A kind of lipase mutant and its use

A mutant and lipase technology, applied in the field of lipase mutants and their applications, can solve the problems of low enantioselectivity and achieve the effect of improving enantioselectivity
CN103881992BActive Publication Date: 2017-04-26SHENYANG PHARMA UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHENYANG PHARMA UNIVERSITY
Publication Date
2017-04-26

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Abstract

The invention relates to the technical field of biology, and relates to a lipase mutant and uses thereof. The mutant is obtained from wild type candida antarctica lipase B (CALB) through mutation. The invention particularly relates to the lipase mutant, a preparing method thereof, and a method of preparing S-configuration ibuprofen type compounds with optical activity through catalysis of hydrolysis of ester derivatives of the ibuprofen type compounds by utilization of the lipase mutant and through kinetic resolution. The wild type candida antarctica lipase B is subjected to mutation to obtain the modified lipase. The lipase is expressed in pichia pastoris engineering bacteria. The lipase comprises an amino acid sequence having at least 85% of identity with the SEQ ID NO.02. The 189 site residue corresponding to the SEQ ID NO.02 of the lipase is an aromatic amino acid residue and the 190 site residue corresponding to the SEQ ID NO.02 of the lipase is a nonpolar amino acid residue. By subjecting the wild type candida antarctica lipase B to mutation, the enantioselectivity of the lipase to the ibuprofen type compounds is improved.
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Description

technical field

[0001] The present invention relates to the field of biotechnology, and relates to a lipase mutant and its application. The mutant is obtained through mutation from wild-type Candida antarctica lipase B (CALB), and specifically relates to a lipase mutant And its preparation method and using the lipase mutant to catalyze the hydrolysis of the ester derivatives of ibuprofen compounds, kinetic resolution to obtain the optically active S Method of configuring ibuprofen-like compounds. Background technique

[0002] Lipase, namely triacylglycerol acyl hydrolase, is a special ester bond hydrolase, which can catalyze the hydrolysis reaction of oil at the oil-water interface to generate fatty acid and glycerol, monoglyceride or diester. Lipase is usually used to catalyze the hydrolysis reaction of ester compounds in the aqueous phase system, catalyze the esterification and transesterification of alcohol compounds in the non-aqueous phase system, and catalyze the amin...

Claims

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