde cellulose gel membrane with the aldehyde group amount being 1 to 3mmol/g; step 4, dipping the dialdehyde cellulose gel membrane into a polyethyleneimine aqueous solution to perform grafting reaction, so as to obtain a polyethyleneimine grafted cellulose membrane; step 5, chloridizing the polyethyleneimine grafted cellulose membrane, so as to obtain the polyethyleneimine basedchloramines type antibacterial cellulose membrane. The preparation method is performed in an aqueous solution, reaction conditions are mild, the technology is simple to operate, and the preparation processes are environmentally friendly; the formed antibacterial cellulose membrane has good biocompatibility and breathability, and the antibacterial and bactericidal properties are excellent.