Method for removing protein in polysaccharide extraction liquid by dialdehyde cellulose

Abstract

The invention provides a novel method for removing protein in the polysaccharide separation and purification process. The method comprises the following steps of firstly preparing a polysaccharide solution at a certain weight percentage; then, adding dialdehyde cellulose at different oxidization degrees in the polysaccharide solution; controlling the material-liquid ratio (g / mL) and the reaction temperature; regulating the pH value; performing reaction for a certain time at the specified temperature; after the reaction is completed, performing cooling and centrifugal dispersion; taking supernate; repeating the operation for many times to obtain the protein removal polysaccharide solution. The method provides a new idea for the protein removal method; in addition, the process is simple; the operation is easy; meanwhile, the raw material cellulose has the advantages of rich resources, environment-friendly performance and the like; the method belongs to a green and environment-friendly protein removal method and is favorable for the scaled separation and purification of crude polysaccharide.

Description

technical field [0001] The invention relates to a new method for removing protein in the process of separating and purifying polysaccharides. It particularly relates to a deproteinization method that does not use traditional chemical or enzymatic methods, but uses dialdehyde cellulose to react with protein to generate Schiff base for deproteinization, which belongs to the innovative field of deproteinization methods. Background technique [0002] Polysaccharides widely exist in various animal, plant and microbial tissues, and have become a research hotspot in natural medicine due to their diverse biological activities. However, impurity proteins are often contained in polysaccharide solutions, so how to effectively remove proteins has become a very meaningful research content. Wang Shan and Huang Shengyang reviewed the methods of deproteinization in plant polysaccharide extracts in "Research Progress on Deproteinization Methods of Plant Polysaccharide Extracts", and compreh...

AI Technical Summary

Problems solved by technology

The reagents used for Sevage deproteinization are chloroform and n-butanol, and chloroform is a toxic substance, which is likely to cause a decrease in the activity of polysaccharides and solvent residues. This method is time-consuming to operate, consumes a lot of organic solvents, has high costs, and has great environmental pollution and harm to operators. more damage

The precipitation phenomenon in the deproteinization operation of the trichloroacetic acid method is more obvious than that of the Sevage method, but more polysaccharides are easily lost during deproteinization, and the resolubility of polysaccharides after deproteinization is significantly weakened, which has a greater impact on the structure of polysaccharides. Therefore, the deproteinization effect of this method poor

Although the effect of hydrochloric acid deproteinization is better, if the experimental conditions are not strictly controlled, the loss rate of polysaccharides is high, and there are disadvantages of polysaccharide hydrolysis

The enzymatic method is to use protease to hydrolyze the protein in the polysaccharide extract under the catalysis of protease, thereby removing the protein. The specificity of the protease method makes the effect unstable, and it may also destroy the structure of the proteoglycan complex and affect its efficacy. , and proteases are expensive, resulting in a higher cost for this method

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