Preparation method of dialdehyde cellulose grafted epsilon-polylysine antibacterial material

A technology of dialdehyde cellulose and polylysine, applied in botany equipment and methods, chemicals for biological control, animal repellants, etc., can solve problems such as limiting the scope of application, and achieve wide application and distribution Wide range of antibacterial effect

Inactive Publication Date: 2019-03-19
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the water-soluble characteristics of ε-polylysine, the scope of application is greatly limited.

Method used

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  • Preparation method of dialdehyde cellulose grafted epsilon-polylysine antibacterial material
  • Preparation method of dialdehyde cellulose grafted epsilon-polylysine antibacterial material
  • Preparation method of dialdehyde cellulose grafted epsilon-polylysine antibacterial material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Dissolve ε-polylysine in 100mL deionized water, adjust the medium to pH 8.0 with NaOH solution, and add bisaldehyde according to the molar ratio of aldehyde group of dialdehyde cellulose to ε-polylysine 1:0.1. Aldehyde cellulose, and then carry out grafting reaction at 30 ℃ for 8h;

[0032] (2) After the reactor is cooled to room temperature, the filtrate is removed by suction filtration, the filter cake is washed with deionized water and ethanol successively, the obtained graft is naturally air-dried, ground evenly, and then vacuum-dried at 37°C to obtain the graft ε-polylysine antibacterial material.

[0033] (3) antibacterial material is carried out antibacterial experiment to sarcinina;

[0034] The minimum inhibitory concentration (MIC) of the dialdehyde cellulose / ε-PL antibacterial material prepared from dialdehyde cellulose with an aldehyde content of 5.514 mmol / g against Sarcina was 30 mg / mL.

Embodiment 2

[0036] (1) Dissolve ε-polylysine in 100mL deionized water, adjust the medium to pH 8.5 with NaOH solution, and add dialdehyde cellulose according to the molar ratio of aldehyde group of dialdehyde cellulose to ε-polylysine 1:0.1. Aldehyde cellulose, and then carry out grafting reaction at 30 ℃ for 8h;

[0037] (2) After the reactor is cooled to room temperature, the filtrate is removed by suction filtration, the filter cake is washed with deionized water and ethanol successively, the obtained graft is naturally air-dried, ground evenly, and then vacuum-dried at 37°C to obtain the graft ε-polylysine antibacterial material.

[0038] (3) Antibacterial material is carried out antibacterial experiment to Escherichia coli;

[0039] The minimum inhibitory concentration (MIC) of the dialdehyde cellulose / ε-PL antibacterial material prepared from dialdehyde cellulose with an aldehyde content of 5.514 mmol / g against E. coli was 7.5 mg / mL.

Embodiment 3

[0041] (1) Dissolve ε-polylysine in 100mL deionized water, adjust the medium to pH 8.5 with NaOH solution, and add dialdehyde cellulose according to the molar ratio of aldehyde group of dialdehyde cellulose to ε-polylysine 1:0.2 Aldehyde cellulose, and then carry out grafting reaction at 30 ℃ for 8h;

[0042] (2) After the reactor is cooled to room temperature, the filtrate is removed by suction filtration, the filter cake is washed with deionized water and ethanol in turn, the obtained graft is naturally air-dried, ground evenly, and then vacuum-dried at 47°C to obtain the graft ε-polylysine antibacterial material.

[0043] (3) Antibacterial material is carried out antibacterial test to Staphylococcus aureus;

[0044] The minimum inhibitory concentration (MIC) of dialdehyde cellulose / ε-PL antibacterial material against Staphylococcus aureus was 7.5 mg / mL prepared from dialdehyde cellulose with aldehyde group content of 5.514 mmol / g.

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Abstract

The invention relates to dialdehyde cellulose grafted epsilon-polylysine antibacterial material, prepared by oxidizing cellulose into dialdehyde cellulose, and using the dialdehyde cellulose as a supporter to modify with epsilon-polylysine under a certain aldehyde group molar ratio through grafting reaction so that epsilon-polylysine is grafted to dialdehyde microcrystalline cellulose. The processto prepare the dialdehyde cellulose grafted epsilon-polylysine antibacterial material is simple; the dialdehyde cellulose grafted epsilon-polylysine antibacterial material has no toxic and side effects and has good antibacterial activity; with penicillin acting as a positive control and LB (lysogeny broth) liquid medium as a negative control, the material herein has minimum inhibitory concentrations of 7.5 mg/mL, 15 mg/mL and 30 mg/mL respectively for E. coli and S. aureus, S. typhimurium, and S. lutea and B. subtilis. A new idea is provided for the research and development of novel antibacterial materials.

Description

[0001] The invention relates to a preparation method of a bacteriostatic material grafted with dialdehyde cellulose and ε-polylysine to inhibit bacterial growth. Using dialdehyde cellulose with a certain degree of oxidation, according to a certain molar ratio of aldehyde groups, grafting reaction with ε-polylysine (ε-PL), by increasing the positive charge on the graft, it is negatively charged with the bacterial cell wall Combination of substances, thereby inhibiting bacterial growth, belongs to the innovative field of antibacterial material synthesis. Background technique [0002] Cellulose is the most abundant natural renewable polysaccharide in nature. It is one of the important biomass materials. It has the characteristics of biodegradability and biocompatibility; it has the advantages of low cost, wide application and sustainable development. However, the scope of application of cellulose is limited due to its own structure and the interaction between molecular chains, su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00A01N43/16A01P1/00
CPCA01N43/16C08G81/00
Inventor 张黎明李妍时文佳何希宏何志娜戴玉杰彭巧玲
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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