Preparation method of oxidized dialdehyde cellulose immobilized urease
A technology of dialdehyde cellulose and cellulose, applied in the field of enzymology, can solve the problems of lack of functional groups, weak reusability of immobilized urease, etc., and achieve the effects of sufficient oxidation reaction, simple method and mild conditions
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[0016] The present invention is described in further detail in conjunction with the embodiment:
[0017] Urease is an enzyme that specifically decomposes urea, also known as amidohydrolase. In the presence of water, urea is decomposed into ammonia and carbon dioxide, which is 10 times faster than the general urea decomposition reaction (without urease catalysis). 14 times. Therefore, urease has high potential value in sewage treatment, brewing and medical fields. However, due to the high price of urease, the urease in the solution is easily denatured and inactivated, and the urease after the reaction is difficult to recover and cannot be reused, which greatly limits the application of urease. In order to improve the stability, reusability and storage stability of urease in solution, an appropriate method can be used to bond and immobilize urease with a suitable carrier, so as to well overcome the shortcomings of free urease. There are a wide range of immobilized carrier mat...
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