Preparation method of oxidized dialdehyde cellulose immobilized urease

Abstract

The invention provides a preparation method of oxidized dialdehyde cellulose immobilized urease. The preparation method comprises the steps of preparation of oxidized dialdehyde cellulose and preparation of immobilized urease, wherein the step of preparation of oxidized dialdehyde cellulose comprises the substeps of activation of cellulose and preparation of the oxidized dialdehyde cellulose through hydrothermal synthesis; and the step of preparation of immobilized urease comprises the substeps of preparation of a dispersed phase, immobilization of urease and washing and drying. The preparation method is characterized by utilizing the cellulose widely existing in the nature as a matrix material to prepare an oxidized dialdehyde cellulose carrier through hydrothermal synthesis and utilizing Schiff base reaction to immobilize urease on the carrier, thus obtaining the oxidized cellulose immobilized urease. The preparation method has the following beneficial effects: the prepared oxidizeddialdehyde cellulose has higher content of aldehyde groups, thus providing more active sites for urease bonding; the method of immobilizing urease is simple and is easy to operate; and the carrier has wide material sources. Thus, the preparation method has wide application prospects and practical values.

Description

Technical field [0001] The invention relates to an enzymatic technology, in particular to a preparation method of immobilized urease using oxidized dialdehyde cellulose as a carrier. Background technique [0002] Urease has high potential value in sewage treatment, wine making and medical fields. However, due to the high price of urease, the urease in the solution is easily denatured and inactivated, and the urease after the reaction is difficult to recover and cannot be reused, which greatly limits the application of urease. In order to improve the stability, reusability and storage stability of urease in solution, it is a feasible technical approach to connect and fix urease to the carrier by a proper method to overcome the shortcomings of free urease. [0003] At present, the choice of immobilized carrier materials for urease is relatively wide, mainly divided into inorganic materials and organic materials. Among them, organic materials include polymers (such as modified polyac...

AI Technical Summary

Problems solved by technology

However, due to the lack of functional groups that can bind to enzymes in this type of carrier, the enzyme can only be immobilized on the carrier by adsorption, and the resulting immobilized urease has weak reusability.

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